A5075829336- Platelet Disorders and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cardiac Fibrosis and Remodeling
- Atherosclerosis and Cardiovascular Diseases
- Blood Coagulation and Thrombosis Mechanisms
- Heparin-Induced Thrombocytopenia and Thrombosis
- Tissue Engineering and Regenerative Medicine
- Intramuscular injections and effects
- Cardiac Structural Anomalies and Repair
- Cell Adhesion Molecules Research
- Acute Ischemic Stroke Management
- Immune cells in cancer
- Phagocytosis and Immune Regulation
- High Altitude and Hypoxia
- Acute Myocardial Infarction Research
- Protease and Inhibitor Mechanisms
- Coronary Interventions and Diagnostics
- Venous Thromboembolism Diagnosis and Management
- Chemokine receptors and signaling
- Thermoregulation and physiological responses
- Cardiovascular Health and Disease Prevention
- Adipokines, Inflammation, and Metabolic Diseases
- Blood Pressure and Hypertension Studies
- Physiological and biochemical adaptations
- Neuroinflammation and Neurodegeneration Mechanisms
Ludwig-Maximilians-Universität München
2010-2024
LMU Klinikum
2010-2024
German Centre for Cardiovascular Research
2015-2023
Heartland Alliance
2020
Kursk State Medical University
2018
Deutsches Herzzentrum München
2012
BackgroundStent thrombosis (ST) is a rare but serious complication following percutaneous coronary intervention. Analysis of thrombus composition from patients undergoing catheter thrombectomy may provide important insights into the pathological processes leading to formation. We performed large-scale multicentre study evaluate specimens in with ST across Europe.
Leukocyte-released antimicrobial peptides contribute to pathogen elimination and activation of the immune system. Their role in thrombosis is incompletely understood. Here we show that cathelicidin LL-37 abundant thrombi from patients with acute myocardial infarction. Its mouse homologue, CRAMP, present arterial following vascular injury, derives mainly circulating neutrophils. Absence hematopoietic CRAMP bone marrow chimeric mice reduces platelet recruitment thrombus formation. Both induce...
To investigate whether immune cell composition and content of neutrophil extracellular traps (NETs) in relation to clinical outcome are different between acute ischemic stroke (AIS) myocardial infarction (AMI), we performed histologic analysis correlated results with procedural parameters.We retrieved thrombi from patients AIS (n = 71) AMI 72) during endovascular arterial recanalization analyzed their NET by immunohistology. We then associated thrombus parameters cardiac function AMI....
Neutrophils provide a critical line of defense in immune responses to various pathogens, inflicting self-damage upon transition hyperactivated, procoagulant state. Recent work has highlighted proinflammatory neutrophil phenotypes contributing lung injury and acute respiratory distress syndrome (ARDS) patients with coronavirus disease 2019 (COVID-19). Here, we use state-of-the art mass spectrometry–based proteomics transcriptomic correlative analyses as well functional vitro vivo studies...
Abstract Arterial macrophages have different developmental origins, but the association of macrophage ontogeny with their phenotypes and functions in adulthood is still unclear. Here, we combine fate-mapping analysis single-cell RNA sequencing to establish cellular identity during homeostasis, response angiotensin-II (AngII)-induced arterial inflammation. Yolk sac erythro-myeloid progenitors (EMP) contribute substantially adventitial give rise a defined cluster resident immune cells...
Vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are based on a range of novel platforms, with adenovirus-based approaches (like ChAdOx1 nCov-19) being one them. Recently, complication SARS-CoV-2-targeted adenovirus vaccines has emerged: immune thrombocytopenia, either isolated, or accompanied by thrombosis (then termed VITT). This is characterized low platelet counts, and in the case VITT, also platelet-activating factor 4 antibodies reminiscent heparin-induced...
Venous thromboembolism (VTE) comprising deep venous thrombosis and pulmonary embolism is a major cause of morbidity mortality. Short-term immobility-related conditions are risk factor for the development VTE. Paradoxically, long-term immobilized free-ranging hibernating brown bears paralyzed spinal cord injury (SCI) patients protected from We aimed to identify mechanisms immobility-associated VTE protection in cross-species approach. Mass spectrometry-based proteomics revealed an...
Catheter-based renal artery denervation has been successfully introduced as alternative treatment for patients suffering from drug-resistant essential hypertension. However, the local morphological changes within vessel wall accompanying this technique remain elusive and we sought to characterize these by utilizing simplicity radiofrequency catheter approach.Following of seven pigs, arteries were assigned either acute (n = 6), subacute (10-day follow-up, n 6) or control (untreated, 2) group....
Aims Medical treatment of arterial thrombosis is mainly directed against platelets and coagulation factors, can lead to bleeding complications. Novel antithrombotic therapies targeting immune cells neutrophil extracellular traps (NETs) are currently being investigated in animals. We addressed whether cell composition thrombi induced mouse models resemble those human patients with acute myocardial infarction (AMI). Methods results In a prospective cohort study suffering from AMI, 81 were...
Abstract Impairment of vascular integrity is a hallmark inflammatory diseases. We recently reported that single immune-responsive platelets migrate and reposition themselves to sites injury prevent bleeding. However, it remains unclear how preserve once encountering endothelial breaches. Here we demonstrate by intravital microscopy combined with genetic mouse models procoagulant activation (PA) subsequent recruitment the coagulation cascade are crucial for prevention Using novel...
Maladaptive, non-resolving inflammation contributes to chronic inflammatory diseases such as atherosclerosis. Because macrophages remove necrotic cells, defective macrophage programs can promote with persistent tissue injury. Here, we investigated the mechanisms sustaining vascular macrophages. Intravital imaging revealed a spatiotemporal niche across beds alongside mural cells (MCs)-pericytes and smooth muscle cells. Single-cell transcriptomics, co-culture, genetic deletion experiments...
Abstract Platelet homeostasis is essential for vascular integrity and immune defence 1,2 . Although the process of platelet formation by fragmenting megakaryocytes (MKs; thrombopoiesis) has been extensively studied, cellular molecular mechanisms required to constantly replenish pool MKs their progenitor cells (megakaryopoiesis) remains unclear 3,4 Here we use intravital imaging track dynamics megakaryopoiesis over days. We identify plasmacytoid dendritic (pDCs) as homeostatic sensors that...
Summary paragraph Vaccines against SARS-CoV-2 are based on a range of novel vaccine platforms, with adenovirus-based approaches (like ChAdOx1 nCov-19) being one them. Recently rare and complication targeted adenovirus vaccines has emerged: thrombosis thrombocytopenia syndrome (TTS). TTS is characterized by low platelet counts, clot formation at unusual anatomic sites platelet-activating PF4-polyanion antibodies reminiscent heparin-induced thrombocytopenia. Here, we employ in vitro vivo...
Resident cardiac macrophages have been demonstrated to facilitate the electrical conduction in heart. The physiologic heart rhythm is initiated by impulses generated sinoatrial node (SAN) and then conducted ventricles via atrioventricular (AVN). To further study role of resident system, a proper isolation from SAN AVN necessary, but it remains challenging. Here, we provide protocol for reliable microdissection murine hearts followed culture macrophages. Both, which located at junction crista...
АннотацияС использованием общенаучных и экономико-статистических методов исследования в статье рас смотрены возможности современной противотромботической фармакотерапии больных с инфарк том мозга (ишемическим инсультом) условиях стационара.Проведен сравнительный анализ ас сортимента антитромботических лекарственных средств на фармацевтическом рынке России г.Воронежа.Проанализирована фактическая структура врачебных назначений данной группы пре паратов госпитальном этапе учетом рекомендаций...
Retinoid X receptors (RXRs), as members of the steroid/thyroid hormone superfamily nuclear receptors, are crucial regulators immune response during health and disease. RXR subtype expression is dependent on tissue cell type, RXRα being relevant isoform in monocytes macrophages. Previous studies have assessed different functions RXRs positive implications agonists outcomes after ischemic injuries been described. However, impact a reduced Rxrα mononuclear phagocytes cardiac remodeling...
Resident cardiac macrophages have been demonstrated to facilitate the electrical conduction in heart. The physiologic heart rhythm is initiated by impulses generated sinoatrial node (SAN) and then conducted ventricles via atrioventricular (AVN). To further study role of resident system, a proper isolation from SAN AVN necessary, but it remains challenging. Here, we provide protocol for reliable microdissection murine hearts followed culture macrophages. Both, which located at junction crista...