A5068148615- Cancer Cells and Metastasis
- Radiopharmaceutical Chemistry and Applications
- Estrogen and related hormone effects
- Computational Drug Discovery Methods
- Cellular Mechanics and Interactions
- Cancer, Hypoxia, and Metabolism
- Bone Tissue Engineering Materials
- Prostate Cancer Treatment and Research
- Glutathione Transferases and Polymorphisms
- Extracellular vesicles in disease
- Metabolism, Diabetes, and Cancer
- Collagen: Extraction and Characterization
- Microfluidic and Bio-sensing Technologies
- Cancer-related molecular mechanisms research
- Electrospun Nanofibers in Biomedical Applications
- Gene expression and cancer classification
- Immune cells in cancer
- Cancer Genomics and Diagnostics
- 3D Printing in Biomedical Research
- Cancer Research and Treatments
- Microtubule and mitosis dynamics
- Erythrocyte Function and Pathophysiology
- Molecular Biology Techniques and Applications
- Chemical Reactions and Isotopes
- Immunotherapy and Immune Responses
Saint Meinrad Seminary and School of Theology
2024
Cornell University
2015-2023
Vanderbilt University
2021-2023
Abstract Although intratumoral genomic heterogeneity can impede cancer research and treatment, less is known about the effects of phenotypic heterogeneities. To investigate role cell migration heterogeneities in metastasis, we phenotypically sorted metastatic breast cells into two subpopulations based on ability. typically considered to be associated with when injected orthotopically vivo, weakly migratory subpopulation metastasized significantly more than highly subpopulation. mechanism...
Diabetes mellitus is a complex metabolic disorder that associated with an increased risk of breast cancer. Despite this correlation, the interplay between tumor progression and diabetes, particularly regard to stiffening extracellular matrix, still mechanistically unclear. Here, we established murine model where hyperglycemia was induced before development. Using model, in vitro systems, patient samples, show increases growth, matrix stiffness, glycation, epithelial-mesenchymal transition...
Intratumor heterogeneity is a well-established hallmark of cancer that impedes research, diagnosis, and treatment. Previously, we phenotypically sorted human breast cells based on migratory potential. When injected into mice, highly were weakly metastatic metastatic. The purpose this study was to determine whether these interact with each other in vitro or vivo.To assess the relationship between cell migration fitness, MDA-MB-231 SUM159PT triple negative subpopulations assayed separately 1:1...
Intracellular and environmental cues result in heterogeneous cancer cell populations with different metabolic migratory behaviors. Although glucose metabolism epithelial-to-mesenchymal transition have previously been linked, we aim to understand how this relationship fuels migration. We show that while glycolysis drives single-cell migration confining microtracks, fast slow cells display sensitivities oxidative phosphorylation inhibition. Phenotypic sorting of highly weakly subpopulations...
Cancer cell migration is highly heterogeneous, and the migratory capability of cancer cells thought to be an indicator metastatic potential. It becoming clear that a does not have inherently metastasize, with weakly often found metastatic. However, mechanism through which escape from primary tumor remains unclear. Here, utilizing phenotypically sorted human breast cells, we demonstrate disseminate via communication stromal cells. While are capable single migration, rely on cell-cell...
Scaffold mechanical properties are essential in regulating the microenvironment of three-dimensional cell culture. A coupled fiber-matrix numerical model was developed this work for predicting response collagen scaffolds subjected to various levels non-enzymatic glycation and concentrations. The scaffold simulated by a Voronoi network embedded matrix. computational validated using published experimental data. Results indicate that both glycation-induced matrix stiffening fiber density, as...
Mounting evidence suggests that the immune landscape within prostate tumors influences progression, metastasis, treatment response, and patient outcomes. In this study, we investigated spatial density of innate cell populations NOD.SCID orthotopic cancer xenografts following microinjection human DU145 cells. Our laboratory has previously developed nanoscale liposomes attach to leukocytes via conjugated E-selectin (ES) kill cells TNF-related apoptosis inducing ligand (TRAIL)....
Abstract Cancer cell migration is highly heterogeneous, and the migratory capability of cancer cells thought to be an indicator metastatic potential. It becoming clear that a does not have inherently metastasize, with weakly often found metastatic. However, mechanism through which escape from primary tumor remains unclear. Here, utilizing phenotypically sorted breast cells, we demonstrate disseminate via communication stromal cells. While are capable single migration, rely on cell-cell...
<div>Abstract<p>Although intratumoral genomic heterogeneity can impede cancer research and treatment, less is known about the effects of phenotypic heterogeneities. To investigate role cell migration heterogeneities in metastasis, we phenotypically sorted metastatic breast cells into two subpopulations based on ability. Although typically considered to be associated with when injected orthotopically <i>in vivo</i>, weakly migratory subpopulation metastasized...
<p>Video of migration MDA- cells in collagen</p>
<p>Video of migration MDA+ cells in collagen</p>
<p>MDA+ cells migrating in collagen tracks</p>
<p>MDA- cells migrating in collagen tracks</p>
<p>Supplemental data and methods</p>
<p>Supplemental data and methods</p>
<p>MDA- cells migrating in collagen tracks</p>
<p>MDA+ cells migrating in collagen tracks</p>
<p>Video of migration MDA- cells in collagen</p>
<p>Video of migration MDA+ cells in collagen</p>
<div>Abstract<p>Although intratumoral genomic heterogeneity can impede cancer research and treatment, less is known about the effects of phenotypic heterogeneities. To investigate role cell migration heterogeneities in metastasis, we phenotypically sorted metastatic breast cells into two subpopulations based on ability. Although typically considered to be associated with when injected orthotopically <i>in vivo</i>, weakly migratory subpopulation metastasized...