The plant life cycle involves an alternation of generations between sporophyte and gametophyte. Currently, the genes pathways involved in gametophytic development function flowering plants remain largely unknown. A large-scale mutant screen Ds transposon insertion lines was employed to identify 130 mutants Arabidopsis thaliana with defects female gametophyte function. wide variety phenotypes were observed, ranging from different stages early embryo sac apparently normal sacs, but exhibiting...

In Angiosperms, the male gametes are delivered to female through maternal reproductive tissue by pollen tube. Upon arrival, tube releases two sperm cells, permitting double fertilization take place. Although critical role of gametophyte in reception has been demonstrated, underlying mechanisms remain poorly understood. Here, we describe lorelei, an Arabidopsis thaliana mutant impaired cell release, reminiscent feronia/sirène mutant. Pollen tubes reaching lorelei embryo sacs frequently do not...

During embryogenesis a single cell gives rise to functional multicellular organism. In higher plants, as in many other systems, essential architectural features, such body axes and major tissue layers are established early serve positional framework for subsequent pattern elaboration. Arabidopsis, the apicalbasal axis radial of tissues wrapped around it already recognizable young embryos only about hundred cells size. This axial seems provide coordinate system embryonic initiation shoot...

Abstract All species of the genus Populus (poplar, aspen) are dioecious, suggesting an ancient origin this trait. Despite some empirical counter examples, theory suggests that nonrecombining sex‐linked regions should quickly spread, eventually becoming heteromorphic chromosomes. In contrast, we show using whole‐genome scans sex‐associated region in trichocarpa is small and much younger than age genus. This indicates sex determination highly labile poplar, consistent with recent evidence...

We have previously demonstrated the production of tumor necrosis factor alpha (TNF) and interleukin-6 (IL-6) by alveolar macrophages (AM) from allergic asthmatics developing a late asthmatic reaction after bronchial allergen challenge. In order to explain modalities this monokine synthesis, we tested in vitro effect an IgE-dependent stimulation on blood monocytes (BM) AM control subjects. TNF IL-6 secretions were evaluated 24-h supernatants radioimmunoassay 7TD1 cell proliferation test,...

In yeast and animals, the anaphase-promoting complex or cyclosome (APC/C) is an essential ubiquitin protein ligase that regulates mitotic progression exit by controlling stability of cell cycle regulatory proteins, such as securin cyclins. plants, function, regulation, substrates APC/C are poorly understood. To gain more insight into roles plant APC/C, we characterized at molecular level one its subunits, APC2, which encoded a single-copy gene in Arabidopsis. We show Arabidopsis able to...

Aims: This prospective study investigated the effect of genetic polymorphisms in a biotransformation enzyme (CYP3A5) and transporter protein (ABCB1) on tacrolimus (Tac) whole blood concentrations renal transplantation, more specifically peripheral mononuclear cell (PBMC) drug concentrations, after transplantation. Materials & methods: A total 96 transplant recipients were genotyped for exon 11 (1199G>A), 21 (3435C>T) 26 (2677G>T/A) ABCB1 gene intron 3 polymorphism CYP3A5 gene. Tac PBMC...

Tacrolimus (Tac) metabolism is mainly mediated by the cytochrome P450 3A (CYP3A) subfamily. Recently, it has been reported that kidney transplant recipients carrying CYP3A4*22 decrease-of-function allele require lower Tac doses and are more at risk of overexposure than CYP3A4*1/*1 patients. This effect was shown to be independent CYP3A5*3 allelic status. However, pharmacokinetic (PK) parameters assessed in previous studies were limited on single time point whole blood trough concentrations...

Tacrolimus is an immunosuppressive drug widely used in hepatic transplantation to avoid graft rejection. Its pharmacokinetics characterized by a large interindividual variability requiring the use of therapeutic monitoring daily clinical practice. Some genetic polymorphisms biotransformation enzymes or transporter proteins, such as CYP3A5 and P-glycoprotein (ABCB1), donors and/or recipients, appear important determinants Tac blood pharmacokinetics. A recent study has shown that tissue...

A LC-MSMS method for the simultaneous determination of tamoxifen, N-desmethyltamoxifen, 4-hydroxytamoxifen and endoxifen in dried blood spots samples was developed validated. The employs an ultrasound-assisted liquid extraction a reversed phase separation Acquity(®) C18 column (150×2.1 mm, 1.7 µm). Mobile mixture formic acid 0.1% (v/v) pH 2.7 acetonitrile (gradient from 60:40 to 50:50, v/v). Total analytical run time 8 min. Precision assays showed CV % lower than 10.75% accuracy range 94.5...

Tacrolimus (Tac) is a profoundly effective immunosuppressant that reduces the risk of rejection after solid organ transplantation. However, its use hampered by narrow therapeutic window along with highly variable pharmacological (pharmacokinetic [PK] and pharmacodynamic [PD]) profile. Part this variability explained genetic polymorphisms affecting metabolic pathway. The integration CYP3A4 CY3A5 genotype in tacrolimus population-based PK (PopPK) modeling approaches has been proven to...

The first part of this work presents the sequence 20 NH2 terminus residues obtained from P30, major surface Ag Toxoplasma gondii, purified by HPLC. A synthetic peptide (P30 48-67) has been prepared both in linear form and as a multiple antigenic (MAP) construct. Immunization mice rats with P30 48-67 MAP presence IFA induces high levels IgG antibodies that recognize construct ELISA, Western blots NP-40-extracted tachyzoite Ag. Because these sera are negative immunofluorescence assays whole...

Flow microfluorometry (FMF) was used to investigate the presence of cytophilic Ig (IgE or IgG) and proportion Fc receptor (Fc epsilon R gamma R)-bearing eosinophils among from 21 hypereosinophilic patients. In a large majority cases, it possible detect IgE significantly associated with serum levels. Moreover, when lung blood were compared, occupied increased on eosinophils, whereas very few cells had IgG. This work provides further evidence that is not restricted high affinity R, but can...

Journal Article Permeability of 13 different gloves to cytotoxic agents under controlled dynamic conditions Get access Pierre E. Wallemacq, Ph.D., Ph.D. EurClinChem, is Laboratory Head Clinical Chemistry Department, University Hospital St. Luc (UHSL), and Professor, Université Catholique de Louvain, Brussels, Belgium Address correspondence Dr. Wallemacq at the Toxicology, Luc, 10 Hippocrate Avenue, B-1200 (wallemacq@lbcm.ucl.ac.be). Search for other works by this author on: Oxford Academic...

IgE FcR (FcERII) on human eosinophils was characterized and compared with FcERII present B cells (CD23). Two mAb, BB10 (anti-eosinophil FcERII) 135 (anti-CD23), bound to the major component of at 45,000 50,000 Mr, both purified hypodense a cell line (WIL-2WT). The specific ligand, myeloma IgE, able bind molecules immunoprecipitated by BB10. A cross-reactivity between mAb anti-Leishmania gp63, which is "fibronectin (Fn)-like" molecule, containing L-arginine-L-glycyl-L-aspartyl (RGD)...

As a potential alternative to whole-blood tacrolimus (TAC) monitoring, sensitive and selective method was developed for quantifying this immunosuppressant in human peripheral blood mononuclear cell population (PBMCs). These cells, expected be more specific biological matrix than whole reflect pharmacological efficacy, could promising TAC therapeutic drug monitoring (TDM). The assay using liquid chromatography-tandem mass spectrometry (LC-MS/MS). PBMCs are isolated from 7 mL by centrifugation...

Objective ATP-binding cassette, subfamily B, member 1 (ABCB1) transporter, or P-glycoprotein, is an efflux protein implicated in the absorption and distribution of various compounds, including tacrolimus cyclosporine A. In vivo studies suggest association between ABCB1 1199G>A single nucleotide polymorphism (SNP) intracellular accumulation. The aim present experimental study was to clarify vitro impact coding SNP on transport activity towards both immunosuppressive drugs. Method Two...

Venlafaxine is a bicyclic antidepressant that may be associated with severe cardiotoxicity following large overdose. The purpose of this short case series to present different patterns venlafaxine-related and discuss the potential mechanisms.Between January 2010 July 2011, four patients were admitted an ICU acute left ventricular failure venlafaxine overdoses. age female ranged from 35 65 years. None them had no history cardiovascular disease. amount ingested by 3150 13500 mg...

The aims of this work were both to validate a sensitive and specific method quantify tacrolimus (TAC) in liver biopsies after hepatic transplantation evaluate the predictive value either tissue or blood TAC concentrations for rejection 146 adult patients under TAC-based immunosuppression. Trough levels monitored daily during hospital stay by immunoassay. Liver routinely performed at day 7 posttransplantation. assay was developed liquid chromatography-mass spectrometry. limit quantification 5...