A5075323100- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Electrospun Nanofibers in Biomedical Applications
- Hydrogels: synthesis, properties, applications
- Tissue Engineering and Regenerative Medicine
- biodegradable polymer synthesis and properties
- Nanoparticle-Based Drug Delivery
- 3D Printing in Biomedical Research
- Advanced Drug Delivery Systems
- Mesenchymal stem cell research
- Supramolecular Self-Assembly in Materials
- Advanced biosensing and bioanalysis techniques
- Mesoporous Materials and Catalysis
- Monoclonal and Polyclonal Antibodies Research
- Surgical Sutures and Adhesives
- Diatoms and Algae Research
- Polymer crystallization and properties
- Carbon dioxide utilization in catalysis
- Enzyme Catalysis and Immobilization
- Advanced Polymer Synthesis and Characterization
- Polymer Surface Interaction Studies
- Lipid Membrane Structure and Behavior
- Synthesis and properties of polymers
- Veterinary Pharmacology and Anesthesia
- Liver physiology and pathology
Kansai University
2017-2024
Tohoku University
2021-2024
Osaka Prefecture University
2014-2021
Graduate School USA
2021
Okayama University
2015
Hyaluronic acid (HA)-coated biodegradable polymeric micelles were developed as nanoparticulate vaccine delivery systems to establish an effective nasal vaccine. We previously reported HA-coated prepared by forming a polyion complex (PIC) of poly(L-lysine)-b-polylactide (PLys+-b-PLA) and HA. The exhibited specific accumulation in HA receptor-expressing cells extremely high colloidal stability under diluted blood conditions. In this study, model antigen, ovalbumin (OVA), adjuvant...
To establish peptide vaccine-based cancer immunotherapy, we investigated the improvement of antigenic peptides by encapsulation with pH-sensitive fusogenic polymer-modified liposomes for induction antigen-specific immunity. The were prepared modification egg yolk phosphatidylcholine and l-dioleoyl phosphatidylethanolamine 3-methyl-glutarylated hyperbranched poly(glycidol) (MGlu-HPG) loaded derived from ovalbumin (OVA) OVA-I (SIINFEKL), OVA-II (PSISQAVHAAHAEINEAPβA), which bind, respectively,...
Developing delivery vehicles that achieve drug accumulation in the liver and transferability into hepatic stellate cells (HSCs) across sinusoidal endothelium is essential to establish a treatment for fibrosis. We previously developed hyaluronic acid (HA)-coated polymeric micelles exhibited affinity endothelial cells. HA-coated possess core–shell structure of self-assembled biodegradable poly(l-lysine)-b-poly(lactic acid) AB-diblock copolymer (PLys+-b-PLLA), its exterior coated with HA...
Label-free measurement is essential to understand the metabolism of drug molecules introduced into cells. Raman imaging a powerful method investigate intracellular because it provides in situ label-free observation molecules. In this study, we propose that can be used not only observe distribution but also quantitatively visualize concentration reflecting each organelle single living cell using band extracellular water as an intensity standard. We dissolved poorly water-soluble...
Adipose-derived stem cell (AdSC) has been attracting attention as a convenient source. Not only AdSC can differentiate into various tissue cells, but it also accelerate proliferation, anti-inflammation, and angiogenesis by secreting paracrine factors. Studies have demonstrated treatment of ischemic heart. However, an improvement in the remaining live AdSCs administered at injected site while maintaining factor secretion is desired to achieve effective regenerative medicine. We previously...
The zwitterionic phospholipid polymer poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate) (PMB) is amphiphilic copolymer, and it has been reported to directly penetrate cell membranes have good cytocompatibility. Conventional PMBs are linear-type random copolymers that polymerized by a free radical polymerization technique. In contrast, star-shaped polymers, or simple branched-type unique properties compared the linear types, for example, viscosity based on effect of...
Abstract Topological gels possess structures that are cross‐linked only via physical constraints; ideally, no attractive intermolecular interactions act between their components, which yields interesting properties. However, most reported previous topological were synthesized based on supramolecular interlocked such as polyrotaxane, for essential. Here, we synthesize a water‐soluble “molecular net” (MN) with large molecular weight and three‐dimensional network structure using poly(ethylene...
Postoperative adhesion remains a problem in surgery and causes postoperative complications. Laparoscopic is now common, making it increasingly important to develop injectable formulations of barriers that can be applied during such surgeries. Temperature-responsive polymer (IP) systems exhibiting sol-to-gel transition response temperature are promising candidates as effective conveniently laparoscopic surgery. We previously developed IP temperature-responsive irreversible gelation based on...
Topological gels possess structures that are cross-linked only via physical constraints; ideally, no attractive intermolecular interactions act between their components, which yields interesting properties. However, most reported previous topological were synthesized based on supramolecular interlocked such as polyrotaxane, for essential. Here, we synthesize a water-soluble "molecular net" (MN) with large molecular weight and three-dimensional network structure using poly(ethylene glycol)....
Abstract Here, a vaccine delivery system is developed that attains sustained release of antigens (ovalbumin; model antigen) and adjuvants (CpG‐DNA; oligodeoxynucleotides containing unmethylated cytosine‐guanine motifs) using biodegradable temperature‐responsive injectable polymers (IP). Previously IP was prepared exhibited sol‐to‐gel transition subsequent chemical cross‐linking. The used triblock copolymers, poly(ε‐caprolactone‐ co ‐glycolide)‐ b ‐poly(ethylene glycol)‐ ‐poly(ε‐caprolactone‐...
Transdermal drug delivery systems are a key technology for skin-related diseases and cosmetics development. The of active ingredients to an appropriate site or target cells can greatly improve the efficacy medical cosmetic agents. For this study, liposome-based transdermal were developed using pH-responsive phytosterol derivatives as liposome components. Succinylated (Suc-PS) 2-carboxy-cyclohexane-1-carboxylated (CHex-PS) synthesized by esterification hydroxy groups phytosterol. Modification...
We investigated the release behavior of glucagon-like peptide-1 (GLP-1) from a biodegradable injectable polymer (IP) hydrogel. This hydrogel shows temperature-responsive irreversible gelation due to covalent bond formation through thiol-ene reaction. In vitro sustained GLP-1 an IP formulation (F(P1/D+PA40)) was observed compared with reversible (physical gelation) (F(P1)). Moreover, pharmaceutically active levels were maintained in blood after subcutaneous injection into rats. system should...
Carboxylated polyamidoamine dendron lipid-containing liposomes induced precisely controlled content release within intracellular organelles, which antigen-specific cellular and humoral immune responses, leading to antitumor effects.
A reversible and cytocompatible cell immobilization polymer matrix with a rapid dissociation rate was prepared using zwitterionic phospholipid bearing phenylboronic acid poly(vinyl alcohol) (PVA). spontaneously forming hydrogel is reported for use as which caused no invasive damage to the cells. To improve possibility of applying hydrogels matrix, stimuli-responsive designed have more ease recovery immobilized In this study, containing 3-methacrylamide (MAPBA) unit synthesized. The...
Small-caliber artificial blood vessels with inner diameters of smaller than 4 mm have not been put into practical use because early thrombus formation and graft occlusion. To realize small-caliber anti-thrombus property long-term patency, one the promising approaches is endothelialization lumen by tissue engineering approaches. Integrin α4β1 on endothelial cell membrane known to act as a receptor for Arg-Glu-Asp-Val (REDV) tetra-peptide, this peptide can be used specific ligand introduce...
Abstract Adipose-derived stem cells (AdSCs), a type of mesenchymal cell, are expected to be applicable regenerative medicine and cellular delivery systems. The maintenance cell multipotency control the differentiation direction important for these applications. However, is widely believed depend on physical properties their scaffold. In this study, we explored whether AdSCs, that is, ability differentiate into multiple cells, maintained when they removed from injectable polymer (IP)...
Stereocomplex (SC) crystallization, homocrystallization, and polymorphism of poly(l-lactic acid-co-l-alanine) [P(LLA-LAL)] poly(d-lactic acid-co-d-alanine) [P(DLA-DAL)] copolymers with wide alanine unit content ranges from 0 to 21 22 mol% are investigated for melt-crystallization. P(LLA-LAL)/P(DLA-DAL) blends crystallize compared unblended P(LLA-LAL) P(DLA-DAL) samples, due facile SC crystallization homocrystallization. The phase diagrams the samples [α- δ-form homocrystallites] blend (SC...