Significance New antifungal compounds are needed due to an increasing incidence of invasive fungal infections and resistance many currently used drugs. Here we show that cyanobacteria a rich source such as glycosylated lipopeptides, called hassallidins, which commonly produced by filamentous nitrogen-fixing cyanobacteria. A diverse group hassallidins their complex nonribosomal biosynthesis were characterized in detail. Hassallidins previously unidentified biosynthetic enzymes offer new...

Cyanobacteria are photosynthetic prokaryotes found in a range of environments. They infamous for the production toxins, as well bioactive compounds, which exhibit anticancer, antimicrobial and protease inhibition activities. produce broad antifungals belonging to structural classes, such peptides, polyketides alkaloids. Here, we tested cyanobacteria from wide variety environments antifungal activity. The potent macrolide scytophycin was detected Anabaena sp. HAN21/1, cf. cylindrica PH133,...

ABSTRACT Mycosporine‐like amino acids are water‐soluble secondary metabolites that protect photosynthetic microorganisms from ultraviolet radiation. Here, we present direct evidence for the production of these compounds in surface scums cyanobacteria along Baltic Sea coast. We collected 59 environmental samples southern coast Finland during summers 2021 and 2022 analysed them using high‐resolution liquid chromatography‐mass spectrometry. Our results revealed presence microbial sunscreens...

ABSTRACT Mesophotic coral ecosystems (MCEs) host a diverse array of sponge species, which represent promising source bioactive compounds. Increasing evidence suggests that sponge‐associated bacteria may be the primary producers these However, cultivating under laboratory conditions remains significant challenge. To investigate rich resource compounds synthesised by mesophotic bacteria, we retrieved 429 metagenome‐assembled genomes (MAGs) from 15 sponges, revealing strong correlation between...

Anabaenopeptins are a diverse group of cyclic peptides, which contain an unusual ureido linkage. Namalides shorter structural homologues anabaenopeptins, also The biosynthetic origins namalides unknown despite strong resemblance to anabaenopeptins. Here, we show the cyanobacterium Nostoc sp. CENA543 strain producing new (nostamide B-E (2, 4, 5, and 6)) known variants anabaenopeptins (schizopeptin 791 (1) anabaenopeptin 807 (3)). Surprisingly, produced namalide B (8) D (7), E (9), F (10) in...

Cyanobacteria are photosynthetic organisms that produce a large diversity of natural products with interesting bioactivities for biotechnological and pharmaceutical applications. Cyanobacterial extracts exhibit toxicity towards other microorganisms cancer cells and, therefore, represent source potentially novel drug discovery. We tested 62 cyanobacterial strains isolated from various Brazilian biomes antileukemic antimicrobial activities. Extracts 39 induced selective apoptosis in acute...

Nostoc is cyanobacterial genus, common in soils and a prolific producer of natural products. This research project aimed to explore Brazilian cyanobacteria for new bioactive compounds their characterization. Here we report the production hepatotoxins protease inhibitors from benthic sp. CENA543 isolated small, shallow, saline-alkaline lake Nhecolândia, Pantanal wetland area Brazil. produces exceptionally high amounts nodularin-R. first free-living found that produce nodularin at comparable...

Several cyanobacterial genera produce toxic secondary metabolites, the most well-known of which are hepatotoxic microcystins (MCYSTs). Microcystin analyses in drinking water a requirement Health Ministry (Regulation 518/2004) Brazil, but this regulation does not establish extraction and analytical method should be used; toxin quantification is usually carried out by ELISA (enzyme-linked immunosorbent assay) or HPLC (high performance liquid chromatography), efficiency depends on used. In work...

Abstract Background Many important toxins and antibiotics are produced by non-ribosomal biosynthetic pathways. Microcystins a chemically diverse family of potent peptide the end-products hybrid NRPS PKS secondary metabolic pathway. They variety cyanobacteria responsible for poisoning humans as well deaths wild domestic animals around world. The chemical diversity microcystin is attributed to number genetic events that have resulted in diversification pathway assembly. Results Here, we show...

Significance Cyclodextrins are cyclic oligosaccharides used in the pharmaceutical industry to improve drug delivery. Here, we show that cyclodextrins antifungal activity of anabaenolysins A and B lipopeptides both produced by same cyanobacteria. This study identifies putative biosynthetic gene cluster involved synthesis these unique cyanobacterial lipopeptide anabaenolysins.

Microcystins are a family of chemically diverse hepatotoxins produced by distantly related cyanobacteria and potent inhibitors eukaryotic protein phosphatases 1 2A. Here we provide evidence for the biosynthesis rare variants microcystin that contain selection homo-amino acids benthic cyanobacterium Phormidium sp. LP904c. This strain produces at least 16 chemical many which homophenylalanine or homotyrosine. We retrieved complete 54.2 kb (mcy) gene cluster from draft genome assembly. Analysis...

In this study, we investigated forty cyanobacterial isolates from biofilms, gastropods, brackish water and symbiotic lichen habitats. Their aqueous organic extracts were used to screen for apoptosis-inducing activity against acute myeloid leukemia cells. A total of 28 showed cytotoxicity rat (IPC-81) The design the made it possible eliminate known toxins, such as microcystins nodularin, or metabolites with anti-leukemic activity, adenosine its analogs. test on human embryonic kidney...

Lichens have been widely used in traditional medicine, especially by indigenous communities worldwide. However, their slow growth and difficulties the isolation of lichen symbionts associated microbes hindered pharmaceutical utilisation lichen-produced compounds. Advances high-throughput sequencing techniques now permit detailed investigations complex microbial formed fungi, green algae, cyanobacteria, other bacteria within thalli. Here, we amplicon sequencing, shotgun metagenomics, silico...

Abstract The human gut microbiome composition has been linked to Parkinson’s disease (PD). However, knowledge of the microbiota on genome level is still limited. Here we performed deep metagenomic sequencing and binning build metagenome-assembled genomes (MAGs) from 136 fecal microbiomes (68 PD samples 68 control samples). We constructed 952 non-redundant high-quality MAGs compared them between groups. Among these MAGs, there were 22 different Collinsella Prevotella , indicating high...

Toxic benthic mats of cyanobacteria are associated with water quality problems and animal poisonings around the world. A strain filamentous cyanobacterial genus Kamptonema was isolated from a bloom in Baltic Sea four decades ago later shown to produce cylindrospermopsins. However, exact habitat this remains unclear cylindrospermopsins have not yet been reported blooms Sea. Here, we report isolation sp. UHCC 0994 microbial mat collected shallow on coast Helsinki. We obtained draft genome...

Abstract The human gut microbiome composition has been linked to Parkinson’s disease (PD). However, knowledge of the microbiota on genome level is still limited. Here we performed deep metagenomic sequencing and binning build metagenome-assembled genomes (MAG) from 136 fecal microbiomes (68 PD samples 68 control samples). We constructed 952 non-redundant high-quality MAGs compared them between groups. Among these MAGs, there were 22 different versions Collinsella Prevotella indicating high...

The presence of cyanobacteria in water for drinking and recreational purposes is considered problematic due to their bloom-forming toxigenic potential. While most cyanotoxins are produced by extensively studied cyanobacteria, little known about other abundant genera not usually assessed toxic Here, a new isolate Limnothrix redekei from an Italian reservoir was cultured investigated using biological, chemical, genomic methods. Compared spp., the yielded high biomass productivity mainly...

Abstract The aging population worldwide is on the rise, leading to a higher number of Parkinson’s disease (PD) cases each year. PD presently second most prevalent neurodegenerative disease, affecting an estimated 7-10 million individuals globally. This research aimed identify mobile genetic elements in human fecal samples using shotgun metagenomics approach. We found over 44,000 plasmid contigs and compared populations between patients (n = 68) healthy controls 68). Significant associations...