A5029224233- interferon and immune responses
- Viral Infections and Vectors
- Respiratory viral infections research
- Mosquito-borne diseases and control
- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- Burkholderia infections and melioidosis
- Viral Infections and Outbreaks Research
- Virus-based gene therapy research
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Tracheal and airway disorders
- Adipose Tissue and Metabolism
- Ubiquitin and proteasome pathways
- HIV-related health complications and treatments
- Influenza Virus Research Studies
- Virology and Viral Diseases
- Peptidase Inhibition and Analysis
- Viral Infections and Immunology Research
- Pneumonia and Respiratory Infections
- Parvovirus B19 Infection Studies
- RNA modifications and cancer
Rutgers, The State University of New Jersey
2022-2024
The University of Texas Medical Branch at Galveston
2015-2023
Rütgers (Germany)
2022
Rutgers Sexual and Reproductive Health and Rights
2022
Texas Tech University Health Sciences Center
2014-2018
Texas Tech University
2014-2018
Sanford Burnham Prebys Medical Discovery Institute
2011
All India Institute of Medical Sciences
2006-2010
Abstract Background Co-circulation of multiple dengue virus serotypes has been reported from many parts the world including India, however concurrent infection with more than one serotype viruses in same individual is rarely documented. An outbreak hemorrhagic fever/dengue shock syndrome (DHF/DSS) occurred and around Delhi 2006. This first report India high percentage infections different circulating during outbreak. Results Acute phase sera patients were tested for presence RNA by RT-PCR...
Abstract Aedes aegypti mosquitoes are common vectors for dengue virus and chikungunya virus. In areas where both viruses cocirculate, they can be transmitted together. During a outbreak in Delhi 2006, 17 of 69 serum samples were positive by reverse transcription–PCR; 6 viruses.
Abstract Background Acute lower respiratory tract infections (ALRI) are the major cause of morbidity and mortality in young children worldwide. Information on viral etiology ALRI from India is limited. The aim present study was to develop a simple, sensitive, specific cost effective multiplex PCR (mPCR) assay without post hybridization or nested steps for detection syncytial virus (RSV), influenza viruses, parainfluenza viruses (PIV1–3) human metapneumovirus (hMPV). Nasopharyngeal aspirates...
For efficient replication, viruses have developed mechanisms to evade innate immune responses, including the antiviral type-I interferon (IFN-I) system. Nipah virus (NiV), a highly pathogenic member of Paramyxoviridae family (genus Henipavirus), is known encode for four P gene-derived viral proteins (P/C/W/V) with IFN-I antagonist functions. Here we report that NiV matrix protein (NiV-M), which important assembly and budding, can also inhibit responses. production requires activation...
Acute respiratory infection (ARI) is a major killer of children in developing countries. Although the frequency ARI similar both developed and countries, mortality due to 10-50 times higher Viruses are common causes among such children, yet disease burden these infections rural communities unknown.A prospective longitudinal study was carried out enrolled from two Indian villages at birth followed weekly for development ARI, classified as upper infection, acute lower (ALRI), or severe ALRI....
Ebola virus (EBOV), a member of the Filoviridae family, is highly pathogenic that causes severe hemorrhagic fever in humans and responsible for epidemics throughout sub-Saharan, central, West Africa. The EBOV genome encodes VP35, an important viral protein involved replication by acting as essential cofactor polymerase well potent antagonist host antiviral type I interferon (IFN-I) system. By using mass spectrometry analysis coimmunoprecipitation assays, we show here VP35 ubiquitinated on...
Chronic immune activation that persists despite anti-retroviral therapy (ART) is the strongest predictor of disease progression in HIV infection. Monocyte/macrophages HIV-infected individuals are known to spontaneously secrete cytokines, although neither mechanism nor molecules involved known. Here we show overexpression newly described co-stimulatory molecule, PD1 homologue (PD-1H) human monocyte/macrophages sufficient induce spontaneous secretion multiple cytokines. The process requires...
Type I interferons (IFN-I) are essential to establish antiviral innate immunity. Unanchored (or free) polyubiquitin (poly-Ub) has been shown regulate IFN-I responses. However, few unanchored poly-Ub interactors known. To identify factors regulated by in a physiological setting, we developed an approach isolate from lung tissue. We identified the RNA helicase DHX16 as potential pattern recognition receptor (PRR). Silencing of cells and vivo diminished responses against influenza virus. These...
Galla rhois is a commonly used traditional medicine for the treatment of pathogenic bacteria in Korea as well other parts Asia. Methyl gallate (MG), major component Rhois, exhibits strong antibacterial activity, but its mechanism action against Salmonella spp. unclear. In present study, we investigated actions MG Salmonella. The activity determined by broth dilution method indicated that strains ranged from 3.9 to 125 µg/ml. vitro bacterial viability test significantly decreased over 40%...
Burkholderia pseudomallei is a Gram-negative facultative intracellular bacterium and the causative agent of melioidosis, severe infectious disease found throughout tropics. This organism closely related to mallei, etiological glanders which primarily affects equines. These two pathogenic bacteria are classified as Tier 1 select agents due their amenability aerosolization, limited treatment options, lack an effective vaccine. We have previously successfully demonstrated immunogenicity...
CCR5 disruption by zinc finger nucleases (ZFNs) is a promising method for HIV-1 gene therapy. However, successful clinical translation of this strategy necessitates the development safe and effective delivery into relevant cells. We used non-integrating lentivirus (NILV) transient expression ZFNs pseudotyped virus with HIV-envelope targeted to CD4(+) T Both activated resting primary cells transduced CCR5-ZFNs NILV showed resistance infection in vitro. Furthermore, from seronegative...
Multiplexed miRNA-based shRNAs (shRNA-miRs) could have wide potential to simultaneously suppress multiple genes. Here, we describe a simple strategy express large number of shRNA-miRs using minimal flanking sequences from endogenous miRNAs. We found that sequence 30 nucleotides the miRNA duplex was sufficient for efficient processing shRNA-miRs. inserted in tandem, each containing different miRNA. Deep sequencing transfected cells showed accurate individual and their expression did not...
WNV is a mosquito-borne flavivirus that poses threat to human health across large discontinuous areas throughout the world. Infection with results in febrile illness, which can progress severe neurological disease. Currently, there are no approved treatment options control infection. Understanding cellular immune responses regulate viral replication important diversifying resources available WNV. Here, we show elimination of TRIM6 cells an increase and alters expression function other...
Ebola virus (EBOV) VP35 is a polyfunctional protein involved in viral genome packaging, polymerase function, and host immune antagonism. The mechanisms regulating VP35’s engagement different functions are not well-understood. We previously showed that the E3 ubiquitin ligase TRIM6 ubiquitinates at lysine 309 (K309) to facilitate replication. However, how K309 ubiquitination regulates function of as co-factor precise stage(s) EBOV replication cycle require known. Here, we generated...
Abstract Human bocavirus (HBoV) is a new human parvovirus identified in children with respiratory tract disease. Nasopharyngeal aspirates were collected from 305 <5 years of age acute infection April 2005 to March 2007 and screened for the presence HBoV by two separate sets polymerase chain reaction (PCR) described previously. Twenty‐two (7.2%) who had found be positive both PCR. The main clinical symptoms cough (95%), runny nose (64%), fever (59%). In samples, was together syncytial...
Background Glanders caused by Burkholderia mallei is a re-emerging zoonotic disease affecting solipeds and humans. Furthermore, B. genetically related to pseudomallei, which the causative agent of melioidosis. Both facultative intracellular bacteria are classified as tier 1 select biothreat agents. Our previous study with ΔtonB Δhcp1 (CLH001) live-attenuated vaccine demonstrated that it attenuated, safe protective against wild-type strains in susceptible BALB/c mouse model....
Abstract Melioidosis, caused by Burkholderia pseudomallei ( Bpm ), lacks a vaccine. We identify the immune correlates of protection induced B. mallei Δ tonB hcp1 (CLH001) and PBK001) vaccines against inhalational melioidosis. Mucosal immunization with either vaccine generates -specific IgM IgG (IgG 2 b/c > 1 3 ) antibodies in sera lungs, lung IgA antibodies. Sera confers complement-independent bactericidal activity macrophages opsonophagocytic uptake but is insufficient passive transfer...
Respiratory tract vaccination has an advantage of needle-free delivery and induction mucosal immune response in the portal SARS-CoV-2 entry. We utilized human parainfluenza virus type 3 vector to generate constructs expressing full spike (S) protein SARS-CoV-2, its S1 subunit, or receptor-binding domain, tested them hamsters as single-dose intranasal vaccines. The construct bearing full-length S induced high titers neutralizing antibodies specific domains critical functions. Robust memory T...