A5082712873- Mesenchymal stem cell research
- Atherosclerosis and Cardiovascular Diseases
- Neuropeptides and Animal Physiology
- MicroRNA in disease regulation
- Hormonal Regulation and Hypertension
- Blood Pressure and Hypertension Studies
- Epigenetics and DNA Methylation
- Adipose Tissue and Metabolism
- Chronic Kidney Disease and Diabetes
- RNA Interference and Gene Delivery
- Coronary Interventions and Diagnostics
- RNA Research and Splicing
- Organ Donation and Transplantation
- Renal and related cancers
- Signaling Pathways in Disease
- Chemokine receptors and signaling
- Birth, Development, and Health
- Extracellular vesicles in disease
- Nitric Oxide and Endothelin Effects
- Ion Transport and Channel Regulation
- Angiogenesis and VEGF in Cancer
- Renal Diseases and Glomerulopathies
- Electrospun Nanofibers in Biomedical Applications
- Renal and Vascular Pathologies
- Immune cells in cancer
The University of Tokyo
2013-2022
St. Marianna University School of Medicine
2015-2016
University of Tokyo Hospital
2016
Creative Research Enterprises (United States)
2015
Pediatric Nephrology of Alabama
2005
Vanderbilt University
1995
Members of the miR-200 family micro RNAs (miRNAs) have been shown to inhibit epithelial-mesenchymal transition (EMT). EMT tubular epithelial cells is mechanism by which renal fibroblasts are generated. Here we show that members transforming growth factor-beta (TGF-beta)-induced cells. Unilateral ureter obstruction (UUO) a common model and subsequent tubulointerstitial fibrosis. In order examine role in fibrosis, their expression was investigated kidneys UUO mice. The miRNAs increased...
Aging is associated with a high prevalence of hypertension due to elevated susceptibility BP dietary salt, but its mechanism unknown. Serum levels Klotho, an anti-aging factor, decline age. We found that salt (HS) increased in aged mice and young heterozygous Klotho-knockout was vascular expression Wnt5a p-MYPT1, which indicate RhoA activity. Not only the Wnt inhibitor LGK974 antagonist Box5 Klotho supplementation inhibits HS-induced elevation, similarly Rho kinase fasudil, reduced p-MYPT1...
We examined whether ANG II and TNF-alpha cooperatively induce vascular inflammation using the expression of monocyte chemoattractant protein (MCP)-1 as a marker inflammation. stimulated MCP-1 in synergistic manner smooth muscle cells. II-induced was potently inhibited to nonstimulated basal level by blockade p38-dependent pathway but only partially NF-kappaB-dependent pathway. In contrast, TNF-alpha-induced suppressed NF-kappaB activation modestly p38 activation. II- NF-kappaB- pathways...
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Growth hormone and IGF-1 have been suggested to tissue-protective effects. Ghrelin is a stomach-derived growth secretagogue. The effects of ghrelin on ischemia/reperfusion-induced renal failure in mice were examined. Ischemic acute was induced by bilateral artery clamping for 45 min reperfusion 24 h. (100 microg/kg mouse) or vehicle injected subcutaneously six times before surgery three after every 8 Twenty-four hours reperfusion, the right kidney isolated perfused. Acetylcholine (ACh)-...
Maternal malnutrition, which causes prenatal exposure to excessive glucocorticoid, induces adverse metabolic programming, leading hypertension in offspring. In offspring of pregnant rats receiving a low-protein diet or dexamethasone, synthetic mRNA expression angiotensin receptor type 1a (Agtr1a) the paraventricular nucleus (PVN) hypothalamus was upregulated, concurrent with reduced DNA methyltransferase 3a (Dnmt3a), binding DNMT3a Agtr1a gene, and demethylation. Salt loading increased BP...
Subcutaneous adipose tissue contains a lot of stem cells [adipose-derived (ASCs)] that can differentiate into variety cell lineages. In this study, we isolated ASCs from Wistar rats and examined whether would efficiently vascular endothelial (ECs) in vitro. We also administered wire injury model rat femoral artery their effects. expressed CD29 CD90, but not CD34, suggesting resemble bone marrow-derived mesenchymal cells. When induced to ECs with growth medium (EGM), Flt-1, Flk-1 or mature EC...
The Ecotropic viral integration site 1 (Evi1) is a zinc finger transcription factor, which located on chromosome 3q26, over-expression in some acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS). Elevated Evi1 expression AML associated with unfavorable prognosis. Therefore, one of the strong candidate molecular target therapy for leukemia. MicroRNAs (miRNAs) are small non-coding RNAs, vital to many cell functions that negatively regulate gene by translation or inducing...
Background— It is well known that diabetes mellitus a major risk factor for vascular diseases such as atherosclerosis and restenosis after angioplasty. has become clear advanced glycation end products (AGE) their receptor (RAGE) are implicated in diseases, especially mellitus. Nevertheless, the mechanisms by which often associated with remain unclear. Methods Results— To study role of endogenous cytokines tumor necrosis factor-α (TNF-α) interleukin-6 development expression RAGE, we used...
Although the role of calcineurin-dependent pathway in development cardiac hypertrophy has been intensively studied, little is known its vascular inflammatory diseases such as atherosclerosis and restenosis after angioplasty. To help elucidate calcineurin inflammation, we infected cultured smooth muscle cells (VSMCs) with an adenovirus construct expressing a constitutively active mutant calcineurin, examined effect on expression monocyte chemoattractant protein-1 (MCP-1). We also vivo using...
Although it has been established that myocyte enhancer factor 2 (MEF2) plays pivotal roles in the development of cardiovascular system as well skeletal muscle cells, little is known its role vascular inflammatory diseases such atherosclerosis and restenosis after angioplasty. To investigate MEF2 inflammation p38 activation MEF2, we infected cultured rat smooth cells (VSMCs) with an adenovirus construct expressing a dominant-negative mutant MEF2A (MEF2ASA) or mitogen-activated protein kinase...
Senescent cells are metabolically active and produce a variety of proinflammatory cytokines. It was previously reported that atherosclerotic plaques contain senescent cells, suggesting senescence may contribute to the progression atherosclerosis. In this study, we induced cellular in vascular endothelial (VECs) using hydrogen peroxide (H 2 O ) or an adenovirus expresses constitutively mutant Ras (AdRas12V) studied expression Both H treatment AdRas12V infection VECs, as assessed by...
Epigenetic modulation may underlie the progression of diabetic nephropathy (DN). Involvement TGFB1 in mesangial fibrosis DN led us to hypothesize that Tgfb1 DNA demethylation contributes DN. In primary cells from (db/db) mouse kidneys, and upregulation mRNA progressed simultaneously. USF1 binding site promoter region were demethylated, increased, with decreased DNMT1 db/db compared control. Given downregulation expression by folic acid, antioxidant Tempol reversed demethylation, increased...
Objectives: It has been reported that erythropoietin protects the kidneys from ischemia/reperfusion injury. In present study, we examined role of Akt and endothelial nitric oxide synthase in protective effect on injury kidney. Methods: Erythropoietin was injected peritoneal space ICR mice after its assessed by measuring blood urea nitrogen creatinine, histological analysis. Phosphorylation western blot Endothelial gene null were also used to examine renoprotective erythropoietin. Results:...
Although identification of the target mRNAs micro RNAs (miRNAs) is essential to understanding their function, low complementarity between miRNAs and has complicated this process. In study, we sought identify which reduce expression transcription factor Zeb-2, a transcriptional repressor E-cadherin known be down regulated by members miR-200 family (miR-200a,b,c miR-429, miR-141).We first used computational predicting system 82 candidate bound 3'UTR region Zeb-2 mRNA. Of these miRNAs,...
Background: Adipose tissue-derived stem cells (ASC) produce a variety of cytokines that potentially mediate the proangiogenic and antiapoptotic effects ASC. We examined whether ASC produced angiopoietin-1 (Ang1) Ang1 functionally mediated ASC-induced suppression neointimal formation. Methods Results: production was measured by enzyme-linked immunosorbent assay. Production endogenous inhibited with small interfering RNA (siRNA) for Ang1. Overproduction achieved an adenovirus expresses...