Probiotics have been used to treat gastrointestinal disorders. However, the effect of orally intubated probiotics on oral disease remains unclear. We assessed potential administration Lactobacillus gasseri SBT2055 (LG2055) for Porphyromonas gingivalis infection. LG2055 treatment significantly reduced alveolar bone loss, detachment and disorganization periodontal ligament, bacterial colonization by subsequent P. challenge. Furthermore, expression secretion TNF-α IL-6 in gingival tissue was...

Short-chain fatty acids are a major by-product of anaerobic metabolism and can be detected in gingival fluid from periodontal pockets. Since most T cells present subjacent to the pocket epithelium conjunction with plasma cells, it is important know how these affected by short-chain produced subgingival plaque. The purpose this study examine effects extracellular metabolites periodontopathic bacteria on proliferation cytokine production mouse splenic as potential mechanism imbalance among...

Butyric acid, an extracellular metabolite from periodontopathic bacteria, induces apoptosis in murine and human T- B-cells, whereas intact gingival fibroblasts isolated healthy humans are resistant to butyric-acid-induced apoptosis. We examined the susceptibility of inflamed adult persons with periodontitis acid significantly suppressed viability induced a dose-dependent manner. The incubation butyric DNA fragmentation apoptotic changes such as chromatin condensation, hypodiploid nuclei,...

ABSTRACT Recently, it has been suggested that the oral administration of Porphyromonas gingivalis, a keystone pathogen for periodontal disease, induces dysbiosis mouse intestinal microbiota and affects barrier function. Since streptococci are predominant bacterial group, we compared effect their on tract to P. gingivalis. Swallowing bacteria caused gut dysbiosis, due increased Bacteroides Staphylococcus decreased Lactobacillus spp. Furthermore, infection an increase in lactate decreases...

The purpose of this study was to examine the effect butyric acid, an extracellular metabolite from periodontopathic bacteria, on apoptosis induction in murine thymocytes, splenic T cells, and human Jurkat cells. Butyric acid significantly suppressed T-cell viability both a concentration- time-dependent fashion. results DNA fragmentation assay indicated that rapidly induced thymocytes (with 1.25 mM 6 h after treatment) cells 2.5 16 treatment). Incubation or with 5 for 21 resulted typical...

Cariogenic and periodontal pathogens are thought to be etiological factors in the development of cardiovascular disease. We assessed involvement pathogen Aggregatibacter actinomycetemcomitans cariogenic Streptococcus mutans atherosclerosis apolipoprotein E-deficient spontaneously hyperlipidemic (Apoe(shl)) mice. The mice were treated intravenously with A. HK1651, S. GS-5, or phosphate-buffered saline three times a week for 3 weeks killed at 15 age. areas aortic sinus that covered...

Porphyromonas gingivalis has been shown to accelerate atherosclerotic lesion development in hyperlipidemic animals. Atherosclerosis is a disease characterized by inflammation of the arterial wall. Recent studies have suggested that NLRP3 inflammasome plays an important role vascular and atherosclerosis. Herein, we investigated possible association between atherosclerosis periodontal induced P. infection using apolipoprotein E-deficient, spontaneously (Apoeshl) mice. Oral with wild-type (WT)...

The pathogenicity of oral bacteria was studied by measuring the development subcutaneous abscesses in mice after infection with Actinomyces viscosus and Streptococcus mitis either singly or as co-aggregated pairs. Heat-treated cells were also tested. co-aggregates examined various viable heat-treated combinations two bacterial species. More formed A. than S. at all concentrations Also, abscess formation strains produced a higher percentage those caused pure suspensions mitis. Co-aggregated...

Porphyromonas gingivalis has been shown to accelerate atherosclerotic lesion development in hyperlipidemic animals. We assessed the potential of a nasal vaccine against P. infection for prevention atherosclerosis. Apolipoprotein E-deficient spontaneously (Apoe(shl)) mice were nasally immunized with 40-kDa outer membrane protein (OMP) plus cholera toxin (CT) as adjuvant and then challenged intravenously strain 381. The animals euthanized 11 or 14 weeks later. Atheromatous lesions proximal...

The purpose of this study was to determine whether epigallocatechin-3-gallate (EGCG) ameliorates Porphyromonas gingivalis-induced atherosclerosis. EGCG is a polyphenol extract from green tea with health benefits and P. gingivalis shown here accelerate atheroma formation in murine model. Apolipoprotein E knockout mice were administered or vehicle drinking water; they then fed high-fat diets injected three times week for 3 weeks. Mice killed at 15 Atherosclerotic plaques the proximal aorta...

ABSTRACT We previously reported that butyric acid, an extracellular metabolite from periodontopathic bacteria, induced apoptosis in murine thymocytes, splenic T cells, and human Jurkat cells. In this study, we examined the ability of acid to induce peripheral blood mononuclear cells (PBMC) effect bacterial lipopolysaccharide (LPS) on apoptosis. Butyric significantly inhibited anti-CD3 monoclonal antibody- concanavalin A-induced proliferative responses a dose-dependent fashion. This...

The resurgence in mycobacterial infection worldwide has led to renewed attention the pathogenesis of Mycobacterium species. Although interferon-gamma (IFN-gamma) is a principal mediator macrophage activation, macrophages infected with are poor response at cytokine. However, molecular mechanisms underlying remain unclear. purpose this study was elucidate mechanism IFN-gamma infection. Our data clearly demonstrate that due induction suppressor cytokine signal (SOCS) negative regulators...

Autoimmune responses to heat-shock protein 60 (HSP60) contribute the progression of atherosclerosis, whereas immunization with HSP60 may induce atheroprotective responses. We assessed capacity an vaccine that targeted a recombinant from Porphyromonas gingivalis (rGroEL) protective mucosal immune response. Female apolipoprotein E-deficient spontaneously hyperlipidemic (Apoe shl ) mice received sublingual delivery rGroEL prior P. 381 injection. The animals were euthanized 16 weeks later....

Recent studies have shown an association between periodontal disease and cardiovascular disease. We previously reported that intravenous challenge with Aggregatibacter actinomycetemcomitans ( Aa) accelerated atherosclerosis in apolipoprotein E-deficient spontaneously hyperlipidemic (Apoe shl ) mice. In this study, we investigated whether live cells were required for induction or lipopolysaccharide (LPS) alone was sufficient to increase atherosclerotic damage. Mice injected intravenously Aa...

It has been reported that periodontitis is associated with an increased risk of atherosclerosis. Accumulating evidence suggests endothelial dysfunction early marker for To determine how periodontal infections contribute to dysfunction, we examined the effect Porphyromonas gingivalis on human umbilical vein cells (HUVEC). P. significantly suppressed viability HUVEC, induced DNA fragmentation and annexin V staining, caspase-3, caspase-8, caspase-9 activities. also expression GADD153 GRP78...

Abstract We have previously demonstrated that butyric acid induces cytotoxicity and apoptosis of murine thymocytes, splenic T cells, human Jurkat cells. Therefore, to determine the apoptotic signaling pathway induced by acid, we investigated contribution reactive oxygen species (ROS), mitochondria, ceramide, mitogen-activated protein kinases in acid-induced cell apoptosis. After exposure cells a pronounced accumulation ROS was seen. Pretreatment with antioxidant N-acetyl-cysteine or...

ABSTRACT In this study, we demonstrated that the 40-kDa outer membrane protein of Porphyromonas gingivalis (40-kDa OMP) nasally administered with a nontoxic chimeric adjuvant combines A subunit mutant cholera toxin E112K pentameric B heat-labile enterotoxin from enterotoxigenic Escherichia coli (mCTA/LTB) elicited long-term protective immune response. Immunization OMP and mCTA/LTB induced high levels 40-kDa-OMP-specific immunoglobulin G (IgG) IgA antibodies (Abs) in sera significant...

Bacteria in the dental biofilm surrounding marginal gingival grooves cause periodontal diseases. Numerous bacteria within consume nutrients from crevicular fluid. Furthermore, some gram-negative mature biofilms produce butyrate. Thus, epithelial cells close proximity to are at risk of both starvation and exposure In present study, we determined combined effects butyrate on cell death underlying mechanisms.The Ca9-22 line was used as an vitro counterpart cells. Cell measured amount total DNA...

A cytoplasmic soluble fraction of Actinobacillus actinomycetemcomitans Y4 was isolated and characterized as suppressing mitogen-stimulated proliferation cytokine production by C3H/HeN mouse splenic T cells. This factor, designated suppressive factor 1 (SF1), from the supernatant sonicated whole bacteria purified Q-Sepharose Fast Flow column chromatography, DEAE-Sepharose hydroxyapatite high-pressure liquid chromatography (HPLC), Protein Pack 300 & 125 gel filtration HPLC. Sodium dodecyl...

Monocyte recruitment to the endothelium is a crucial step in inflammatory response that precedes development of atherosclerosis. We assessed effect Porphyromonas gingivalis on monocyte adhesion endothelial cells, cytokine production during monocyte/endothelial cell co-culture, and expression molecules human umbilical vein cells (HUVEC) their ligands monocytes. challenge significantly increased THP-1 monocytes HUVEC, interleukin (IL)-6, IL-8, chemoattractant protein 1 (MCP-1) co-cultures...