A5002837202- Hormonal Regulation and Hypertension
- Adrenal Hormones and Disorders
- Topology Optimization in Engineering
- Coronary Interventions and Diagnostics
- Neuropeptides and Animal Physiology
- Atherosclerosis and Cardiovascular Diseases
- Metabolism and Genetic Disorders
- Cardiac Structural Anomalies and Repair
- Total Knee Arthroplasty Outcomes
- Metallurgy and Material Forming
- Prosthetics and Rehabilitation Robotics
- Knee injuries and reconstruction techniques
- Adipose Tissue and Metabolism
- Cancer, Hypoxia, and Metabolism
- Mechanical Engineering and Vibrations Research
- SARS-CoV-2 and COVID-19 Research
- Blood Pressure and Hypertension Studies
- Manufacturing Process and Optimization
- Cardiac Imaging and Diagnostics
- Chemokine receptors and signaling
- Neonatal Health and Biochemistry
- Antiplatelet Therapy and Cardiovascular Diseases
- Renin-Angiotensin System Studies
- Signaling Pathways in Disease
- Scoliosis diagnosis and treatment
Hokkaido University
2009-2023
Noto General Hospital
2021
Imperial College London
2012-2014
National Institute of Advanced Industrial Science and Technology
2010
The University of Tokyo
2003-2008
Takeda (United States)
2005
Kanazawa University
1979-1985
Background— AMP-activated protein kinase (AMPK) is a stress-activated that works as metabolic sensor of cellular ATP levels. Here, we investigated whether AMPK signaling has role in the regulation angiotensin II (Ang II)-induced proliferation signal rat vascular smooth muscle cells (VSMCs). Methods and Results— Aminoimidazole-4-carboxamide-1-β-ribofuranoside (AICAR) activated VSMCs inhibited Ang II-induced extracellular signal-regulated 1/2 phosphorylation but not p38 MAPK or Akt/PKB....
Recent studies have demonstrated a relationship between oral bacteria and systemic inflammation. Endothelial cells (ECs), which line blood vessels, control the opening closing of vascular barrier contribute to hematogenous metastasis; however, role bacteria-induced inflammation in tumor metastasis remains unclear. In this study, we examined phenotypic changes ECs following Streptococcus mutans (S. mutans) stimulation vitro vivo. The expression molecules associated with barrier-associated...
Serum antioxidant activity (AOA) was examined in 35 healthy subjects and 111 patients with chronic renal failure (CRF), consisting of 13 the predialysis stage, 11 requiring start regular dialysis therapy (RDT) 87 undergoing RDT. AOA determined by assaying serum to inhibit malondialdehyde (MDA) generation. levels were significantly lower CRF patients, lowest noticed uremic symptoms These restored a subnormal level during Defective appears be an endogenous metabolic consequence uremia. Sera...
We examined whether ANG II and TNF-alpha cooperatively induce vascular inflammation using the expression of monocyte chemoattractant protein (MCP)-1 as a marker inflammation. stimulated MCP-1 in synergistic manner smooth muscle cells. II-induced was potently inhibited to nonstimulated basal level by blockade p38-dependent pathway but only partially NF-kappaB-dependent pathway. In contrast, TNF-alpha-induced suppressed NF-kappaB activation modestly p38 activation. II- NF-kappaB- pathways...
Cetylpyridinium chloride (CPC), a quaternary ammonium compound, which is present in mouthwash, effective against bacteria, fungi, and enveloped viruses. This study was conducted to explore the antiviral effect of CPC on SARS-CoV-2. There are few reports wild-type SARS-CoV-2 at low concentrations such as 0.001%-0.005% (10-50 µg/mL). Interestingly, we found that suppressed infectivity human isolated strains (Wuhan, Alpha, Beta, Gamma) even saliva. Furthermore, demonstrated shows...
Growth hormone and IGF-1 have been suggested to tissue-protective effects. Ghrelin is a stomach-derived growth secretagogue. The effects of ghrelin on ischemia/reperfusion-induced renal failure in mice were examined. Ischemic acute was induced by bilateral artery clamping for 45 min reperfusion 24 h. (100 microg/kg mouse) or vehicle injected subcutaneously six times before surgery three after every 8 Twenty-four hours reperfusion, the right kidney isolated perfused. Acetylcholine (ACh)-...
Abstract Tumor endothelial cells (TEC) lining tumor blood vessels actively contribute to progression and metastasis. In addition cells, TEC may develop drug resistance during cancer treatment, allowing the survive chemotherapy metastasize. We previously reported that TECs resist paclitaxel treatment via upregulation of ABCB1. However, whether phenotypes are altered by anticancer drugs remains be clarified. Here, we show ABCB1 expression increases after in urothelial carcinoma cases. The...
Background— It is well known that diabetes mellitus a major risk factor for vascular diseases such as atherosclerosis and restenosis after angioplasty. has become clear advanced glycation end products (AGE) their receptor (RAGE) are implicated in diseases, especially mellitus. Nevertheless, the mechanisms by which often associated with remain unclear. Methods Results— To study role of endogenous cytokines tumor necrosis factor-α (TNF-α) interleukin-6 development expression RAGE, we used...
To study the mechanisms of vascular dysfunction in diabetes mellitus, we examined responses aorta to adrenomedullin (AM) and ANG II obese Zucker (OZ), lean (LZ), OZ rats administered fluvastatin (OZ + Flu). AM-induced endothelium-dependent vasorelaxation was impaired compared with LZ rats, restored AM-induced, (%Deltatension at 10(-7) mol/l AM; LZ, -85.1 +/- 3.1%; OZ, -50.7 2.5%; Flu, -75.6 2.7%). Expression endothelial nitric oxide synthase (eNOS) Akt phosphorylation response AM (10(-7)...
Although the role of calcineurin-dependent pathway in development cardiac hypertrophy has been intensively studied, little is known its vascular inflammatory diseases such as atherosclerosis and restenosis after angioplasty. To help elucidate calcineurin inflammation, we infected cultured smooth muscle cells (VSMCs) with an adenovirus construct expressing a constitutively active mutant calcineurin, examined effect on expression monocyte chemoattractant protein-1 (MCP-1). We also vivo using...
Although it has been established that myocyte enhancer factor 2 (MEF2) plays pivotal roles in the development of cardiovascular system as well skeletal muscle cells, little is known its role vascular inflammatory diseases such atherosclerosis and restenosis after angioplasty. To investigate MEF2 inflammation p38 activation MEF2, we infected cultured rat smooth cells (VSMCs) with an adenovirus construct expressing a dominant-negative mutant MEF2A (MEF2ASA) or mitogen-activated protein kinase...
Abstract Thrombosis is the major cause of death in severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection, and pathology vascular endothelial cells (ECs) has received much attention. Although there evidence infection ECs human autopsy tissues, their detailed pathophysiology remains unclear due to lack animal model study it. We used a mouse‐adapted SARS‐CoV‐2 virus strain young mid‐aged mice. Only mice developed fatal pneumonia with thrombosis. Pulmonary were isolated from...
To investigate the clinical outcomes of paclitaxel-eluting stents (PES) and sirolimus-eluting (SES) in patients on dialysis.Between May 2004 December 2008, 95 dialysis with 124 lesions were treated PES alone, compared to 184 244 SES retrospectively. One-year major adverse cardiac event (MACE) including stent thrombosis, target lesion revascularisation (TLR), myocardial infarction (MI) death compared. Baseline characteristics similar except for previous CABG (p = 0.02) reference vessel...
Tumor blood vessels play a key role in tumor metastasis. We have previously reported that endothelial cells (TECs) exhibit abnormalities compared to normal cells. However, it is unclear how TECs acquire these abnormalities. secrete extracellular vesicles (EVs) create suitable environment for themselves. identified miR-1246 be more abundant high metastatic melanoma EVs than low EVs. In the current study, we focused on as primarily responsible acquiring and examined whether alteration of cell...
Adrenomedullin exerts not only vasodilatory effects, but also angiogenic effects. In the present study, we investigated effects of adrenomedullin on collateral formation and circulating bone-marrow-derived cells after acute tissue ischaemia. Bone marrow 8–10-week-old female C57BL/6J mice was replaced with that from GFP (green fluorescent protein) transgenic (GFP mice). At 8 weeks transplantation, hindlimb ischaemia induced by resecting right femoral artery a plasmid expressing human (50 μg)...
The objective of the present study was to compare effects imidapril hydrochloride, an angiotensin converting enzyme inhibitor, and dilazep antiplatelet agent, on urinary protein excretion renal function in patients with chronic glomerulonephritis. Imidapril (2.5 or 5 mg/day) (300 450 administered for 3 years. Blood pressure, proteinuria, were measured before during treatment. In group (n = 11), decreased by approximately 50% (2.16 +/- 1.57 versus 0.90 0.53 g/g Cr, P < 0.01) blood pressure...
It is well known that excessive intake of sodium chloride (sodium) a risk factor for cardiovascular disease because it raises blood pressure. However, loading reportedly promotes independently its effect on To examine the mechanisms by which vascular inflammation pressure, we examined role calcineurin in loading-induced using wire injury model rat femoral artery. Calcineurin mRNA expression wire-injured artery was significantly higher sodium-loaded normotensive rats, such as Wistar-Kyoto...