Geobacterales is a recently proposed order comprising members who originally belonged to the well-known family Geobacteraceae, which key group in terrestrial ecosystems involved biogeochemical cycles and has been widely investigated bioelectrochemistry bioenergy fields. Previous studies have illustrated taxonomic structure of most this based on genomic phylogeny; however, several are still pendent or chaotic status owing lack genome sequences. To address issue, we performed reassignment...

In paddy soil, bacteria from the family Geobacteraceae have been shown to strongly contribute biogeochemical cycle. However, no species with validly published names isolated soil. this study, we and characterized four novel ferric reducing in soils of three different fields Japan. The strains, S43T, Red53T, S62T, Red111T, were Gram-stain negative, strictly anaerobic, chemoheterotrophic, motile peritrichous flagella. Phylogenetic studies based on 16S rRNA gene sequences, five concatenated...

A photochemical reaction mediated by light-activated molecules (photosensitizers) in photodynamic therapy (PDT) causes molecular oxygen to be converted into highly reactive species (ROS) that are beneficial for cancer therapy. As the active consumer and primary regulator of apoptosis, mitochondria known as an important target optimizing PDT outcomes. However, most clinically used photosensitizers exhibited a poor tumor accumulation profile well lack targeting ability. Therefore, applying...

Cetylpyridinium chloride (CPC), a quaternary ammonium compound, which is present in mouthwash, effective against bacteria, fungi, and enveloped viruses. This study was conducted to explore the antiviral effect of CPC on SARS-CoV-2. There are few reports wild-type SARS-CoV-2 at low concentrations such as 0.001%-0.005% (10-50 µg/mL). Interestingly, we found that suppressed infectivity human isolated strains (Wuhan, Alpha, Beta, Gamma) even saliva. Furthermore, demonstrated shows...

Abstract The cell adhesion in a multiwalled carbon nanotube‐coated collagen sponge (MWCNT‐coated sponge) was investigated. Immediately after seeding, the cells adhered to inner surface of MWCNT‐coated and significantly larger number were observed there than for pure used as control. On sponge, appeared favorable spread early stages center part which rarely attached without MWCNT‐coating. It suggested that physical structure MWCNTs effective initial from result serum‐free culture....

Bacteria of the family Geobacteraceae are particularly common and deeply involved in many biogeochemical processes terrestrial freshwater environments. As part a study to understand cycling sediments, three iron-reducing isolates, designated as Red96T, Red100T, Red88T, were isolated from soils two paddy fields pond sediment located Japan. The cells Gram-negative, strictly anaerobic, rod-shaped, motile, red-pigmented on agar plates. Growth these strains was coupled reduction Fe(III)-NTA,...

Abstract: Antimicrobial photodynamic therapy (aPDT) has beneficial effects in dental treatment. We applied captopril-protected gold (Au 25 (Capt) 18 ) clusters as a novel photosensitizer for aPDT. Photoexcited Au under light irradiation generated singlet oxygen ( 1 O 2 ). Accordingly, the antimicrobial and cytotoxic of blue light-emitting diode (LED) were evaluated. generation LED (420–460 nm) was detected by methotrexate (MTX) probe. The photoexcited (0, 5, 50, 500 µg/mL) on oral bacterial...

Carbon nanohorns have been used to enhance the rate of differentiaton mesenchymal stem cells into obsteoblasts<italic>via</italic>macrophage activation.

Three bacterial strains, designated Red330 T , Red736 and Red745 were isolated from forest paddy soils in Japan. Strains are flagella-harbouring strictly anaerobic bacteria forming red colonies. A 16S rRNA gene sequence-based phylogenetic tree showed that all three strains located a cluster, including the type of Geomonas species, which recently separated genus Geobacter within family Geobacteraceae . Similarities sequences among oryzae S43 species 96.3–98.5 %. The genome-related indexes,...

Antibacterial photodynamic therapy (aPDT) utilizes reactive oxygen species such as singlet (1O2) and free radicals via photosensitizers, which are light light-sensitive agents, to reduce bacterial infections. It has been utilized a treatment for dental diseases in place of antibiotic therapies. However, aPDT does not always cause the desired therapeutic effect due instability organic photosensitizers formation biofilms. To promote antibacterial antibiofilm effects aPDT, we have proposed...

A marine strain, designated KK4T, was isolated from the surface of a starfish, Patiria pectinifera, which collected seawater off coast Hokkaido, Japan. Strain KK4T is Gram-stain-negative, non-spore-forming, rod-shaped, aerobic bacterium that forms yellow-pigmented colonies. phylogenetic relationship analysis, based on 16S rRNA gene sequences, revealed strain closely related to Ulvibacter marinus IMCC12008T, antarcticus IMCC3101T and litoralis KMM 3912T, with similarities 96.9, 95.8 95.6 %,...

Abstract The surface topography of a material can influence osteoclast activity. However, the structural factors that promote activity have not yet been investigated in detail. Therefore, we osteoclastogenesis by testing various defined patterns with different dimensions and shapes. systematic patterns, made cyclo-olefin polymer, were prepared at micron-, submicron-, nano-scale groove, hole, or pillar shape 1:1 pitch ratio. RAW264.7 cells cultured on these presence receptor activator NF-κB...

Carbon nanomaterials (CNMs) are used in various functional materials and products as well basic research, predominantly engineering. Because of their high biocompatibility, use CNMs bioapplications is widely anticipated. Many researchers have examined the kinetics toxicity intravenously administered CNMs. However, little research on vivo locally implanted has been reported. In this study, single-walled carbon nanotubes (SWCNTs) were between periosteum parietal bone mice. The SWCNTs observed...

Three bacterial strains (Red232T, Red267T and Red630T) were isolated from paddy soils sampled in Japan. Cells of these Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures for motility, formed red colonies on agar plates. Phylogenetic trees based 16S rRNA gene multiple single-copy sequences showed that the three a cluster type Anaeromyxobacter species, independent any other strain genera. Similarity values genomes among...

Forty-eight Acidobacteriota strains were isolated from soils and sediments in Japan. Among them, six representative strains, designated W79T, W786T, Red222T, Red802T, Red803T, Red804T, subjected to the taxonomic classification. These are Gram-stain-negative, non-spore-forming, rod-shaped, facultative anaerobic bacterium that can reduce ferric iron. Phylogenetic phylogenomic trees based on 16S rRNA genes multiple single-copy gene sequences showed Red804T formed a cluster with type of Geothrix...

Carbon nanotubes (CNTs) are potentially important biomaterials because of their chemical, physical, and biological properties. Our research indicates that CNTs exhibit high compatibility with bone tissue. The guided regeneration (GBR) technique is commonly applied to reconstruct alveolar treat peri-implant defects. In GBR, defects covered a barrier membrane prevent the entry nonosteogenic cells such as epithelial fibroblasts. also maintains space for new formation. However, mechanical...

Direct contact between bone and implant materials is required for dental implants. Titanium used the material owing to its mechanical biological properties. The anodisation as surface treatment was employed enhance osteogenesis around titanium. Moreover, carbon nanohorn (CNH), a type of nanometer-sized material, reported promote formation. Thus, it expected that if anodised Ti (AnTi) modified with CNHs, Ti-bone would be enhanced. In this study, CNHs by electrophoresis obtained titanium...

This study developed a novel antibacterial conjugate based on carbon nanohorns for peri-implantisis, an inflammatory disease around dental implants, which may result in failing implants by bone loss them.