A5006892940- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- RNA Interference and Gene Delivery
- Malaria Research and Control
- Mosquito-borne diseases and control
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Viral gastroenteritis research and epidemiology
- Immune Response and Inflammation
- Vaccine Coverage and Hesitancy
- HIV Research and Treatment
- Peptidase Inhibition and Analysis
- Virus-based gene therapy research
- Phagocytosis and Immune Regulation
- Food Allergy and Anaphylaxis Research
- Cancer-related gene regulation
- Chronic Myeloid Leukemia Treatments
- Cardiac tumors and thrombi
- Complement system in diseases
- NF-κB Signaling Pathways
- Allergic Rhinitis and Sensitization
- SARS-CoV-2 and COVID-19 Research
- Transgenic Plants and Applications
- Advanced Biosensing Techniques and Applications
- Insect symbiosis and bacterial influences
National Institutes of Health
2009-2024
National Institute of Allergy and Infectious Diseases
2011-2024
National Cancer Institute
2001-2023
Center for Cancer Research
2007-2023
National Institute on Drug Abuse
2016
Technikon (Austria)
2015
The University of Tokyo
2011
ActionAid
2010
Walter Reed Army Institute of Research
1996-2010
The University of Texas Health Science Center at Houston
2007
Abstract B-cell functions in antitumor immunity are not well understood. In this study, we evaluated the role of B cells development using Friend murine leukemia virus gag-expressing mouse EL-4 (EL-4 gag), D5 melanoma, or MCA304 sarcoma cells. To screen tumors for susceptibility to B-cell-deficient immune environments, spleen from naive C57BL/6 [wild-type (WT)] and knockout (BKO) mice were cultured with irradiated tumor vitro. When stimulated gag (but tumors), IFN-γ production CD8 T natural...
On November 7th and 8th, 2022, The National Institute of Allergy Infectious Diseases (NIAID), part the Institutes Health (NIH), Coalition for Epidemic Preparedness Innovation (CEPI), Bill & Melinda Gates Foundation (BMGF), Biomedical Advanced Research Development Authority (BARDA), Wellcome Trust hosted a virtual workshop entitled "Mucosal Vaccines SARS-CoV-2: Scientific Gaps Opportunities." During workshop, researchers vaccine developers from around world discussed potential mucosal...
A new langerin + DC subset has recently been identified in murine dermis (langerin dDC), but the lineage and functional relationships between these cells epidermal Langerhans (LC) are incompletely characterized. Selective expression of cell adhesion molecule EpCAM by LC allowed viable to be easily distinguished from dDC skin lymphoid tissue ex vivo as well. Differential revealed presence at least 3 distinct subsets. We determined that exhibit different migratory capabilities vitro repopulate...
MFG-E8 (also called lactadherin and SED1) is a secreted glycoprotein that has been previously implicated in enhancement of vascular endothelial growth factor-dependent angiogenesis. Major sources vivo precise mechanisms action remain undetermined. The objective this study was to identify important further elucidate the role(s) regulation angiogenesis.We used knockout mice anti-MFG-E8 antibodies function vivo. In melanomas retinas with oxygen-induced retinopathy, colocalized pericytes rather...
Abstract CD4+ T cells play a central role in the induction and persistence of CD8+ several models autoimmune infectious disease. To improve efficacy synthetic peptide vaccine based on self-Ag, gp100, we sought to provide Ag-specific cell help. identify gp100 epitope restricted by MHC class II allele with highest prevalence patients malignant melanoma (HLA-DRB1*0401), immunized mice transgenic for chimeric human-mouse molecule (DR4-IE) recombinant human protein. We then searched reactivity...
Abstract The circumsporozoite protein (CSP) is a target for effector Ab and cell mediated immunity against malaria parasites; DNA vaccination can induce both types of response. immunogenicity efficacy two plasmids expressing different amounts Plasmodium berghei CSP were evaluated by immunizing BALB/c mice i.m. or epidermally varying the number immunizations (one to three doses) interval between immunizations. Expanding gave strongest effect, increasing antibody boosting, and, in case...
Abstract The relevance of angiogenesis in tumor biology and as a therapeutic target is well established. MFG-E8 (also termed lactadherin) developmental endothelial locus 1 (Del1) constitute two-gene family αvβ3/β5 ligands that regulate angiogenesis. After detecting mRNA murine cell lines, we sought to determine if influenced tumorigenesis Rip1-Tag2 transgenic mice, cancer model which critical. protein were increased angiogenic islets tumors mice compared with normal pancreas. Frequencies...
Seventeen malaria-naive volunteers received a recombinant Plasmodium falciparum vaccine (RLF) containing the carboxy- and amino-terminal of circumsporozoite protein (CSP) antigen without central tetrapeptide repeats. The was formulated in liposomes with either low or high dose 3-deacylated monophosphoryl lipid A (MPL) administered alum by intramuscular injection. Both formulations were well tolerated immunogenic. MPL increased sporozoite antibody titers measured ELISA, Western blot,...
Identification of tumor‐derived proteins in the circulation may allow for early detection cancer and evaluation therapeutic responses. To identify circulating proteins, mice were immunized with concentrated culture medium conditioned by human breast cells. Antibodies generated hybridomas screened against media from both normal epithelial cells tumor Antibody selectively reacting cell–conditioned was further characterized. This led to development a monoclonal antibody (Alper‐p280) that reacts...
ABSTRACT The circumsporozoite protein (CSP) from the surface of sporozoite stage Plasmodium sp. malaria parasites is among most important vaccine candidates. Gene gun injection genetic vaccines encoding berghei CSP induces a significant protective effect against challenge; however, intramuscular does not. In present study we compared immune responses and effects induced by P. delivered intradermally with needle or epidermally gene gun. Mice were immunized three times at 4-week intervals...