A5030979504- RNA Interference and Gene Delivery
- Reinforcement Learning in Robotics
- Distributed Control Multi-Agent Systems
- Environmental Sustainability in Business
- Energy, Environment, Economic Growth
- Robotic Path Planning Algorithms
- Nanowire Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Sustainable Supply Chain Management
- Microfluidic and Bio-sensing Technologies
- Graphene and Nanomaterials Applications
- Quantum Dots Synthesis And Properties
- Neuroscience and Neural Engineering
- CRISPR and Genetic Engineering
- Microfluidic and Capillary Electrophoresis Applications
- Corporate Social Responsibility Reporting
- Monoclonal and Polyclonal Antibodies Research
- Optimization and Search Problems
- Modular Robots and Swarm Intelligence
- Distributed systems and fault tolerance
- Transportation and Mobility Innovations
- Economic and Technological Innovation
- Advanced Memory and Neural Computing
- Drug Transport and Resistance Mechanisms
- Semiconductor materials and interfaces
Gaozhou People's Hospital
2025
National Center for Nanoscience and Technology
2021-2025
Chinese Academy of Sciences
2010-2025
University of Chinese Academy of Sciences
2023-2025
National University of Singapore
2021-2024
Guangdong Pharmaceutical University
2023-2024
Shanghai Jiao Tong University
2021-2024
Shanghai University
2016-2024
First Affiliated Hospital of Guangdong Pharmaceutical University
2023-2024
Renji Hospital
2024
Nondestructive introduction of genes, proteins, and small molecules into mammalian cells with high efficiency is a challenging, yet critical, process. Here we demonstrate simple nanoelectroporation platform to achieve highly efficient molecular delivery transfection yields excellent uniformity cell viability. The system built on alumina nanostraws extending from track-etched membrane, forming an array hollow nanowires connected underlying microfluidic channel. Cellular engulfment the...
CD4(+)CD25(+) regulatory T cells (Treg cells) are important in maintenance of peripheral tolerance. The direct effect Treg on macrophages was studied using a mouse model which syngeneic were adoptively transferred into the peritoneal cavity SCID mice. Peritoneal mice with expressed significantly higher levels CD23, CD47 and CD206 less CD80 major histocompatibility complex class II molecules as compared those that received either CD4(+)CD25(-) or no cells. Macrophages injected displayed...
Significance Efficient nonviral delivery of macromolecules including mRNA, DNA plasmids, Cas9 ribonucleoproteins, and functional protein into both adherent cells suspension with high cell viability is crucial for cellular manipulation, imaging, medical applications. However, the conventional methods are limited to a certain range molecules types often reduce viability. Here, we demonstrate effective by nanopore electroporation (NanoEP) using water-filter nanoporous membrane. As compared that...
Significance Cell content analysis has rapidly become one of the most important new tools for measuring cell phenotype and behavior. However, central limitation current sampling technologies is they are destructive must lyse cells to measure contents. This destruction prevents knowledge prior or future states cell, which particularly dynamic processes, such as development differentiation. Here, we show a nondestructive longitudinal platform that can sample repeatedly accurately from same...
Intracellular delivery of mRNA, DNA, and other large macromolecules into cells plays an essential role in array biological research clinical therapies. However, current methods yield a wide variation the amount material delivered, as well limitations on cell types cargoes possible. Here, we demonstrate quantitatively controlled range primary lines with tight dosage distribution using nanostraw-electroporation system (NES). In NES, are cultured onto track-etched membranes protruding...
RNA interference, which involves the delivery of small interfering (siRNA), has been used to validate target genes, understand and control cellular metabolic pathways, use as a "green" alternative confer pest tolerance in crops. Conventional siRNA methods such viruses Agrobacterium-mediated exhibit plant species range limitations uncontrolled DNA integration into genome. Here, we synthesize polyethylenimine-functionalized gold nanoclusters (PEI-AuNCs) mediate intact plants show that these...
Hyperuricemia is associated with an increased risk of gout, hypertension, diabetes, and cardiovascular diseases. Most mammals maintain normal serum uric acid (SUA) via urate oxidase (Uox), enzyme that metabolizes poorly-soluble UA to highly-soluble allantoin. In contrast, Uox became a pseudogene in humans apes over the long course evolution. Here we demonstrate atavistic strategy for treating hyperuricemia based on endogenous expression hepatocytes mediated by mRNA (mUox) loaded ionizable...
Pluronic F127 (F127), an amphiphilic triblock copolymer, has been shown to have significant potential for drug delivery, as it is able incorporate hydrophobic drugs and self-assemble into nanosize micelles. However, suffers from dissociation upon dilution owing the relatively high critical micelle concentration lack of stimuli-responsive behavior. Here, we synthesized α-tocopherol (TOC) modified polymer (F127-SS-TOC) via a redox-sensitive disulfide bond between TOC, which formed stable...
The oral route is preferred for systemic drug administration and provides direct access to diseased tissue of the gastrointestinal (GI) tract. However, many drugs have poor absorption upon due damaging enzymatic pH conditions, mucus cellular permeation barriers, limited time dissolution. To overcome these limitations enhance absorption, micron-scale devices with planar, asymmetric geometries, termed microdevices, been designed adhere lining GI tract release at high concentrations directly...
Vaccines based on messenger RNA (mRNA) are attracting worldwide attention as the Pfizer and Moderna vaccines have been authorized for emergency use by U.S. Food Drug Administration similar authorities around world. This is first time that mRNA-based ever approved healthy population [[1]Polack F.P. et al.Safety efficacy of BNT162b2 mRNA Covid-19 vaccine.N Engl J Med. 2020; https://doi.org/10.1056/NEJMoa2034577Crossref Scopus (4574) Google Scholar], marks a critical milestone achievement in...
Abstract RNA interference (RNAi) is a powerful tool for understanding and manipulating signaling pathways in plant science, potentially facilitating the accelerated development of novel traits crop yield improvement. The common strategy delivering siRNA into intact plants using agrobacterium or viruses complicated time‐consuming, limiting application RNAi research. Here, delivery method based on mesoporous silica nanoparticles (MSNs) reported, which allows efficient mature leaves via topical...
Objective: To evaluate the clinical outcomes of combined complete preservation chordal structure mitral valve replacement (C-MVR) with total anatomical arterial myocardial revascularization (TACR) in coronary patients moderate-to-severe or severe ischemic regurgitation (IMR). Methods: This is a retrospective multi-center case series study. Data were retrospectively collected from 127 artery disease moderate to IMR who received TACR C-MVR July 2015 April 2024 13 hospitals China. There 90...
The Multi-Agent Path Finding (MAPF) problem aims to determine the shortest and collision-free paths for multiple agents in a known, potentially obstacle-ridden environment. It is core challenge robotic deployments large-scale logistics transportation. Decentralized learning-based approaches have shown great potential addressing MAPF problems, offering more reactive scalable solutions. However, existing methods usually rely on making decisions based limited field of view (FOV), resulting...
Using the Chinese automotive industry as research subject, this study constructs a supply chain network based on real transaction data of business interactions between upstream and downstream enterprises in sector from 2011 to 2020. It analyzes topological properties structure network, simulates risk-bearing capacity under risk shocks, focuses exploring impact firm heterogeneity within credit contagion. The findings reveal that there are differences complexity networks procurement sales...
Synthetic circular RNA (circRNA) holds great promise for biomedical research and therapeutic applications, but impurities introduced during synthesis trigger innate immune responses significantly compromise its efficacy. In this study, key immunogenic byproducts, including double-stranded RNA, 5' triphosphates from uncircularized hydrolyzed fragments, are identified as impairing circRNA functionality via RNA-sensing pathways. To address this, a multi-step purification process is developed...
With the advent of big-data era, conventional memory technologies and devices are facing enormous challenges. Resistive random access (RRAM) is an emerging technology that has aroused widespread interest for its immense potential. However, there remain some problems in resistive switching devices, such as high voltages, voltages distribution, wide variation resistance states, poor endurance. In this work, molybdenum disulfide quantum dots applied to devices. The resulting exhibit improved...
High purity is essential in mRNA-based therapeutic applications. A major contaminant of vitro-transcribed (IVT) mRNA manufacturing double-stranded RNA (dsRNA), which can induce severe anti-viral immune responses. Detection methods, such as agarose gel electrophoresis, ELISA, and dot-blot assay, are used to detect the existence dsRNA IVT products. However, these methods either not sensitive enough or time-consuming. To overcome challenges, we develop a rapid, sensitive, easy-to-implement...
In autonomous robot exploration tasks, a mobile needs to actively explore and map an unknown environment as fast possible. Since the is being revealed during exploration, frequently re-plan its path online, new information acquired by onboard sensors used update partial map. While state-of-the-art planners are frontier- sampling-based, encouraged recent development in deep reinforcement learning (DRL), we propose ARiADNE, attention-based neural approach obtain real-time, non-myopic planning...
Abstract Nanowires have a wide variety of biomedical applications, including drug delivery, cellular manipulation, interfacing, and biosensing. Specifically, hollow nanowire arrays, or “nanostraws,” are recently developed to penetrate cell membranes provide temporal dose controlled delivery biomolecules into the cells. The fabrication nanostraw arrays uses nanoporous as starting templates. However, with custom‐designed diameters densities often expensive commercially unavailable. As result,...