A dysbiotic microbiome can potentially contribute to the pathogenesis of many different diseases including cancer. Breast cancer is second leading cause death in women. Thus, we investigated diversity four major types breast cancer: endocrine receptor (ER) positive, triple Her2 positive and negative cancers. Using a whole genome transcriptome amplification pan-pathogen microarray (PathoChip) strategy, detected unique common viral, bacterial, fungal parasitic signatures for each types. These...

// Sagarika Banerjee 1 , Tian 2 Zhi Wei Natalie Shih 3 Michael D. Feldman James C. Alwine 4 George Coukos 5 Erle S. Robertson Department of Otorhinolaryngology-Head and Neck Surgery, University Pennsylvania, Philadelphia, United States America Computer Science, New Jersey Institute Technology, Newark, Jersey, Pathology Laboratory Medicine, Cancer Biology, Oncology, Hospital Lausanne (CHUV), Lausanne, Switzerland Correspondence to: Robertson, email: erle@mail.med.upenn.edu Keywords:...

Abstract Infectious agents are the third highest human cancer risk factor and may have a greater role in origin and/or progression of cancers related pathogenesis. Thus, knowing specific viruses microbial associated with type provide insights into cause, diagnosis treatment. We utilized pan-pathogen array technology to identify signatures triple negative breast (TNBC). This detects low copy number fragmented genomes extracted from formalin-fixed paraffin embedded archival tissues. The...

Abstract We have established a microbiome signature for prostate cancer using an array-based metagenomic and capture-sequencing approach. A diverse (viral, bacterial, fungal parasitic) was observed in the samples compared with benign hyperplasia controls. Hierarchical clustering analysis identified three distinct cancer-specific signatures. The signatures correlated different grades, stages scores of cancer. Thus, potentially provides clinical diagnosis outcome predictions. array data were...

Abstract Alterations to the natural microbiome are linked different diseases, and presence or absence of specific microbes is directly related disease outcomes. We performed a comprehensive analysis with unique cohorts four subtypes breast cancer (BC) characterized by their microbial signatures, using pan-pathogen microarray strategy. The signature (includes viruses, bacteria, fungi, parasites) each tumor subtype was correlated clinical data identify prognostic potential. BC had viromes...

The microbiome is fundamentally one of the most unique organs in human body. Dysbiosis can result critical inflammatory responses and pathogenesis contributing to neoplastic events. We used a pan-pathogen array technology (PathoChip) coupled with next-generation sequencing establish microbial signatures oral oropharyngeal squamous cell carcinomas (OCSCC/OPSCC). Signatures for DNA RNA viruses including oncogenic viruses, gram positive negative bacteria, fungi parasites were detected. Cluster...

The integrated framework of in silico , vitro and vivo approaches identifies bacterial strains with the potential to degrade prebiotics, produce neuroactive molecules, alter stress-related gene expression behaviour a zebrafish model.

ABSTRACT Epstein-Barr virus (EBV) employs various strategies for long-term survival, including the expression of non-coding RNAs (ncRNAs). This study uncovers and characterizes two novel EBV-encoded ncRNAs, p7 p8, which are upregulated during lytic reactivation interact with both viral host genomes. These ncRNAs bind to cellular RNA transcripts, significantly reducing ARMCX3 mRNA levels, while p8 also influences PTPN6 RPL24 expressions. Although does not directly LMP1 but found downregulate...

Abstract Human metapneumovirus (hMPV) causes acute respiratory infections in children and adults. It is classified into two major genetic lineages each lineage sublineages. The purpose of the study was to identify characterize hMPV who presented All India Institute Medical Sciences, New Delhi, with infection from April 2005 March 2007. By reverse‐transcription polymerase chain reaction, detected 21 (3%) 662 nasopharyngeal samples none 120 control children. Seven (33%) infected required...

Abstract The effects of two doses (50 and 100 mg/kg body wt/day for 14 days) an 80% hydroalcohol extract Andrographis paniculata butylated hydroxyanisole (BHA) were examined on drug metabolizing enzymes, antioxidant glutathione content, lactate dehydrogenase (LDH) lipid peroxidation in the liver Swiss albino mice (6–8 weeks old). effect BHA also lung, kidney forestomach activities S‐transferase (GST), DT‐diaphorase (DTD), superoxide dismutase (SOD) catalase. A significant increase levels...

Abstract The effect of hydroalcoholic (80% ethanol, 20% water) extract leaves Aegle marmelos was examined on carcinogen-metabolizing phase-I and phase-II enzymes, antioxidant glutathione content, lactate dehydrogenase lipid peroxidation, using two doses dried (50 100 mg kg−1 daily for 14 days), in the liver mice. modulatory also extrahepatic organs (lung, kidney fore-stomach) effects activity S-transferase, DT-diaphorase, superoxide dismutase catalase. Extract treatment significantly...

The cellular adhesion molecule LFA-1 and its ICAM-1 ligand play an important role in promoting HIV-1 infectivity transmission. These molecules are present on the envelope of virions integral components HIV virological synapse. However, activation is required to convert active conformation that has high affinity binding for ICAM-1. This study evaluates whether such can be induced by itself. data show gp120 was sufficient trigger fully quiescent naïve CD4 T cells a CD4-dependent manner, these...

The present study reports the modulatory influence of alcoholic extract from leaves Ocimum sanctum on activities cytochrome p-450, b5, and aryl hydrocarbon hydroxylase enzymes in liver glutathione-S-transferase reduced glutathione level liver, lung, stomach mouse. Oral treatment with leaf at 400 800 mg/kg body wt for 15 days would significantly elevate p-450 (p < 0.05), b5 0.01, p 0.001), S-transferase 0.05, 0.01), all which are important detoxification carcinogens as well mutagens. Moreover...

We report the chemopreventive property of an ethanollc extract leaves Ocimum sanctum (a traditional medicinal plant) on 7,12-dlmethylbenz[a]anthracene Induced skin papillomagenesis In male Swiss albino mice. A significant reduction values tumor Incidence, average number tumors per bearing mice and cumulative paplllomas was observed treated topically with leaf O. at either perMnltlatlonal, post-lnltlatlonal stages or continuously perl- as compared to corresponding control group. Topical...

Abstract Background Of the many neurotransmitters in humans, gamma-aminobutyric acid (GABA) shows potential for improving several mental health indications such as stress and anxiety. The microbiota-gut-brain axis is an important pathway GABAergic effects, microbially-secreted GABA within gut can affect host outcomes. Understanding molecular characteristics of production by microbes offer insight to novel therapies health. Results Three strains Levilactobacillus brevis with syntenous...

The modulatory potential of Spirulina fusiformis was observed on the hepatic and extrahepatic carcinogen metabolizing enzymes in Swiss albino mice at a dose 800 mg/kg b.w. given orally. A significant reduction cytochrome P450 content group treated with comparison control group. glutathione S-transferase activity induced significantly by treatment. There no change after animals were fed Spirulina. Copyright © 1999 John Wiley & Sons, Ltd.