A5102005407- Circadian rhythm and melatonin
- Microtubule and mitosis dynamics
- Estrogen and related hormone effects
- Genomics and Chromatin Dynamics
- Retinoids in leukemia and cellular processes
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Skin Protection and Aging
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
- Dermatology and Skin Diseases
- Mast cells and histamine
- Pancreatic function and diabetes
- Cancer, Stress, Anesthesia, and Immune Response
- Biochemical and Structural Characterization
- Circular RNAs in diseases
- melanin and skin pigmentation
- Melanoma and MAPK Pathways
- Nuclear Receptors and Signaling
- Asthma and respiratory diseases
- Inflammatory Biomarkers in Disease Prognosis
- Light effects on plants
- Immune cells in cancer
- Protist diversity and phylogeny
University of Wisconsin–Madison
2017-2024
University of Wisconsin Carbone Cancer Center
2023-2024
Massachusetts General Hospital
2013-2023
Harvard University
2004-2023
Xuzhou Medical College
2022
Second People’s Hospital of Huai’an
2022
Tianjin University
2017-2019
Harvard University Press
2014
Brigham and Women's Hospital
2004-2010
Tulane University
2001-2010
Aurora B is a component of the chromosomal passenger complex (CPC) required for correct spindle-kinetochore attachments during chromosome segregation and cytokinesis. The chromatin factors that recruit CPC to centromeres are unknown, however. Here we show phosphorylation histone H3 threonine 3 (H3T3ph) by Haspin necessary accumulation at subunit Survivin binds directly H3T3ph. A nonbinding Survivin-D70A/D71A mutant does not support centromeric concentration, both depletion mutation diminish...
Post-translational modifications of conserved N-terminal tail residues in histones regulate many aspects chromosome activity. Thr 3 histone H3 is highly conserved, but the significance its phosphorylation unclear, and identity corresponding kinase unknown. Immunostaining with phospho-specific antibodies mammalian cells reveals mitotic prophase dephosphorylation during anaphase. Furthermore we find that haspin, a member distinctive group protein kinases present diverse eukaryotes,...
Whether coat proteins play a widespread role in endocytic recycling remains unclear. We find that ACAP1, GTPase-activating protein (GAP) for ADP-ribosylation factor (ARF) 6, is part of novel clathrin complex regulated by ARF6 two key physiological settings, stimulation-dependent integrin critical cell migration and insulin-stimulated glucose transporter type 4 (Glut4), which required homeostasis. These findings not only advance basic understanding an early mechanistic step but also shed...
Downmodulation or loss-of-function mutations of the gene encoding NOTCH1 are associated with dysfunctional squamous cell differentiation and development carcinoma (SCC) in skin internal organs. While receptor activation has been well characterized, little is known about how transcription regulated. Using bioinformatics functional screening approaches, we identified several regulators keratinocytes, factors DLX5 EGR3 estrogen β (ERβ) directly controlling its expression differentiation. ERG3...
The pineal hormone, melatonin, has been shown to inhibit the proliferation of estrogen receptor alpha (ER α )‐positive macrophage chemotactic factor (MCF)‐7 human breast cancer cells. Previous studies from other systems indicate that melatonin modulates calcium (Ca 2+ )/calmodulin (CaM) signaling pathway either by changing intracellular concentration ([Ca ] i ) via activation its G‐protein coupled membrane receptors, or through a direct interaction with CaM. In this study, although alone had...
Hypoxic pulmonary vasoconstriction underlies the development of high-altitude edema. Anecdotal observations suggest a beneficial effect garlic in preventing symptoms. To determine whether influences vasoconstriction, we assessed on pressures rats subjected to alveolar hypoxia and isolated arterial rings. Garlic gavage (100 mg/kg body wt) for 5 days resulted complete inhibition acute hypoxic compared with control group. No difference mean pressure or heart rate response was seen between...
AbstractThe fidelity of chromosome segregation during cell division is critical to maintaingenomic stability and prevent cancer birth defects. A key set kinases that regulates thisprocess has been identified characterized over the last few years, including Aurora, Poloand Nek families. Recently we proposed a little-studied kinase known as haspin newmember this important group. During mitosis phosphorylated, associates with thechromosomes, centrosomes spindle, responsible for phosphorylation...
Cohesins and their regulators are vital for normal chromosome cohesion segregation. A number of proteins have also been localized to centrosomes proposed function there. We show that RNAi-mediated depletion factors required cohesion, including haspin, Sgo1 Scc1, leads the generation multiple acentriolar centrosome-like foci disruption spindle structure in mitosis. Live-cell imaging reveals that, haspin-depleted cells, these effects occur only as defects become manifest, they require ongoing...
A diverse array of biological processes are under circadian controls. In mouse skin, ultraviolet ray (UVR)-induced apoptosis and DNA damage responses time-of-day dependent, which controlled by core clock proteins. This study investigates the roles proteins in regulating UVB human keratinocytes (HKCs). We found that messenger RNA expression brain muscle ARNT-like 1 (BMAL1) locomotor output cycles kaput (CLOCK) genes is altered low doses (5 mJ/cm2 ) immortalized HaCat HKCs cell line. Although...
RORα is a retinoid-related orphan nuclear receptor that regulates inflammation, lipid metabolism, and cellular differentiation of several non-epithelial tissues. In spite its high expression in skin epithelium, functions this tissue remain unclear. Using gain- loss-of-function approaches to alter gene human keratinocytes (HKCs), we have found transcription factor as regulator epidermal differentiation. Among the 4 isoforms, RORα4 prominently expressed by manner increases with contrast,...
The estrogen receptor (ER)‐positive MCF‐7 human breast cancer cell line has been used extensively for the study of estrogen‐responsive cancer. However, various levels responsiveness have described in different stocks cells. Because we previously shown that pineal hormone, melatonin, inhibits proliferation cells and can modulate ER expression transactivation, investigated if exhibit a differential to anti‐proliferative effects melatonin possible mechanisms involved. (M, O, H) were examined...
Low O2 pressures present in the microenvironment of epidermis control keratinocyte differentiation and epidermal barrier function through hypoxia inducible factors (HIFs) dependent gene expression. This study focuses on investigating relations retinoic acid receptor-related orphan receptor alpha (RORα) to HIF-1α keratinocytes under hypoxic conditions. The expression level RORα is significantly elevated both human murine keratinocytes. Gene silencing RORA attenuates hypoxia-stimulated genes...
Background: Melanoma is a heterogeneous malignancy that presents an immense challenge in therapeutic development. Recent approaches targeting the oncogenic MAP kinase pathways have shown tremendous improvement overall survival of patients with advanced melanoma. However, there still urgent need for identification new strategies to overcome drug resistances and improve efficacy. Haspin (Haploid Germ Cell-Specific Nuclear Protein Kinase) belongs selected group mitotic kinases required normal...
Abstract Haspin (Haploid Germ Cell‐Specific Nuclear Protein Kinase) is a serine/threonine kinase pertinent to normal mitosis progression and mitotic phosphorylation of histone H3 at threonine 3 in mammalian cells. Different classes small molecule inhibitors haspin have been developed utilized investigate its functions. We report herein that applying inhibitor CHR‐6494 or 5‐ITu the G1/S boundary could delay entry synchronized HeLa U2OS cells, respectively, following an extended G2 S phase....
This study is aimed at exploring the biological functions and related mechanism of long noncoding RNA 704 (LINC00704) in proliferation cell cycle progression nasopharyngeal carcinoma (NPC) cells. The expression LINC00704 NPC tissues cells was quantified by quantitative real-time polymerase chain reaction (qRT-PCR). After overexpressed or knocked down lines, counting kit-8 (CCK-8) assay, 5-bromo-2'-deoxyuridine flow cytometry Transwell assay were adopted to detect proliferation, progression,...
Our recent studies have shown that haspin, a protein kinase imperative for mitosis, is engaged in the interphase progression of HeLa and U2OS cancer cells. In this investigation, we employed Fucci reporter system time-lapse imaging to examine impact haspin gene silencing on cell cycle progressions at single-cell level. We found loss induced multiple defects. Specifically, S/G2 duration was greatly prolonged by depletion or inhibition synchronous Haspin asynchronous cells led similarly...