A5012158066- Antimicrobial Peptides and Activities
- Oral microbiology and periodontitis research
- Dermatology and Skin Diseases
- Immune Response and Inflammation
- Advancements in Transdermal Drug Delivery
- Sphingolipid Metabolism and Signaling
- Immunotherapy and Immune Responses
- Inflammatory Bowel Disease
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Streptococcal Infections and Treatments
- Essential Oils and Antimicrobial Activity
- Glycosylation and Glycoproteins Research
- Pharmacological Effects of Natural Compounds
- Gastrointestinal Bleeding Diagnosis and Treatment
- Antifungal resistance and susceptibility
- Bone Metabolism and Diseases
- Cancer Immunotherapy and Biomarkers
- Gastrointestinal Tumor Research and Treatment
- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Chemokine receptors and signaling
- Effects and risks of endocrine disrupting chemicals
- MicroRNA in disease regulation
- Lipid Membrane Structure and Behavior
Waldorf College
2017-2022
Hospital Alemán
2014-2020
University of Iowa
2011-2018
British Society of Periodontology
2018
University of Illinois Chicago
2018
Eppendorf (Germany)
2014
Heidelberg University
2011
Hadassah Medical Center
2007
Uppsala University
2006
ABSTRACT There is growing evidence that the role of lipids in innate immunity more important than previously realized. How interact with bacteria to achieve a level protection, however, still poorly understood. To begin address mechanisms antibacterial activity, we determined MICs and minimum bactericidal concentrations (MBCs) common skin oral cavity—the sphingoid bases d -sphingosine, phytosphingosine, dihydrosphingosine fatty acids sapienic acid lauric acid—against four Gram-negative seven...
The present study shows that polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs) can interact enhance developmental neurobehavioral defects when the exposure occurs during a critical stage of neonatal brain development. PBDEs are used in large quantities as flame-retardant additives polymers, especially manufacture great variety electrical appliances, textiles. In contrast to well-known persistent compounds PCBs DDT, have been found increase environment human mother's...
MicroRNAs (miRs) regulate inflammation and BMP antagonists, thus they have potential uses as therapeutic reagents. However, the molecular function of miR-200c in modulating proinflammatory bone metabolic mediators osteogenic differentiation is not known. After was transduced into a human embryonic palatal mesenchyme (HEPM) (a cell line preosteoblasts), using lentiviral vectors, resulting overexpression increased biomarkers, including osteocalcin (OCN) transcripts calcium content. expression...
Sphingoid bases found in the outer layers of skin exhibit antimicrobial activity against gram-positive and gram-negative bacteria. We investigated uptake several sphingoid by <i>Escherichia coli</i> <i>Staphylococcus aureus</i>, assessed subsequent ultrastructural damage. <i>E. <i>S. aureus</i> were incubated with <smlcap>d</smlcap>-sphingosine, dihydrosphingosine, or phytosphingosine at ten times their MIC for 0.5 4 h, respectively, to...
Oral mucosal and salivary lipids exhibit potent antimicrobial activity for a variety of Gram-positive Gram-negative bacteria; however, little is known about their spectrum or mechanisms action against oral bacteria. In this study, we examine the two fatty acids three sphingoid bases Porphyromonas gingivalis, an important colonizer cavity implicated in periodontitis. Minimal inhibitory concentrations, minimal bactericidal kill kinetics revealed variable, but potent, P. indicating that lipid...
In the skin, aging is associated with overall epidermal thinning, decreased barrier function, and gradual deterioration of immune response. However, presence role cytokines, chemokines, biologic analytes (CCBAs) in immunosenescence are not known. Here we identified age-related changes skin properties CCBAs from stratum corneum healthy human subjects, providing a means to utilize as benchmarks for health. Transepidermal water loss a(*) (skin redness) an age-dependent manner, were...
Background: Recent studies point to the clinical utility of using peri‐implant sulcular fluid (PISF) as a valuable diagnostic aid for monitoring tissue health. The objectives this study are determine levels key biomarkers in PISF periodontal maintenance participants and compare them with their corresponding gingival crevicular (GCF) obtained from same participants. Methods: GCF were collected an implant contralateral natural tooth after examination 73 interleukin (IL)‐1α, IL‐1β, IL‐6, IL‐8,...
Histatins are human salivary gland peptides with anti-microbial and anti-inflammatory activities. In this study, we hypothesized that histatin 5 binds to Porphyromonas gingivalis hemagglutinin B (HagB) attenuates HagB-induced chemokine responses in myeloid dendritic cells. Histatin bound immobilized HagB a surface plasmon resonance (SPR) spectroscopy-based biosensor system. SPR spectroscopy kinetic equilibrium analyses, protein microarray studies I-TASSER structural modeling all demonstrated...
Abstract Purpose Several complications may arise in patients wearing complete prosthetic appliances, including denture‐associated infections and mucosal stomatitis due to Candida species. This study evaluated the activity of anti‐ agents denture adhesive cytotoxicities these preparations for primary human gingival epithelial (GE) keratinocytes. Materials Methods The activities antimicrobial peptides, lipids, antifungal against C. albicans ATCC 64124 or HMV4C were assessed microdilution...
Matrix metalloproteinases (MMPs) are enzymes involved in periodontal tissue destruction. Hemagglutinin B (HagB) from the pathogen Porphyromonas gingivalis induces an elevated MMP response dendritic cells, but responses cultures of single-cell types do not reflect local environment. The objective this study was to measure HagB-induced a transwell co-culture system containing gingival epithelial (GE) keratinocytes, and CD4+ T-cells. Transwell co-cultures were assembled treated with or without...
The underlying causes of denture stomatitis may be related to the long-term use adhesives, which predispose individuals oral candidiasis. In this study, we hypothesize that antimicrobial peptides and antifungal antibiotics have diminished anti-Candida activities in adhesive. To show this, nine five with without 1.0% adhesive were incubated Candida albicans strains ATCC 64124 HMV4C radial diffusion assays. gels adhesive, HNP-1, HBD2, HBD3, IP-10, LL37 (only one strain), histatin 5...
CXCL10 (IP-10) is a small 10 kDa chemokine with antimicrobial activity. It induced by IFN-γ, chemoattracts mononuclear cells, and promotes adhesion of T cells. Recently, we detected on the surface skin in oral cavity. In current study, used broth microdilution radial diffusion assays to show that inhibits growth Escherichia coli, Staphylococcus aureus, Corynebacterium jeikeium, striatum, Candida albicans HMV4C, but not bovis, Streptococcus mutans, mitis, sanguinis, Fusobacterium nucleatum,...
Abstract Individual computational models of single myeloid, lymphoid, epithelial, and cancer cells were created combined into multi-cell used to predict the collective chemokine, cytokine, cellular biomarker profiles often seen in inflamed or cancerous tissues. Predicted chemokine cytokine output from gingival epithelial keratinocytes (GE KER), dendritic (DC), helper T lymphocytes (HTL) exposed lipopolysaccharide (LPS) synthetic triacylated lipopeptide (Pam3CSK4) as well multiple myeloma...
Lipids endogenous to skin and mucosal surfaces exhibit potent antimicrobial activity against Porphyromonas gingivalis, an important colonizer of the oral cavity implicated in periodontitis. Our previous work demonstrated fatty acid sapienic (C(16:1Δ6)) P. gingivalis found that treatment alters both protein lipid composition from those controls. In this study, we further examined whole-cell differences between acid-treated bacteria untreated controls, utilized open-source functional...