- Identification and Quantification in Food
- Vibrio bacteria research studies
- Microbial Community Ecology and Physiology
- Spectroscopy and Chemometric Analyses
- Metabolomics and Mass Spectrometry Studies
- Vector-Borne Animal Diseases
- Hepatitis C virus research
- Viral Infections and Vectors
- Enzyme Production and Characterization
- Listeria monocytogenes in Food Safety
- Fire effects on ecosystems
Russian Scientific Research Institute Microbe
2020-2021
Kuban State Medical University
2013
The purpose of research - based on analysis the clinical-epidemiologic data, level viral load and prevalence HCV genotypes to determine current consistencies epidemiological trends development HCV-infection in area Krasnodar Territory. Patients methods. Population incidence hepatitis C Territory was studied for period from 2004 2011. Clinical manifestations disease we evaluated base a retrospective 1913 hospital records patients with chronic C. There were investigated results 19,338...
The objective is to determine the complete nucleotide sequence and conduct a phylogenetic analysis of genome variants Puumala virus isolated in Saratov region. Materials methods. samples for study were field material collected Gagarinsky (formerly Saratovsky), Engelssky, Novoburassky Khvalynsky districts region period from 2019 2022. To specifically enrich samples, used PCR developed specific primer panel. Next, resulting products sequenced fragments assembled into one each segment genome....
Objective of the study was to identify differences in production proteins typical strain and genetically altered variant V. cholerae O1, biovar El Tor. Materials methods . Natural strains M1062 (Astrakhan, 1970) M1509 (Moscow, 2010) were used as model this work. Strains cultivated on Luria Bertani agar at 37 °C. Electrophoresis performed accordance with W.K. Laemmli technique (1970), mass-spectrometric profiling – method described by A. Shevchenko et al. Results discussion....
The epidemiology, etiology, pathogenesis, clinical manifestations, diagnosis, specific treatment and prophylaxis of neonatal listeriosis are presented in the lecture.
Objective — comparative study of protein expression in typical and genetically altered Vibrio cholerae strains O1 serogroup, biovar El Tor by means proteomic analysis.Materials methods. Clinical V. strain, M106 (Astrakhan, 1970) one, M1509 (Moscow, 2012) were used as model ones. Strains cultivated LB broth (pH7.2). Then, cell exoprotein lysate fractions obtained investigated 2D electrophoresis. Different stains examined using mass spectrometry. Survivability under osmotic oxidative stresses...
The aim of this work was to study the composition and functions intracellular extracellular proteases production Vibrio cholerae strains 569B serovar Inaba M41 Ogawa using zymographic proteomic analysis. Materials methods . Samples were obtained from cell lysates by ultrasonication bacterial cells in a 9 M urea solution. protease fraction precipitated culture liquid adding 50 % trichloroacetic acid final concentration 10 incubating on ice. Lyophilized preparations proteinase K proteovibrin...