A5031894108- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Angiogenesis and VEGF in Cancer
- Single-cell and spatial transcriptomics
- Atherosclerosis and Cardiovascular Diseases
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Platelet Disorders and Treatments
- T-cell and B-cell Immunology
- Protease and Inhibitor Mechanisms
- Cellular Mechanics and Interactions
- Spaceflight effects on biology
- Galectins and Cancer Biology
- Cell Adhesion Molecules Research
- Helicobacter pylori-related gastroenterology studies
- Microtubule and mitosis dynamics
- Immune Response and Inflammation
- Renal Diseases and Glomerulopathies
LMU Klinikum
2022-2024
Ludwig-Maximilians-Universität München
2020-2024
Centre of Experimental Medicine of the Slovak Academy of Sciences
2021
Abstract Platelet homeostasis is essential for vascular integrity and immune defence 1,2 . Although the process of platelet formation by fragmenting megakaryocytes (MKs; thrombopoiesis) has been extensively studied, cellular molecular mechanisms required to constantly replenish pool MKs their progenitor cells (megakaryopoiesis) remains unclear 3,4 Here we use intravital imaging track dynamics megakaryopoiesis over days. We identify plasmacytoid dendritic (pDCs) as homeostatic sensors that...
Plasmacytoid dendritic cells (pDCs) display an increased abundance in visceral adipose tissue (VAT) of humans with obesity. In the current study, we set out to decipher molecular mechanisms their recruitment VAT and functional relevance this process. We observed pDC numbers murine blood, liver, spleen, after feeding a high-fat diet (HFD) for 3 wk when compared standard diet. pDCs were enriched fat-associated lymphoid clusters representing highly specific regions within VAT. HFD led...
<title>Abstract</title> Background Lung cancer brain metastasis have a devastating prognosis, necessitating innovative treatment strategies. While chimeric antigen receptor (CAR) T-cells show promise in hematologic malignancies, their efficacy solid tumors, including metastasis, is limited by the immunosuppressive tumor environment. The PD-L1/PD-1 pathway inhibits CAR T-cell activity microenvironment, presenting potential target to enhance therapeutic efficacy. This study aims evaluate...
Directional cell migration and the establishment of polarity play an important role in development, wound healing, host defense. While actin polymerization provides driving force at front, microtubule network assumes a regulatory function, coordinating front protrusion rear retraction. By using Dictyostelium discoideum cells as model for amoeboid movement different 2D 3D environments, position centrosome relative to nucleus was analyzed live-cell microscopy. Our results showed that...
Background: RNase A (the bovine equivalent to human 1) and 5 (angiogenin), are two closely related ribonucleases. is described as a powerful angiogenic factor. Whether shares the same characteristic, or interferes with vessel growth demonstrated for arteriogenesis, has never been investigated topic of this present study. Methods Results: To investigate whether shows pro- anti-angiogenic effect, we employed murine hindlimb model, in which femoral artery ligation (FAL) results arteriogenesis...
Helicobacter pylori (H. pylori) is a bacterial pathogen that exclusively colonizes the human gastric mucosa and can cause persistent infection. In this process, H. employs various strategies to avoid recognition by immune system. These range from passive defense (e.g., altered LPS or flagellin structures) prevent pattern receptors (PRRs) more active approaches, such as inhibition of IL-2 secretion proliferation T cells via VacA. Despite growing evidence actively manipulates system for its...
Abstract Platelet homeostasis is essential for vascular integrity and immune defense. While the process of platelet formation by fragmenting megakaryocytes (thrombopoiesis) has been extensively studied, cellular molecular mechanisms required to constantly replenish pool their progenitor cells (megakaryopoiesis) remains unclear. Here we use intravital 2 photon microscopy track individual megakaryopoiesis over days. We identify plasmacytoid dendritic (pDCs) as crucial bone marrow niche that...
Arteriogenesis strongly depends on leukocyte and platelet recruitment to the perivascular space of growing collateral vessels. The standard approach for analyzing arteries leukocytes in arteriogenesis is ex vivo (immuno-) histological methodology. However, this technique does not allow measurement dynamic processes such as blood flow, shear stress, cell-cell interactions, particle velocity. This paper presents a protocol monitor during utilizing intravital imaging. method described here...
Arteriogenesis strongly depends on leukocyte and platelet recruitment to the perivascular space of growing collateral vessels. The standard approach for analyzing arteries leukocytes in arteriogenesis is ex vivo (immuno-) histological methodology. However, this technique does not allow measurement dynamic processes such as blood flow, shear stress, cell-cell interactions, particle velocity. This paper presents a protocol monitor during utilizing intravital imaging. method described here...