A5062476580- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Histone Deacetylase Inhibitors Research
- Genetics, Bioinformatics, and Biomedical Research
- Immune Cell Function and Interaction
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Chemical Reactions and Isotopes
- Interdisciplinary Research and Collaboration
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Child and Adolescent Health
- Fungal and yeast genetics research
- Herpesvirus Infections and Treatments
- Plant Genetic and Mutation Studies
- Machine Learning in Bioinformatics
- Chromatin Remodeling and Cancer
- Spacecraft and Cryogenic Technologies
- Chemical Synthesis and Reactions
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Nutrition, Genetics, and Disease
- Crystal structures of chemical compounds
- Peptidase Inhibition and Analysis
- Advanced Proteomics Techniques and Applications
Institute for Research in Immunology and Cancer
2011-2023
Université de Montréal
2011-2023
Jawaharlal Nehru Centre for Advanced Scientific Research
2010-2012
We demonstrate that at least three different promoter variant strains of HIV-1 subtype C have been gradually expanding and replacing the standard viruses in India, possibly South Africa other global regions, over past decade. The new viral contain an additional NF-κB, NF-κB-like, or RBEIII site promoter. Although acquisition is a property shared by all subtypes, acquiring NF-κB remains exclusive C. acquired κB genetically distinct, binds p50-p65 heterodimer, strengthens levels transcription...
Linker histone H1 plays an essential role in chromatin organization. Proper deposition of linker as well its removal is for dynamics and function. chaperones perform this important task during assembly other DNA-templated phenomena the cell. Our vitro data show that multifunctional chaperone NPM1 interacts with through first acidic stretch (residues 120-132). Association was also observed cells culture. exhibited remarkable activity, it able to efficiently deposit onto dinucleosomal...
The outer domain (OD) of the HIV-1 envelope glycoprotein gp120 is an important target for vaccine design as it contains a number conserved epitopes, including large fraction CD4 binding site. Attempts to OD-based immunogens in past have met with little success. We report and characterization Escherichia coli-expressed immunogen (OD(EC)), based on sequence HxBc2 strain. OD(EC)-designed lacks variable loops V1V2 V3 incorporates 11 designed mutations at interface inner domains gp120....
Abstract Protein post-translational modifications (PTMs) play important roles in the control of various biological processes including protein–protein interactions, epigenetics and cell cycle regulation. Mass spectrometry-based proteomics approaches enable comprehensive identification quantitation numerous types PTMs. However, analysis PTMs is complicated by presence indistinguishable co-eluting isomeric peptides that result composite spectra with overlapping features prevent individual...
Histone deacetylases (HDACs) catalyze the removal of acetylation marks from lysine residues on histone and nonhistone substrates. Their activity is generally associated with essential cellular processes such as transcriptional repression heterochromatin formation. Interestingly, abnormal HDACs has been reported in various types cancers, which makes them a promising therapeutic target for cancer treatment. In current study, we aim to understand mechanisms underlying function using an in-depth...
Current protocols used to extract and purify histones are notoriously tedious, especially when using yeast cells. Here, we describe the use of a simple filter-aided sample preparation approach enabling histone extraction from mammalian cells acidified ethanol, which not only improves but also inactivates histone-modifying enzymes. We show that our improved method prevents N-terminal clipping H3, an artifact frequently observed in standard protocols. Our is scalable provides efficient...