A5071470775- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Photosynthetic Processes and Mechanisms
- Genomics and Chromatin Dynamics
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Cellular transport and secretion
- Nuclear Structure and Function
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- Protein Kinase Regulation and GTPase Signaling
- Advanced Electron Microscopy Techniques and Applications
- Glycosylation and Glycoproteins Research
- Cancer-related Molecular Pathways
- Retinal Development and Disorders
- DNA Repair Mechanisms
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Retinal Diseases and Treatments
- Calpain Protease Function and Regulation
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Connexins and lens biology
- Mitochondrial Function and Pathology
- PI3K/AKT/mTOR signaling in cancer
MRC Laboratory of Molecular Biology
2016-2025
Shenzhen University
2025
Nanjing Medical University
2024
Jiangsu Province Hospital
2024
Huazhong University of Science and Technology
2019-2023
Tongji Hospital
2019-2023
The Francis Crick Institute
2022-2023
Jinan University
2021-2023
Guangzhou Medical University
2022-2023
Hunan University of Traditional Chinese Medicine
2022-2023
ABCB10 is one of the three ATP-binding cassette (ABC) transporters found in inner membrane mitochondria. In mammals essential for erythropoiesis, and protection mitochondria against oxidative stress. therefore a potential therapeutic target diseases which increased mitochondrial reactive oxygen species production stress play major role. The crystal structure apo-ABCB10 shows classic exporter fold ABC transporter structure, an open-inwards conformation, ready to bind substrate or nucleotide...
Posttranslational modifications add tremendous complexity to proteomes; however, gaps remain in knowledge regarding the function and regulatory mechanism of newly discovered lysine acylation modifications. Here, we compared a panel non-histone patterns metastasis models clinical samples, focused on 2-hydroxyisobutyrylation (Khib) due its significant upregulation cancer metastases. By integration systemic Khib proteome profiling 20 paired primary esophageal tumor metastatic tissues with...
Abstract N6-methyladenosine (m6A) methylation is an abundant modification in eukaryotic mRNAs. Accumulating evidence suggests a role for RNA m6A various aspects of cancer biology. In this study, we aimed to explore the biological tumor metastasis and identify novel therapeutic strategies esophageal squamous cell carcinoma (ESCC). Integration genome-wide CRISPR/Cas9 functional screening with highly invasive metastatic ESCC subline models led identification METTL3, catalytic subunit...
Kinetochores assemble onto specialized centromeric CENP-A (centromere protein A) nucleosomes (CENP-A Nuc ) to mediate attachments between chromosomes and the mitotic spindle. We describe cryo–electron microscopy structures of human inner kinetochore constitutive centromere associated network (CCAN) complex bound reconstituted α-satellite DNA. CCAN forms edge-on contacts with , a linker DNA segment repeat emerges from fully wrapped end nucleosome thread through central CENP-LN channel that...
Abstract Faithful chromosome segregation requires robust, load-bearing attachments of chromosomes to the mitotic spindle, a function accomplished by large macromolecular complexes termed kinetochores. In most eukaryotes, constitutive centromere-associated network (CCAN) complex inner kinetochore recruits centromeres ten-subunit outer KMN that comprises KNL1C, MIS12C and NDC80C complexes. The directly attaches CCAN microtubules through NDC80C. Here, we determined high-resolution cryo-EM...
Activation of the Cyclin B/Cdc2 kinase complex triggers entry into mitosis in all eukaryotic cells. B1 localization changes dramatically during cell cycle, precipitously transiting from cytoplasm to nucleus at beginning mitosis. Presumably, this relocalization promotes phosphorylation nuclear targets critical for chromatin condensation and envelope breakdown. We show here that previously characterized cytoplasmic retention sequence B1, responsible its interphase localization, is actually an...
Reversible phosphorylation of nuclear proteins is required for both DNA replication and entry into mitosis. Consequently, most cyclin-dependent kinase (Cdk)/cyclin complexes are localized to the nucleus when active. Although our understanding transport processes has been greatly enhanced by recent identification targeting sequences soluble import factors with which they interact, mechanisms used target Cdk/cyclin remain obscure; this in part because these lack obvious localization sequences....
Abstract Endonuclease G (ENDOG), a mitochondrial nuclease, is known to participate in many cellular processes, including apoptosis and paternal elimination, while its role autophagy remains unclear. Here, we report that ENDOG released from mitochondria promotes during starvation, which find be evolutionally conserved across species by performing experiments human cell lines, mice, Drosophila C. elegans . Under Glycogen synthase kinase 3 beta-mediated phosphorylation of at Thr-128 Ser-288...
The point centromere of budding yeast specifies assembly the large kinetochore complex to mediate chromatid segregation. Kinetochores comprise centromere-associated inner (CCAN) and microtubule-binding outer KNL1-MIS12-NDC80 (KMN) network. also contains DNA binding centromere-binding factor 1 (CBF1) CBF3 complexes. We determined cryo–electron microscopy structure assembled onto centromere-specific protein A nucleosomes (CENP-A Nuc ). This revealed a central CENP-A with extensively unwrapped...
Abstract Hepatocellular carcinoma (HCC) is one of the most common malignant tumors. Identification underlying mechanism HCC progression and exploration new therapeutic drugs are urgently needed. Here, a compound library consisting 419 FDA-approved was taken to screen potential anticancer drugs. A series functional assays showed that desloratadine, an antiallergic drug, can repress proliferation in cell lines, cell-derived xenograft (CDX), patient-derived organoid (PDO) (PDX) models....
Activation of Rho guanosine triphosphatases (GTPases) to the guanine triphosphate (GTP)-bound state is a critical event in their regulation cytoskeleton and cell signaling. Members DOCK family nucleotide exchange factors (GEFs) are important activators GTPases, but mechanism activation by catalytic DHR2 domain unknown. Through structural analysis DOCK9-Cdc42 complexes, we identify sensor within alpha10 helix that contributes release diphosphate (GDP) then discharge activated GTP-bound Cdc42....
DOCK (dedicator of cytokinesis) guanine nucleotide exchange factors (GEFs) activate the Rho-family GTPases Rac and Cdc42 to control cell migration, morphogenesis, phagocytosis. The A B subfamilies Rac, whereas D subfamily activates Cdc42. Nucleotide is catalyzed by a conserved DHR2 domain (DOCKDHR2). Although molecular basis for DOCKDHR2-mediated GTPase activation has been elucidated through structures DOCK9DHR2-Cdc42 complex, determining recognition specific are unknown. To understand...
Abstract CircRNA mitochondrial tRNA translation optimization 1 (circMTO1) functions as a tumor suppressor usually and is related to the progression of many tumors, including hepatocellular carcinoma (HCC). CircMTO1 downregulated in HCC compared adjacent nontumor tissue, which may suppress by certain signal pathways. However, underlying pathway remains largely unknown. The interactions between circMTO1 miR-541-5p were predicted through bioinformatics analysis verified using pull-down...