A5053112617- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Redox biology and oxidative stress
- Cancer Research and Treatments
- Mitochondrial Function and Pathology
- Radiopharmaceutical Chemistry and Applications
- 3D Printing in Biomedical Research
- Cell death mechanisms and regulation
- Angiogenesis and VEGF in Cancer
- Cancer, Lipids, and Metabolism
- Estrogen and related hormone effects
- Computational Drug Discovery Methods
- Prostate Cancer Treatment and Research
- Endoplasmic Reticulum Stress and Disease
- Microfluidic and Bio-sensing Technologies
- Cell Adhesion Molecules Research
- Microtubule and mitosis dynamics
- Extracellular vesicles in disease
- Adipokines, Inflammation, and Metabolic Diseases
- Metal complexes synthesis and properties
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- Glutathione Transferases and Polymorphisms
- Tissue Engineering and Regenerative Medicine
- Erythrocyte Function and Pathophysiology
Vanderbilt University
2017-2024
Tianjin University
2015-2023
Shenzhen Technology University
2023
Huazhong Agricultural University
2020
Xian Yang Central Hospital
2017
Jilin University
2016
Anhui Medical University
2012
Shanxi Datong University
2008
Significance Metastasis accounts for most cancer-associated death. To metastasize, cells can move collectively where travel together as cohorts to invade surrounding tissues. However, the mechanisms by which are unclear. Utilizing a combination of in vitro, ex vivo, and silico approaches, we demonstrated that cancer cell collective invasion is regulated energetic states leader–follower cells. Leader require more energy than follower cells, forward leader consumes depletes its available...
Previous studies have demonstrated that CTCs do not travel in the bloodstream alone, but rather are accompanied by clusters of stromal cells such as cancer associated fibroblasts (CAFs). Our laboratory has confirmed presence CAFs peripheral blood prostate (PC) patients. The observation disseminate with prompts examination role CTC survival under physiological shear stress during dissemination process using a clinically relevant, three-dimensional (3D) co-culture model. In this study, we...
Vascular endothelial growth factor (VEGF) can mediate cell migration, proliferation, and angiogenesis. During cancer progression, VEGF production is often increased to stimulate the of new blood vessels supply growing tumors with additional oxygen nutrients they require. Extracellular matrix stiffening also occurs during tumor however, crosstalk between mechanics signaling remains poorly understood. Here, we show that stiffness heightens downstream response by altering receptor-2 (VEGFR-2)...
Abstract TRAIL specifically induces apoptosis in cancer cells without affecting healthy cells. However, TRAIL’s cytotoxicity was insufficient clinical trials. Circulatory-shear stress is known to sensitize TRAIL. In this study, we examine the mechanism of sensitization with goal translating it static conditions. GsMTx-4, a Piezo1 inhibitor, found reduce shear stress-related sensitization, implicating activation as potential TRAIL-sensitizer. The agonist Yoda1 recreated stress-induced under A...
ABSTRACT Tumor vasculature is known to be more permeable than the found in healthy tissue, which turn can lead a aggressive tumor phenotype and impair drug delivery into tumors. While stiffening of stroma surrounding solid tumors has been reported increase vascular permeability, mechanism this process remains unclear. Here, we utilize an vitro model stiffening, ex ovo culture, mouse investigate molecular by matrix alters endothelial barrier function. Our data indicate that increased...
Abstract Although intratumoral genomic heterogeneity can impede cancer research and treatment, less is known about the effects of phenotypic heterogeneities. To investigate role cell migration heterogeneities in metastasis, we phenotypically sorted metastatic breast cells into two subpopulations based on ability. typically considered to be associated with when injected orthotopically vivo, weakly migratory subpopulation metastasized significantly more than highly subpopulation. mechanism...
Altered tissue mechanics and metabolism have gained significant attention as drivers of tumorigenesis, mechanoresponsive has been implicated in migration metastasis. However, heterogeneity cell populations makes it difficult to link changes behavior with metabolism, individual behaviors are not necessarily reflected population-based measurements. As such, the impact increased collagen deposition, a tumor-associated signature, on remains ambiguous. Here, we utilize wide range densities alter...
Diabetes mellitus is a complex metabolic disorder that associated with an increased risk of breast cancer. Despite this correlation, the interplay between tumor progression and diabetes, particularly regard to stiffening extracellular matrix, still mechanistically unclear. Here, we established murine model where hyperglycemia was induced before development. Using model, in vitro systems, patient samples, show increases growth, matrix stiffness, glycation, epithelial-mesenchymal transition...
Emergency medical service provides a variety of services for those in need emergency care. One the major challenges encountered by providers is selecting appropriate locations ambulance stations. Prior works measure spatial proximity under Euclidean space or static road network. In this paper, we focus on locating stations using real traffic information so as to minimize average travel-time reach requests. To end, estimate segments GPS trajectories and propose an efficient PAM-based...
Intratumor heterogeneity is a well-established hallmark of cancer that impedes research, diagnosis, and treatment. Previously, we phenotypically sorted human breast cells based on migratory potential. When injected into mice, highly were weakly metastatic metastatic. The purpose this study was to determine whether these interact with each other in vitro or vivo.To assess the relationship between cell migration fitness, MDA-MB-231 SUM159PT triple negative subpopulations assayed separately 1:1...
Cell migration during many fundamental biological processes including metastasis requires cells to traverse tissue with heterogeneous mechanical cues that direct as well determine force and energy requirements for motility. However, the influence of discrete structural on remains challenging they are often coupled. Here, we decouple pro-invasive collagen fiber alignment tension study their individual impact migration. When presented both cues, preferentially travel in axis against alignment....
Cancer cell migration is highly heterogeneous, and the migratory capability of cancer cells thought to be an indicator metastatic potential. It becoming clear that a does not have inherently metastasize, with weakly often found metastatic. However, mechanism through which escape from primary tumor remains unclear. Here, utilizing phenotypically sorted human breast cells, we demonstrate disseminate via communication stromal cells. While are capable single migration, rely on cell-cell...
Cell contractility regulates multiple cell behaviors which contribute to both normal and pathological processes. However, measuring remains a technical challenge in complex biological samples. The current state of the art technologies employed measure have inherent limitations that greatly limit experimental conditions under they can be used. Here, we use quantitative polarization microscopy extract information about contractility. We show optical retardance signal measured from body is...
Abstract Docetaxel and cabazitaxel are guideline-chemotherapy treatments for metastatic castration-resistant prostate cancer (mCRPC), which comprises the majority of deaths. TNF-related apoptosis inducing ligand (TRAIL) is an anticancer agent that selectively cytotoxic to cells; however, many human cancers resistant TRAIL. In this study, we sensitized androgen-independent TRAIL-resistant cells TRAIL-mediated via taxane therapy examined mechanism sensitization. DU145 PC3 displayed no...
Diabetes is associated with increased risk of breast cancer and worse prognoses for patients. Hyperglycemia can result in glycation, the process wherein crosslinkages are formed between sugars extracellular matrix (ECM) proteins through formation advanced glycation endproducts (AGEs). Although accumulation AGEs occurs naturally vivo over time, it greatly accelerated by hyperglycemic environment diabetic AGE has been linked to stiffening-related diseases such as hypertension, metastasis,...
In large-scale public security equipment projects, long-term operation often leads to failures and other problems, so it is particularly important choose the appropriate maintenance (O&M) scheme based on content of work orders. However, there are a variety models in orders; not only data complex, but also due long project cycle, problems such as loss content, which bring great challenges O&M work. This paper defines these “3-No problems”: inconsistency, inaccuracy, incompleteness....
Peach gum polysaccharides (PGPs) exhibit antioxidant and antibacterial activities. Nevertheless, the effect of PGPs on spermatogenesis KKAy mice with impaired reproduction system remains undetermined.PGPs were extracted hot water. randomly divided into two groups, namely, control (treated 100 mg/kg PGPs).Oral administration decreased levels serum triglyceride, total cholesterol, low-density lipoprotein fasting blood glucose, plasma insulin, nitrate nitrogen level in testes mice. Moreover,...
ABSTRACT Abnormal tensional cellular homeostasis is now considered a hallmark of cancer. Despite this, the origin this abnormality remains unclear. In work, we investigated role tissue transglutaminase 2 (TG2, also known as TGM2), protein associated with poor prognosis and increased metastatic potential, its relationship to EGF receptor in regulation mechanical state tumor cells. Remarkably, observed TG2-mediated modulation focal adhesion composition well stiffness-induced FAK activation,...