A5004538854- Trypanosoma species research and implications
- Radiopharmaceutical Chemistry and Applications
- Research on Leishmaniasis Studies
- Epigenetics and DNA Methylation
- Synthesis and Biological Evaluation
- Genetics and Neurodevelopmental Disorders
- Cancer-related gene regulation
- Neuroendocrine Tumor Research Advances
- Lung Cancer Research Studies
- Biochemical and Molecular Research
- Monoclonal and Polyclonal Antibodies Research
- Protein Kinase Regulation and GTPase Signaling
- Signaling Pathways in Disease
- Ubiquitin and proteasome pathways
- Viral Infections and Vectors
- Phosphodiesterase function and regulation
- Phytochemical compounds biological activities
- Adipose Tissue and Metabolism
- Chemical Reactions and Isotopes
- Botanical Studies and Applications
- Galectins and Cancer Biology
- Social Media in Health Education
- Viral Infections and Outbreaks Research
- Herpesvirus Infections and Treatments
- Head and Neck Anomalies
Consejo Nacional de Investigaciones Científicas y Técnicas
2010-2023
University of Pittsburgh
2006-2023
Fundación Ciencias Exactas y Naturales
2002-2023
University of Buenos Aires
2002-2023
Instituto de Química y Fisicoquímica Biológicas
2020-2023
University Medical Center Hamburg-Eppendorf
2010
Universität Hamburg
2010
Czech Academy of Sciences
2010
Czech Academy of Sciences, Institute of Biophysics
2010
Vanderbilt University Medical Center
2010
OBJECTIVE Chronic exercise and obesity both increase intramyocellular triglycerides (IMTGs) despite having opposing effects on insulin sensitivity. We hypothesized that chronically exercise-trained muscle would be characterized by lower skeletal diacylglycerols (DAGs) ceramides higher IMTGs account for its also the expression of key proteins involved in lipid droplet hydrolysis, DAG formation, fatty-acid partitioning oxidation associated with lipotoxic phenotype. RESEARCH DESIGN AND METHODS...
Inflammatory processes and foam cell formation are key determinants in the initiation progression of atherosclerosis. Electrophilic nitro-fatty acids, byproducts nitric oxide- nitrite-dependent redox reactions unsaturated fatty exhibit antiinflammatory signaling actions inflammatory vascular model systems. The vivo action acids chronic such as atherosclerosis remains to be elucidated.Herein, we demonstrate that subcutaneously administered 9- 10-nitro-octadecenoic acid (nitro-oleic acid)...
Thyroid cancer is the most common type of endocrine malignancy, encompassing tumors with various levels invasive growth and aggressiveness. Rap1GAP, a Rap1 GTPase-activating protein, inhibits RAS superfamily protein by facilitating hydrolysis GTP to GDP. In this study, we analyzed 197 thyroid tumor samples showed that Rap1GAP was frequently lost or downregulated in types tumors, particularly aggressive forms cancer. The downregulation due promoter hypermethylation and/or loss heterozygosity,...
Rap1b has been implicated in the transduction of cAMP mitogenic response. Agonists that increase intracellular rapidly activate (i.e. GTP binding) and phosphorylate on Ser(179) at its C terminus. cAMP-dependent protein kinase (PKA)-mediated phosphorylation is required for mitogenesis, tumorigenesis, inhibition AKT activity. However, role still remains unknown. In this study, we utilized amide hydrogen/deuterium exchange mass spectroscopy (DXMS) to assess potential conformational changes...
This work was designed to compare sentinel lymph node (SLN) uptake of 99mTc-labelled human serum albumin colloid (99mTc-HSAC), antimony sulphur (99mTc-SC) and a dextran 70 solution (99mTc-Dx) their selectivity in the identification this right rear footpad (RRF) normal mice tumour bearing mice. Radiopharmaceutical SLN (popliteal node) lumbar (LLN), second lymphatic station from RRF, were measured at different time points post-intradermal or intratumoural injection into RRF NIH Balb/c...
Abstract The large subunit of the eukaryotic ribosome possesses a long and protruding stalk formed by ribosomal P proteins. This structure is involved in translation step protein synthesis through interaction with elongation factor 2 (EF‐2). Trypanosoma cruzi complex composed four proteins about 11 kDa, TcP1α, TcP1β, TcP2α, TcP2β fifth TcP0 34 kDa. In previous work, yeast two‐hybrid (Y2H) protein–protein map T. was generated. order to gain new insight into assembly stalk, complete generated...
The current chemotherapy of Chagas disease needs to be urgently improved. With this aim, a series 16 hybrids Cinchona alkaloids and bile acids were prepared by functionalization at position C-2 the quinoline nucleus radical attack norcholane substituent via Barton-Zard decarboxylation reaction. antitrypanosomal activity was tested on different stages strains T. cruzi. In particular, eight out presented an IC50 ≤1 μg/mL against trypomastigotes CL Brener strain and/or selectivity index higher...
We report the synthesis of 16 new compounds obtained from kokusaginine and flindersiamine, main alkaloids isolated bark Balfourodendron riedelianum. The activity against axenic cultures Trypanosoma cruzi epimastigtotes trypomastigotes, as well intracellular amastigotes, is described, together with their cytotoxic three different human cell lines. synthetic strategy for preparation was based on reactivity at C4 position furoquinoline core towards nucleophiles. derivatives were synthesized by...
The aim of this work was to synthesize, label and evaluate in vivo [131I]N-(2-diethylaminoethyl)-3-iodo-4-methoxybenzamide ([131I]IMBA) as a radiotracer for B16-F0 melanoma cells, C57 mice bearing subcutaneous tumour experimentally induced lung metastases. average radio-iodination yield achieved, after labelling Sep-Pak purification, 65%, with radiochemical purity ≥96%. Biodistribution studies using [131I]IMBA (2.2 GBq/μmol) showed high specific uptake, low non-target tissue background, due...
In our search for new safe antiparasitic agents, an enzymatic pathway was applied to synthesize a series of N-pyridinylmethyl amides derived from structurally different carboxylic acids. Thirty derivatives, including 11 compounds, were prepared through lipase-catalyzed acylation in excellent yields. order optimize the synthetic methodology, impact reaction parameters analyzed. Some compounds evaluated as antiproliferative agents against Trypanosoma cruzi, parasite responsible American...
Trypanosoma cruzi cAMP-mediated invasion has long been described, however, the detailed mechanism of action pathway activated by this cyclic nucleotide still remains unknown. We have recently demonstrated a crucial role for Epac in host cell. In work, we gathered evidence indicating that cAMP/Epac is different cells lines. accordance, data collected from pull-down experiments designed to identify only active form Rap1b (Rap1b-GTP), and infection assays using transfected with constitutively...
Although the two drugs currently available for treatment of Chagas disease, Benznidazole and Nifurtimox, have proven to be effective in acute phase 60–90-day leads high toxicity unwanted side effects, presenting, addition, a low efficacy chronic disease. For this reason, new therapies that are more needed. In regard, we recently shown inhibition Epac-Rap1b pathway suppressed cAMP-mediated host cell invasion by Trypanosoma cruzi . Interestingly, it has been described vitexin, natural flavone...
The clinical potential of 111In and 90Y labelled 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid conjugated Tyr3-octreotide (DOTA-TOC) have been reported in a number publications, Phase II trials 90Y-DOTA-TOC are currently progress. However, to date, only summary the large-scale preparation these radiopharmaceuticals has published. This publication aims describe our experience small-scale synthesis DOTA-TOC hope that this will assist others other similar radioconjugates. DOTA form...
Matrix metalloproteinases (MMPs) not only play a relevant role in homeostatic processes but are also involved several pathological mechanisms associated with infectious diseases. As their clinical relevance Chagas disease has recently been highlighted, we studied the modulation of circulating MMPs by Trypanosoma cruzi infection. We found that virulent parasites from Discrete Typing Units (DTU) VI induced higher proMMP-2 and MMP-2 activity blood, whereas both low (DTU I) high virulence...