A5012586926- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Helicobacter pylori-related gastroenterology studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Animal testing and alternatives
- Microbial infections and disease research
- Animal Virus Infections Studies
- Molecular Biology Techniques and Applications
- Inflammatory Bowel Disease
- Diet and metabolism studies
- Veterinary medicine and infectious diseases
- Bacteriophages and microbial interactions
- Probiotics and Fermented Foods
- RNA Research and Splicing
- Gastrointestinal motility and disorders
- Parvovirus B19 Infection Studies
- Animal Genetics and Reproduction
- Zoonotic diseases and public health
- Mycobacterium research and diagnosis
- Epigenetics and DNA Methylation
- Neuroendocrine regulation and behavior
- Single-cell and spatial transcriptomics
- Cystic Fibrosis Research Advances
- Pharmacological Effects of Natural Compounds
University of Missouri
2016-2025
Missouri College
2010-2025
University of Luxembourg
2022
San Diego Zoo Institute for Conservation Research
2017
University of Missouri Hospital
2017
Ericsson (United States)
2015-2017
Saint Louis Zoo
2017
Care Resource
2015
Southern Illinois University School of Medicine
2006
University of Arizona
2002
The commensal gut microbiota has been implicated as a determinant in several human diseases and conditions. There is mounting evidence that the of laboratory mice (Mus musculus) similarly modulates phenotype mouse models used to study disease development. While differing model phenotypes have reported using purchased from different vendors, composition uniformity fecal various genetic backgrounds vendors unclear. Using culture-independent methods robust statistical analysis, we demonstrate...
Countless studies have identified differences between the gut microbiota of humans affected with myriad conditions and healthy individuals, animal models are commonly used to determine whether those causative or correlative. Recently, concerns arisen regarding reproducibility institutions across time. To influence three common husbandry-associated factors that vary institutions, groups weanling mice were placed in either static ventilated microisolator caging, aspen paperchip bedding, one...
The role of Th17 cells in type I diabetes (TID) remains largely unknown. Glutamic acid decarboxylase (GAD) sequence 206–220 (designated GAD2) represents a late-stage epitope, but GAD2-specific T cell receptor transgenic producing interferon γ (IFNγ) protect against passive TID. Because IFNγ is known to inhibit cells, effective presentation GAD2 peptide under noninflammatory conditions may TID at advanced disease stages. To test this premise, was genetically incorporated into an...
Significance Pigs have many features that make them attractive as biomedical models, especially in regenerative medicine. Here, we introduced inactivating mutations simultaneously into both alleles of the recombination activating gene ( RAG ) 2 fibroblasts derived from minipigs and then used somatic-cell nuclear transfer to produce RAG2 −/− cloned animals with a severe immune deficiency (SCID) phenotype lacking T B cells. When human induced pluripotent (iPS) cells were injected these SCID...
The laboratory mouse is the most widely used animal model for biomedical research, due in part to its well-annotated genome, wealth of genetic resources, and ability precisely manipulate genome. Despite importance genetics quality control (QC) not standardized, lack cost-effective, informative, robust platforms. Genotyping arrays are standard tools research remain an attractive alternative even era high-throughput whole-genome sequencing. Here, we describe content performance a new iteration...
Three murine helicobacter species have recently been identified: Helicobacter hepaticus, muridarum, and bilis. Infections with H. hepaticus bilis associated hepatitis hepatic neoplasia. In this study, oligonucleotide primers were designed from regions of the 16S rRNA gene that are conserved among members genus. The assay amplified expected 374-bp product all three rodent was able to detect as little 5 pg bilis, or muridarum DNA. specificity reaction determined by testing cecal DNA uninfected...
A filamentous, gram-negative, motile bacterium with a single polar sheathed flagellum was isolated from gallbladders of hamsters cholangiofibrosis and centrilobular pancreatitis. Bacteria grew under microaerophilic conditions at 37 42 degrees C, were oxidase, catalase, arginine aminopeptidase, L-arginine arylamidase positive, reduced nitrate to nitrite, resistant cephalothin, exhibited intermediate susceptibility nalidixic acid. Sequence analysis the 16S rRNA gene indicated that novel member...
Using animal models, the gut microbiota has been shown to play a critical role in health and disease of many organ systems. Unfortunately, model studies often lack reproducibility when performed at different institutions. Previous our laboratory have that mice can vary with number husbandry factors leading us speculate differing environments may alter microbiota, which turn influence phenotypes. As an extension these studies, we hypothesized shipping from mouse producer institution will...
Several associations have been made between characteristics of the resident gut microbiota and human health disease susceptibility. Animal models provide means to test these correlations prospectively evaluate causality. Experimental fecal transfer (FMT), or intentional transplantation microbes into recipient mice depleted their autochthonous with antibiotics, is a commonly used method testing relationships. The true completeness microbial through such procedures poorly documented in...
To evaluate the safety of a plasmid DNA-lipid complex, series good laboratory practice (GLP) studies were conducted with VCL-1005, DNA expression vector containing both human class I MHC HLA-B7 heavy-chain and beta 2-microglobulin (beta 2m) light-chain genes formulated cationic lipid, DMRIE/DOPE. In mice, repeated intravenous injection VCL-1005 at doses 0.1, 1.0, or 10 micrograms for 14 days had only incidental effects on clinical chemistry hematology, did not result in any organ pathology....
The Effectiveness of a Solution-Focused, Public Alternative School for Dropout Prevention and Retrieval Get access Cynthia Franklin, Franklin Search other works by this author on: Oxford Academic Google Scholar Calvin L. Streeter, Streeter Johnny S. Kim, Kim Stephen J. Tripodi Children & Schools, Volume 29, Issue 3, July 2007, Pages 133–144, https://doi.org/10.1093/cs/29.3.133 Published: 01 2007 Article history Accepted: 21 February
// Aaron C. Ericsson 1,2,3,4 , Sadia Akter 5 Marina M. Hanson 3,4 Susheel B. Busi Taybor W. Parker Rebecca J. Schehr 4 Miriam A. Hankins 1,3,4 Carin E. Ahner Justin Davis 5,6 Craig L. Franklin 2,3,4 James Amos-Landgraf and Elizabeth Bryda 1 Rat Resource Research Center, University of Missouri, Columbia, MO, USA 2 MU Metagenomics 3 Department Veterinary Pathobiology, College Medicine, Informatics Institute, 6 Health Management Informatics, Correspondence to: Bryda, email: Keywords : gut,...
TNF-α antagonists provide benefit to patients with inflammatory autoimmune disorders such as Crohn's disease, rheumatoid arthritis, and ankylosing spondylitis. However, TNF antagonism unexplainably exacerbates CNS autoimmunity, including multiple sclerosis neuromyelitis optica. The underlying mechanisms remain enigmatic. We demonstrate that TNFR2 deficiency results in female-biased spontaneous demyelination myelin oligodendrocyte glycoprotein-specific 2D2 TCR transgenic mice. Disease...
It is estimated that 1.4 million people in the United States suffer from Inflammatory Bowel Disease (IBD), with an overall annual health care cost of more than $1.7 billion. Although exact etiology this disease remains unknown, research suggests it a multifactorial associated aberrant gastrointestinal microbial populations (dysbiosis). The C57BL/6 and C3H/HeJBir mouse strains targeted mutations IL-10 gene are commonly used models to study IBD. However, anecdotally, phenotype can vary...
The gut microbiome of humans and animals is critical to host health. Mice are used investigate the its influences; however, predictive value such studies hindered by cage effects due coprophagy. Our objectives were evaluate influence density on statistical power detect treatment-dependent a selective pressure composition. C57BL/6 mice separated into groups 2 or 4 per then assigned receiving enrofloxacin, broad-spectrum antibiotics, control. Fecal samples collected at weeks 0, 1, 4, along...
To study the impact of differing specific pathogen-free gut microbiomes (GMs) on a murine model inflammatory bowel disease, selected GMs were transferred using embryo transfer (ET), cross-fostering (CF), and co-housing (CH). Prior work showed that GM method microbial composition donor recipient can influence colonization disease phenotypes in dextran sodium sulfate-induced colitis. When low richness was to with high via CH, failed successfully colonize, more severe phenotype resulted when...
Abstract Objective To determine the pharmacokinetics of extended-release buprenorphine (XRB) in adult swine. We hypothesized that after a single SC administration XRB swine, plasma concentrations would be at or above therapeutic threshold 0.1 ng/mL and not result major injection site reactions. Methods Extended-release was administered once to 2 cohorts female Yorkshire swine low (0.2 mg/kg) high doses (0.4 mg/kg). Blood collected from an indwelling jugular catheter prior for 13 total time...