A5025485690- Blood Pressure and Hypertension Studies
- MicroRNA in disease regulation
- Heart Rate Variability and Autonomic Control
- Cardiovascular Health and Disease Prevention
- Parathyroid Disorders and Treatments
- Cancer-related molecular mechanisms research
- Cardiovascular Syncope and Autonomic Disorders
- Genetic Syndromes and Imprinting
- Hemodynamic Monitoring and Therapy
- Sodium Intake and Health
- Extracellular vesicles in disease
- Molecular Biology Techniques and Applications
- RNA Research and Splicing
- SARS-CoV-2 and COVID-19 Research
- RNA modifications and cancer
- Metabolism, Diabetes, and Cancer
- Non-Invasive Vital Sign Monitoring
- Renal Transplantation Outcomes and Treatments
- Liver Disease Diagnosis and Treatment
- Pancreatic function and diabetes
- Liver Disease and Transplantation
- Analytical Chemistry and Sensors
- Chronic Kidney Disease and Diabetes
- Circular RNAs in diseases
- Hormonal Regulation and Hypertension
Hadassah Medical Center
2016-2025
Hebrew University of Jerusalem
1997-2025
Jerusalem College of Technology
2022
Weizmann Institute of Science
2016-2022
Rockefeller University
2010-2021
University Medical Center
2018-2021
National Institute of Environmental Health Sciences
2020
National Institutes of Health
2020
Sheba Medical Center
2015
Hadassah Academic College
1980-2015
Phosphate homeostasis is maintained by a counterbalance between efflux from the kidney and influx intestine bone. FGF23 bone-derived phosphaturic hormone that acts on to increase phosphate excretion suppress biosynthesis of vitamin D. signals with highest efficacy through several FGF receptors (FGFRs) bound transmembrane protein Klotho as coreceptor. Since most tissues express FGFR, expression determines target organs. Here we identify parathyroid organ for in rats. We show gland expressed 2...
The prognostic value of sleep blood pressure reported by recent studies is variable. Our aim was to examine the relationship pressure, measured 24-hour ambulatory monitoring, with all-cause mortality. We studied a cohort 3957 patients aged 55±16 (58% treated) referred for monitoring (1991–2005). Sleep, including daytime sleep, recorded diary. Linkage national population register identified 303 deaths during 27 750 person-years follow-up. Hazard ratios (HRs) mortality in Cox proportional...
We profiled microRNAs (miRNAs) in cell-free serum and plasma samples from human volunteers using deep sequencing of barcoded small RNA cDNA libraries. By introducing calibrator synthetic oligonucleotides during library preparation, we were able to calculate the total as well specific concentrations circulating miRNA. Studying trios newborn babies their parents detected placental-specific miRNA both maternal circulations quantitated relative contribution placental miRNAs pool miRNAs....
Significance Nucleic acids mediate storage and expression of genetic information. Extracellular DNA (exDNA) exRNA are traces nucleic released from cells into the extracellular environment. Their use as disease biomarkers has been limited by technical challenges in their isolation caused abundant RNA- DNA-degrading enzymes biofluids. Using protocols developed especially for biofluids, we generated plasma serum reference profiles 13 healthy volunteers over time determined effect critical...
TGF-β1 is a pleotropic growth factor that mediates glomerulosclerosis and podocyte apoptosis, hallmarks of glomerular diseases. The expression microRNA-21 (miR-21) regulated by TGF-β1, miR-21 inhibits apoptosis in cancer cells. TGF-β1–transgenic mice exhibit accelerated loss glomerulosclerosis. We determined increases rapidly cultured murine podocytes after exposure to higher kidneys than wild-type mice. miR-21–deficient showed increased proteinuria extracellular matrix deposition fewer per...
Amyotrophic lateral sclerosis (ALS) is a multifactorial lethal motor neuron disease with no known treatment. Although the basic mechanism of its degenerative pathogenesis remains poorly understood, subcellular spatial alteration in RNA metabolism thought to play key role. The nature these RNAs elusive, and comprehensive characterization axonal involved maintaining neuronal health has yet be described. Here, using cultured spinal cord (SC) neurons grown compartmented platform followed by...
Background Autosomal dominant polycystic kidney disease (ADPKD) is clinically heterogenic. Biomarkers are needed to predict prognosis and guide management. We aimed profile microRNA (miRNA) in ADPKD gain molecular insight evaluate biomarker potential. Methods Small-RNA libraries were generated from urine specimens of patients (N = 20) with chronic other etiologies (CKD, N 20). In this report, we describe the miRNA profiles baseline characteristics. For reference, also examined transcriptome...
The assembly of a biosensor for Chlamydia trachomatis based on the microgravimetric quartz crystal microbalance (QCM) analysis bacteria association to an antibody-functionalized electrode is described. sensing interfaces consist primary cystamine monolayer assembled onto Au electrodes associated with crystal. further modified sulfosuccinylimidyl 4-(p-maleimidophenyl)butyrate (sulfo-SMPB) and goat IgG−anti-mouse IgG Fc-specific Ab or fragmented F(ab')2 anti-mouse that act as sublayers...
<h3>Background</h3> Although it has been somewhat overlooked, resting heart rate is an established predictor of cardiovascular and noncardiovascular outcome. We assessed the determinants mortality associations measured during ambulatory blood pressure monitoring (ABPM) to evaluate its informativeness activity sleep. <h3>Methods</h3> studied a cohort 3957 patients aged 55 ± 16 (mean SD) years (58% treated for hypertension) who were referred ABPM 1991 2005. Heart nondipping was defined as...
Systolic blood pressure (SBP) and diastolic (DBP) frequently display a linear relationship characterized by the systolic-versus-diastolic slope ('Slope') or 'ambulatory arterial stiffness index' [AASI = 1 - (diastolic-versus-systolic Slope)] correlation coefficient r. We evaluated effect of using symmetric regression on AASI its dependence clinical characteristics 24-h ambulatory monitoring.Ambulatory monitoring data 140 patients (age 56 +/- 17 years, 45% men) were retrieved from service...
MicroRNA (miRNA) alterations accompanying interstitial fibrosis and tubular atrophy (IFTA) in kidney allografts may point toward pathologic mechanisms. Small-RNA sequencing provides information on total miRNA abundance specific expression allows analysis of differential based read counts. MiRNA profiles 8 human allograft biopsies (4 IFTA 4 normal biopsies, discovery set) were characterized using barcoded deep-sequencing a cDNA library prepared from multiplexed RNA. Statistical the sequence...
Interleukin 33 (IL-33) is among the earliest-released cytokines in response to allergens that orchestrate type 2 immunity. The prolyl cis-trans isomerase PIN1 known induce for eosinophil survival and activation by stabilizing mRNAs, but function of upstream signaling pathways asthma unknown. Here we show interleukin receptor associated kinase M (IRAK-M) a target critical IL-33 allergic asthma. NMR analysis docking simulations suggest might regulate IRAK-M conformation signaling. Upon...
Schistosomiasis traditionally has been diagnosed by detecting eggs in stool or urine. However, the sensitivity of these examinations is limited, especially travelers with a low worm burden. Serologic tests have greater sensitivity, but their results remain positive regardless treatment and thus cannot be used for follow-up patients. We hypothesized that detection microRNAs (miRNAs) serum can overcome drawbacks existing diagnostic methods.Twenty-six returning schistosomiasis (based on...
Early detection of relapsed lymphoma improves response and survival. Current tools lack power for early relapse, while being cumbersome expensive. We searched sensitive biomarkers that precede clinical serve further studies on therapy relapse. recruited 20 healthy adults, 14 diffuse large B-cell (DLBCL) patients 11 Hodgkin (HL) at diagnosis. Using small-RNA sequencing we identified in DLBCL increased plasma levels miR-124 miR-532-5p, decreased miR-425, miR-141, miR-145, miR-197, miR-345,...
It was shown that immunocompromised patients have significantly reduced immunologic responses to COVID-19 vaccines. The immunogenicity of vaccine/infection in with solid tumors is reduced. We evaluated the response and/or BNT162b2 mRNA vaccine among cancer on active treatments and reviewed previous literature identify subgroups may require third vaccination.Anti-SARS-CoV-2 S1/S2 antibodies were measured a cohort 202 treatment chemotherapy (96), (52), biologic (46), hormonal (12) for early (n...
Abstract Circulating nucleic acids, encapsulated within small extracellular vesicles (EVs), provide a remote cellular snapshot of biomarkers derived from diseased tissues, however selective isolation is critical. Current laboratory‐based purification techniques rely on the physical properties small‐EVs rather than their inherited fingerprints. We established highly‐selective assay, termed EV‐CATCHER, initially designed for high‐throughput analysis low‐abundance small‐RNA cargos by...
Most patients with chronic kidney disease develop secondary hyperparathyroidism disabling systemic complications. Calcimimetic agents are effective tools in the management of hyperparathyroidism, acting through allosteric modification calcium-sensing receptor (CaR) on parathyroid gland (PT) to decrease hormone (PTH) secretion and PT cell proliferation. This study showed that rats were fed an adenine high-phosphorus diet had increased serum PTH mRNA levels at 7 21 d. For studying effect...
OBJECTIVES: To find possible association between liver enzymes and mortality in older people. DESIGN: A prospective cohort study. SETTING: Jerusalem. PARTICIPANTS: systematically selected representative sample of 455 70‐year‐old ambulatory individuals was prospectively followed for 12 years. MEASUREMENTS: An extensive social medical profile developed at age 70 using a detailed interview physical ancillary examination. Information on obtained annually. Differences survival subjects stratified...