A5012853970- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Viral gastroenteritis research and epidemiology
- Catalysis and Hydrodesulfurization Studies
- Banana Cultivation and Research
- Respiratory viral infections research
- Catalysis for Biomass Conversion
- Chromosomal and Genetic Variations
- Plant Physiology and Cultivation Studies
- Bacteriophages and microbial interactions
- Metalloenzymes and iron-sulfur proteins
- Enzyme Structure and Function
- Carbon and Quantum Dots Applications
- Catalytic Alkyne Reactions
- Microbial Metabolism and Applications
- Genetics, Aging, and Longevity in Model Organisms
- Cell death mechanisms and regulation
- Sulfur-Based Synthesis Techniques
- COVID-19 impact on air quality
- Plant Gene Expression Analysis
Institute of Biophysics
2012-2024
Chinese Academy of Sciences
2013-2024
Guangzhou Institute of Geochemistry
2021-2024
University of Chinese Academy of Sciences
2013-2024
Fujian Institute of Research on the Structure of Matter
2024
Fudan University Shanghai Cancer Center
2023
Shanghai Medical College of Fudan University
2023
Harbin Veterinary Research Institute
2022
Chinese Academy of Agricultural Sciences
2022
Haikou Experimental Station
2018-2019
CRISPR-Cas adaptive immune systems function to protect bacteria from invasion by foreign genetic elements. The CRISPR-Cas9 system has been widely adopted as a powerful genome-editing tool, and phage-encoded inhibitors, known anti-CRISPRs, offer means of regulating its activity. Here, we report the crystal structures anti-CRISPR protein AcrIIC2Nme alone in complex with Nme1Cas9. We demonstrate that inhibits Cas9 through interactions positively charged bridge helix, thereby preventing sgRNA...
Abstract To understand how the RuvC catalytic domain of Class 2 Cas proteins cleaves DNA, it will be necessary to elucidate structures RuvC-containing complexes in their catalytically competent states. Cas12i2 is a type V-I CRISPR-Cas endonuclease that target dsDNA by an unknown mechanism. Here, we report Cas12i2–crRNA–DNA and Cas12i2–crRNA complex. We reveal mechanism DNA recognition cleavage Cas12i2, activation pocket induced conformational change Helical-II domain. The seed region...
AgOTf was discovered as a highly efficient catalyst in reactions of 2-alkynylbenzaldehydes, amines, and diethylphosphite, which provided facile pathway for the synthesis 2,3-disubstituted-1,2-dihydroisoquinolin-1-ylphosphonates.
Abstract Anti-CRISPR proteins are encoded by phages to inhibit the CRISPR-Cas systems of hosts. AcrIIC5 inhibits several naturally high-fidelity type II-C Cas9 enzymes, including orthologs from Neisseria meningitidis (Nme1Cas9) and Simonsiella muelleri (SmuCas9). Here, we solve structure in complex with Nme1Cas9 sgRNA. We show that adopts a novel fold mimic size charge distribution double-stranded DNA, uses its negatively charged grooves bind occlude protospacer adjacent motif (PAM) binding...
Anti-CRISPR (Acr) proteins are encoded by phages and other mobile genetic elements inhibit host CRISPR-Cas immunity using versatile strategies. AcrIIC4 is a broad-spectrum Acr that inhibits the type II-C CRISPR-Cas9 system in several species an unknown mechanism. Here, we determined series of structures Haemophilus parainfluenzae Cas9 (HpaCas9)-sgRNA complex with and/or target DNA, as well crystal structure alone. We found resides crevice between REC1 REC2 domains HpaCas9, where its...
Abstract AcrIIA15 is an anti-CRISPR (Acr) protein that inhibits Staphylococcus aureus Cas9 (SaCas9). Although previous studies suggested it has dual functions, the structural and biochemical basis for its two activities remains unclear. Here, we determined cryo-EM structure of in complex with SaCas9-sgRNA to reveal inhibitory mechanism Acr’s C-terminal domain (CTD) mimicking dsDNA block protospacer adjacent motif (PAM) recognition. For N-terminal (NTD), our crystal structures...
Abstract The electrochemical conversion of CO 2 into valuable chemicals using renewable electricity shows significant promise for achieving carbon neutrality and providing alternative energy storage solutions. However, its practical application still faces challenges, including high consumption, poor selectivity, limited stability. Here, we propose a hybrid acid/alkali electrolyzer that couples the acidic reduction reaction (CO RR) at cathode with alkaline methanol oxidation (MOR) anode....
CDC73/Parafibromin is a critical component of the Paf1 complex (PAF1C), which involved in transcriptional elongation and histone modifications. Mutations human CDC73/HRPT2 gene are associated with hyperparathyroidism-jaw tumor (HPT-JT) syndrome, an autosomal dominant disorder. CDC73/parafibromin was initially recognized as suppressor by inhibiting cell proliferation via repression cyclin D1 c-myc genes. In recent years, it has also shown oncogenic features activating canonical Wnt/β-catenin...
A series of new Smac mimetics have been designed, synthesized, and evaluated. The most potent compound 10 binds to XIAP, cIAP-1, cIAP-2 BIR3 proteins with Ki 3.9, 0.37, 0.25 nM, respectively. Compound antagonizes XIAP in a cell-free functional assay induces rapid cIAP-1 degradation cancer cells. inhibits cell growth the MDA-MB-231 line an IC50 8.9 nM.
The environmental stability of infectious viruses in the laboratory setting is crucial to transmission potential human respiratory viruses. Different experimental techniques or conditions used studies over past decades have led diverse understandings and predictions for viral infectivity atmospheric environment. In this paper, we review current knowledge on effect simulated viruses, mainly focusing influenza coronaviruses, including severe acute syndrome coronavirus 2 Middle East...
NaOH-modified dead leaves of plane trees were used as bioadsorbent to remove methylene blue (MB) from aqueous solution. Variable influencing factors, including contact time, temperature, initial MB concentration and pH studied through single-factor experiments. The results showed that the 100 mg/L, 2.5 g/L, 7, room temperature best adsorption conditions. had a high capacity for MB, its saturated extent was 203.28 mg/g, which higher than un-modified (145.62 mg/g) some other bioadsorbents....
Plant Deg5 and Deg8 are two members of the HtrA proteases, a family oligomeric serine endopeptidases that involved in variety protein quality-control processes. These proteases located thylakoid lumen participate high-light stress responses by collaborating with other chloroplast proteins. degrade photodamaged D1 photosystem II reaction centre, allowing its situ replacement. Here, crystal structures Arabidopsis thaliana (S266A) (S292A) reported at 2.6 2.0 Å resolution, respectively. The...
Mammalian reovirus (reovirus) is a multilayered, turreted member of Reoviridae characterized by transcription dsRNA genome within the innermost capsid shell. Here, we present high-resolution in situ structures transcriptase complex an intact double-layered virion, and uncoated single-layered core particles unloaded, reloaded, pre-elongation, elongation states, respectively, obtained cryo-electron microscopy sub-particle reconstructions. At template entry RNA-dependent RNA polymerase (RdRp),...
Abstract A two‐dimensional axisymmetric computational fluid dynamics (CFD) model based on a porous media and discrete population balance was established to investigate the hydrodynamics mass transfer behavior in an airlift bioreactor for hairy root culture. During culture of Echinacea purpurea , liquid gas velocity, holdup, rate, as well oxygen concentration distribution were simulated by this CFD model. Simulative results indicated that flow turbulence played dominant role growth domain The...
Abstract Recently, cichoric acid production from hairy roots of Echinacea purpurea was significantly improved by ultrasound stimulation in an airlift bioreactor. In this article, the possible mechanism on ultrasound‐intensified root culture E. bioreactor elucidated with help computational fluid dynamics (CFD) simulation, membrane permeability detection, dissolved oxygen concentration confocal laser‐scanning microscopy (LSM) observation, and phenylalanine ammonium lyase (PAL) activity...