A5028135155- RNA Research and Splicing
- Fungal and yeast genetics research
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Plant and Fungal Interactions Research
- Plant Molecular Biology Research
- Cancer-related molecular mechanisms research
- Plant Reproductive Biology
- Protein Degradation and Inhibitors
- Bioenergy crop production and management
- DNA Repair Mechanisms
- Sirtuins and Resveratrol in Medicine
- Plant Gene Expression Analysis
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Metabolism, Diabetes, and Cancer
- Gene Regulatory Network Analysis
- Retinoids in leukemia and cellular processes
The Francis Crick Institute
2016-2022
Foundation for Research and Technology Hellas
2021-2022
The Honourable Society of Lincoln's Inn
2016
Institut de Biochimie et Génétique Cellulaires
2013
Centre National de la Recherche Scientifique
2013
Université de Bordeaux
2013
Cell size varies greatly between cell types, yet within a specific type and growth condition, is narrowly distributed. Why maintenance of cell-type important remains poorly understood. Here we show that growing budding yeast primary mammalian cells beyond certain impairs gene induction, cell-cycle progression, signaling. These defects are due to the inability large scale nucleic acid protein biosynthesis in accordance with volume increase, which effectively leads cytoplasm dilution. We...
Transcription of long noncoding RNAs (lncRNAs) regulates local gene expression in eukaryotes. Many examples how a single lncRNA controls the an adjacent or nearby protein-coding have been described. Here we examine regulation locus consisting two contiguous lncRNAs and master regulator for entry into yeast meiosis, IME1. We find that cluster together with several transcription factors form regulatory circuit by which IME1 its own promoter thereby promotes expression. Inhibition stimulation...
Highlights•Expression of divergent noncoding RNAs is repressed by Rap1•Rap1 prevents initiation transcription near its binding sites•Rap1 provides directionality toward productive transcriptionSummaryMany active eukaryotic gene promoters exhibit transcription, but the mechanisms restricting expression these transcripts are not well understood. Here, we demonstrate how a sequence-specific factor represses at highly expressed genes in yeast. We find that depletion Rap1 induces large fraction...
Cell fate choices are tightly controlled by the interplay between intrinsic and extrinsic signals, gene regulatory networks. In Saccharomyces cerevisiae, decision to enter into gametogenesis or sporulation is dictated mating type nutrient availability. These signals regulate expression of master regulator gametogenesis, IME1. Here we describe how nutrients control IME1 expression. We find that protein kinase A (PKA) target rapamycin complex I (TORC1) signalling mediate regulation Inhibiting...
Transcription through noncoding regions of the genome is pervasive. How these transcription events regulate gene expression remains poorly understood. Here, we report that, in S. cerevisiae, levels a region, IRT2, located upstream promoter inducer meiosis, IME1, opposing chromatin and states. At low levels, act IRT2 promotes histone exchange, delivering acetylated H3 lysine 56 to locally. The subsequent open state directs factor recruitment induces downstream repress IME1 meiotic entry....
Cell fate decisions are controlled by multiple cell-intrinsic and -extrinsic factors. In budding yeast, the decision to enter gametogenesis or sporulation is dictated nutrient availability mating type. Recently, we showed that in diploid cells harbouring opposite types (MATa MATα), protein kinase A (PKA) target of rapamycin complex I (TORC1) signalling pathways integrate at promoter master regulatory transcription factor IME1 control via (Weidberg, et al. 2016). with a single type however,...
The directionality of gene promoters-the ratio protein-coding over divergent noncoding transcription-is highly variable. How promoter is controlled remains poorly understood. Here, we show that the chromatin remodelling complex RSC and general regulatory factors (GRFs) dictate by attenuating transcription relative to transcription. At promoters are directional, depletion leads a increase in thus decrease directionality. We find has modest effect on nucleosome positioning upstream at sites...
The directionality of gene promoters - the ratio protein-coding over divergent noncoding transcription is highly variable and regulated. How promoter controlled remains poorly understood. Here, we show that chromatin remodelling complex RSC general regulatory factors (GRFs) dictate by attenuating transcription. At are directional, depletion leads to a relative increase in thus decrease directionality. We find facilitates nucleosome positioning upstream at sites These directional also...
ABSTRACT Many long noncoding RNAs (lncRNAs) act in cis through transcription-coupled chromatin alterations that drive changes local gene expression. How some -acting lncRNAs promote and others repress expression remains poorly understood. Here we report S. cerevisiae transcription levels of the lncRNA IRT2 , located upstream promoter inducer meiosis gene, regulate opposing states. Low displays enhancer RNA-like features. At these levels, promotes histone exchange delivering acetylated H3...
Summary Many active eukaryotic gene promoters exhibit divergent noncoding transcription, but the mechanisms restricting expression of these transcripts are not well understood. Here we demonstrate how a sequence-specific transcription factor represses at highly expressed genes in yeast. We find that depletion Rap1 induces large fraction regulated promoters. Specifically, prevents initiation cryptic near its binding sites, which is uncoupled from regulation protein-coding direction. further...