A5064654921- Monoclonal and Polyclonal Antibodies Research
- HER2/EGFR in Cancer Research
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Pancreatic and Hepatic Oncology Research
- Protein Kinase Regulation and GTPase Signaling
- Caveolin-1 and cellular processes
- Radiopharmaceutical Chemistry and Applications
- Cancer-related gene regulation
- Toxin Mechanisms and Immunotoxins
- PI3K/AKT/mTOR signaling in cancer
- Bone and Joint Diseases
- RNA Research and Splicing
- Metabolism, Diabetes, and Cancer
- Telomeres, Telomerase, and Senescence
- Cancer-related Molecular Pathways
- Liver Disease Diagnosis and Treatment
- Genomic variations and chromosomal abnormalities
- Peptidase Inhibition and Analysis
- Chronic Lymphocytic Leukemia Research
- Ubiquitin and proteasome pathways
- Bone health and treatments
- Blood groups and transfusion
- Cancer Diagnosis and Treatment
- RNA modifications and cancer
National Institutes of Health
1999-2006
National Cancer Institute
1999-2001
Georgetown University
2001
Tokyo Metropolitan Komagome Hospital
1990
Background: Most (70%–100%) ovarian carcinomas express high levels of the epidermal growth factor receptor (EGFR). To examine relationship between EGFR and invasive phenotype, we assessed integrin expression, adhesion, matrix metalloproteinase (MMP) activity, migration in cancer cells which expression was modified. Methods: NIH:OVCAR-8 human carcinoma were transfected with an vector containing complementary DNA antisense orientation (EGFR-antisense cells) or alone (vector control cells). We...
Identification of tumor‐derived proteins in the circulation may allow for early detection cancer and evaluation therapeutic responses. To identify circulating proteins, mice were immunized with concentrated culture medium conditioned by human breast cells. Antibodies generated hybridomas screened against media from both normal epithelial cells tumor Antibody selectively reacting cell–conditioned was further characterized. This led to development a monoclonal antibody (Alper‐p280) that reacts...
Over-expression of epidermal growth factor receptor (EGFR) in ovarian cancer has been well documented. Human NIH:OVCAR-8 carcinoma cells were transfected with an expression vector containing the anti-sense orientation truncated human EGFR cDNA. over-expression resulted decreased protein and mRNA expression, cell proliferation tumor formation nude mice. In accordance reduced levels anti-sense-expressing cells, tyrosine phosphorylation was compared to untransfected parental treated EGF....
Abstract Coexpression of epidermal growth factor receptor (EGFR) and c-erbB-2 in 47–68% ovarian cancer cells indicate their strong association with tumor formation. We examined the effects simultaneous antisense- or immunosuppression EGFR expression on invasive phenotype, aneuploidy, genotype cultured human carcinoma (NIH:OVCAR-8). report here that suppression both results regression aneuploidy genomic imbalances NIH:OVCAR-8 cells, restores a more normal gene profile. Combined cytogenetic...
We assessed the relationship between parathyroid hormone-related protein (PTHrP) and development of humoral hypercalcemia malignancy (i.e., due to production by solid tumors hy-percalcemic factors) assaying tumor extracts from hypercalcemic normocalcemic patients with cancer for immunoreactive PTHrP contents. Immunoreactive was demonstrated in 21 22 tissues obtained malignancy. rarely seen tissue 34 two severe bone metas-tases. Gel filtration studies revealed a molecular-size heterogeneity...
Abstract Introduction Immortalization is a key step in malignant transformation, but immortalization alone insufficient for transformation. Human mammary epithelial cell (HMEC) transformation complex process that requires additional genetic changes beyond and can be accomplished vitro by accumulation of expression H-ras. Methods HMEC were immortalized serial passaging transduction with the catalytic subunit human telomerase gene ( hTERT ). The cells passaged studied combination G- banding...
With the aim of developing an effective cancer immunotherapy for common epithelial cancer, a new class bifunctional antibody (BFA) was developed; one arm this BFA recognized c‐ erb B‐2 gene product, and other CD3ɛ, T‐cell specific surface antigen. Application with human peripheral blood lymphocytes exhibited anti‐tumor activity in vitro on breast tumor cell line, ZR‐75–1, which expressed abundant erbB ‐2 product its surface. These results indicate that recognizing oncogene is potential agent...
Abstract Background: Glia maturation factor beta (GMF-beta) is a growth and differentiation of brain cells, stimulation neural degeneration inhibition proliferation tumor cells. It was recently found to involve in the progression serous ovarian carcinoma. In present study, we generated novel antibody against human GMF-beta investigated potential as biomarker breast cancer. Methods findings: We monoclonal (mAb), AB-GMF-beta using hybridoma technology native protein an immunogen. This mAb IgG1...
Pancreatic cancer (PC) and diabetes mellitus (DM) represent major research involvement worldwide.In the last few years, several clinical studies have confirmed that DM progression has a key role in development of PC.In this mini-review, we analyze current epidemiological data, addition to basic structural physiological properties pancreatic tissue, relationship cellular mechanisms between subtypes PC.We discuss findings, as well offer perspectives on proteomics biomarker research.
<div>Abstract<p>Coexpression of epidermal growth factor receptor (EGFR) and c-erbB-2 in 47–68% ovarian cancer cells indicate their strong association with tumor formation. We examined the effects simultaneous antisense- or immunosuppression EGFR expression on invasive phenotype, aneuploidy, genotype cultured human carcinoma (NIH:OVCAR-8). report here that suppression both results regression aneuploidy genomic imbalances NIH:OVCAR-8 cells, restores a more normal gene profile....
<div>Abstract<p>Coexpression of epidermal growth factor receptor (EGFR) and c-erbB-2 in 47–68% ovarian cancer cells indicate their strong association with tumor formation. We examined the effects simultaneous antisense- or immunosuppression EGFR expression on invasive phenotype, aneuploidy, genotype cultured human carcinoma (NIH:OVCAR-8). report here that suppression both results regression aneuploidy genomic imbalances NIH:OVCAR-8 cells, restores a more normal gene profile....
Abstract Background: Amplification of the HER2 gene and/or overexpression its protein product have been reported in 25% to 30% breast carcinomas and associated with poorer outcomes. However, there are still highly controversial aspects testing. In present study, we generated a novel monoclonal antibody against human native epitope investigated clinical potential managing carcinoma. Methods findings: We (mAb), AB-HER2 using hybridoma technology folding as an immunogen. This mAb is IgG1...
Abstract Background: Poly(rC)-binding protein-1 (PCBP-1) is a nucleic-acid-binding protein that localizes in nucleus and/or cytoplasm of cells. Shuttling between and has been related to its function regulation RNA transcription, translation also implicated involvement cancer metastasis, progression chemoresistance. In the present study, we investigated levels expression PCBP-1 breast carcinoma potential use as biomarker differential diagnosis. Methods/Results: We generated novel monoclonal...
Abstract Background: Actinin-4 is an actin cross-linked protein. The expression and subcellular distribution of actinin-4 (ACTN4) has been associated with multiple roles in tumorigenicity cancer metastasis. In the present study, we developed a novel antibody against human investigated clinical utility as biomarker metastatic breast cancer. Methods findings: We generated monoclonal (AB-Actinin-4) using hybridoma technology native ACTN4 protein immunogen. specifically recognized both denatured...