A5040666669- RNA Interference and Gene Delivery
- Dendrimers and Hyperbranched Polymers
- Advanced Polymer Synthesis and Characterization
- Rare-earth and actinide compounds
- Iron-based superconductors research
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Physics of Superconductivity and Magnetism
- Immunotherapy and Immune Responses
- Nanoplatforms for cancer theranostics
- Polymer Surface Interaction Studies
- Surfactants and Colloidal Systems
- Virus-based gene therapy research
- Gold and Silver Nanoparticles Synthesis and Applications
- DNA and Nucleic Acid Chemistry
- Lipid Membrane Structure and Behavior
- Organic and Molecular Conductors Research
- biodegradable polymer synthesis and properties
- Advanced Drug Delivery Systems
- Hydrogels: synthesis, properties, applications
- Chemical Synthesis and Analysis
- Advanced Condensed Matter Physics
- Bone health and osteoporosis research
- Protein Interaction Studies and Fluorescence Analysis
- Refrigeration and Air Conditioning Technologies
Osaka Metropolitan University
2022-2025
Osaka Prefecture University
2015-2024
Tokyo Metropolitan University
2024
Tottori University
2024
Sapporo Medical University Hospital
2024
Suita Tokushukai Hospital
2024
National Institute of Technology, Kagoshima College
2024
Kyushu University
1988-2023
Jikei University School of Medicine
2022
Graduate School USA
2014-2020
Drug smuggling: Micelles made up of block copolymers and the anticancer drug adriamycin (ADR), which is linked to polymer by a pH-sensitive linker, have been prepared (see picture). Treatment cancer cells with micelles shows that are transported into cell endocytosis, thus bypassing cell-membrane transporters. In cell, decrease in pH value triggers release ADR, retains its cytotoxic effect. The released ADR fluoresces allows localization within be detected. Supporting information for this...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFormation of Polyion Complex Micelles in an Aqueous Milieu from a Pair Oppositely-Charged Block Copolymers with Poly(ethylene glycol) SegmentsAtsushi Harada and Kazunori KataokaCite this: Macromolecules 1995, 28, 15, 5294–5299Publication Date (Print):July 1, 1995Publication History Published online1 May 2002Published inissue 1 July 1995https://pubs.acs.org/doi/10.1021/ma00119a019https://doi.org/10.1021/ma00119a019research-articleACS...
Molecular recognition based on length was found to occur between oppositely charged pairs of flexible and randomly coiled block copolymers in an aqueous milieu. Matched with the same lengths polyanions polycations exclusively formed even mixtures different lengths. These assemblies segments matched chain then larger core-shell–type supramolecular extremely narrow size distribution due strict phase separation core- shell-forming segments.
We have examined the cytoskeletal architecture and its relationship with synaptic vesicles in synapses by quick-freeze deep-etch electron microscopy (QF.DE). The main elements presynaptic terminals (neuromuscular junction, electric organ, cerebellar cortex) were actin filaments microtubules. formed a network frequently associated closely plasma membranes active zones. Short, linking strands approximately 30 nm long found between vesicles, microtubules vesicles. Fine (30-60 nm) also...
A core−shell-type supramolecular assembly, a polyion complex micelle, was prepared in this study from chicken egg white lysozyme and poly(ethylene glycol)−poly(aspartic acid) block copolymer (PEG-P(Asp)) through electrostatic interaction aqueous medium. Lysozyme/PEG-P(Asp) micelles thus had an extremely narrow distribution (μ2/Γ2 < 0.04) with average diameter of 47 nm dynamic light scattering measurements. No precipitate formation observed even after 1 month standing at ambient temperature,...
A block catiomer polyplex, showing a high stability in the extracellular medium and an efficient release of plasmid DNA (pDNA) intracellular compartment, was developed by controlling both cationic charge disulfide cross-linking densities backbone polycations. Poly(ethylene glycol)-poly(l-lysine) copolymer (PEG-PLL) thiolated using either two thiolation reagents, N-succinimidyl 3-(2-pyridyldithio)propionate (SPDP) or 2-iminothiolane (Traut's reagent), to investigate effects on properties...
Cytoplasmic dynein, a minus end–directed, microtubule-based motor protein, is thought to drive the movement of membranous organelles and chromosomes. It massive complex that consists multiple polypeptides. Among these polypeptides, cytoplasmic dynein heavy chain (cDHC) constitutes major part this complex. To elucidate function we have produced mice lacking cDHC by gene targeting. cDHC−/− embryos were indistinguishable from cDHC+/−or cDHC+/+ littermates at blastocyst stage. However, no found...
This study is devoted to the development of novel glucose-responsive polymers that operate under physiological conditions (pH 7.4, 37 °C), aiming for future use in a self-regulated insulin delivery system treat diabetes mellitus. The approach involves newly synthesized phenylborate derivative {4-(1,6-dioxo-2,5-diaza-7-oxamyl) phenylboronic acid, DDOPBA} possessing an appreciably low pKa (∼7.8) as glucose-sensing moiety, well adoption poly(N-isopropylmethacrylamide), PNIPMAAm, main chain...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTEnvironment-Sensitive Stabilization of Core−Shell Structured Polyion Complex Micelle by Reversible Cross-Linking the Core through Disulfide BondY. Kakizawa, A. Harada, and K. KataokaView Author Information Department Materials Science Graduate School Engineering, The University Tokyo 7-3-1 Hongo, Bunkyo-ku, 113-8656, Japan Cite this: J. Am. Chem. Soc. 1999, 121, 48, 11247–11248Publication Date (Web):November 19, 1999Publication History...
Glutathione (GSH)-sensitive stabilization of polyion complex (PIC) micelles entrapping antisense oligonucleotide (ODN) was achieved by the reversible cross-linking core through disulfide bonds, aiming at development a novel DNA carrier system for therapy following systemic administration. Thiolated poly(ethylene glycol)-block-poly(l-lysine) (PEG−thioPLL) and ODN spontaneously associated to form PIC with cross-linked bonds. The diameters were similar those non-cross-linked determined be about...
"Drogenschmuggel": Präpariert wurden Micellen aus Blockcopolymeren und dem Tumortherapeutikum Adriamycin (ADR), das über einen pH-sensitiven Linker an Polymer gebunden ist (siehe Bild). Die gelangen durch Endocytose, unter Umgehung der Zellmembrantransporter, in die Krebszelle. Innerhalb Zelle löst eine Senkung des pH-Wertes Freisetzung von ADR aus, dessen Position fluoreszenzspektroskopisch bestimmt wird.
Abstract Background The investigation into the intracellular mechanisms for gene expression has acquired great impetus improvement of transfection efficiency by a non‐viral delivery system. Methods Intracellular trafficking as well release plasmid DNA (pDNA) complexed with polycations, including linear and branched polyethylenimine (LPEI, BPEI) poly(L‐lysine) (PLL), were explored under confocal microscopy using fluorescence resonance energy transfer (FRET) between pair donor–acceptor...
Photodynamic therapy (PDT) for exudative age-related macular degeneration (AMD) was evaluated using a supramolecular nanomedical device, that is, novel dendritic photosensitizer (DP) encapsulated by polymeric micelle formulation. The characteristic structure of the DP prevents aggregation its core sensitizer, thereby inducing highly effective photochemical reaction. With selective accumulation on choroidal neovascularization (CNV) lesions, this treatment resulted in remarkably efficacious...