Systemic administration of IL-12 greatly reduced the hepatic metastases i.v.-injected liver metastatic EL4 tumor cells in C57BL/6 +/+ and nu/nu mice. Cytotoxic assay vitro revealed that enhanced cytotoxicity mononuclear (MNC) against various NK- sensitive -resistant targets, including cells, whereas only slight or moderate augmentation was observed splenocytes normal nude After administration, MNC increased number showed vigorous proliferation vitro. Hepatic control mice contain alpha beta T...

Transplantation of bone marrow cells from nonobese diabetic (NOD) mice, a model for type 1 diabetes mellitus, to C3H/HeN which express I-E alpha molecules and have aspartic acid at residue 57 the I-A beta chain, induced insulitis followed by overt in recipient mice more than 40 weeks after transplantation. When cyclosporin A, perturbs T-cell functions, was injected intraperitoneally into [NOD----C3H/HeN] chimeric daily month, developed within 20 following (NZW x BXSB)F1 develop lupus...

MRL/MP-Ipr/Ipr (MRL/Ipr) mice possess radioresistant (9.5 Gy) abnormal stem cells and show a recurrence of autoimmune diseases within 5 mo conventional allogeneic bone marrow transplantation. We recently have found that the MHC preference exists between hemopoietic stromal cells; when bones are engrafted, donor-derived present in engrafted can migrate into recipient marrows, which replaced with both hematopoietic cells. Based on these findings, we attempted to prevent MRL/Ipr by...

We demonstrate herein evidence that IL-12-activated alpha beta T cells with intermediate TCR (NK1+ TCRint cells) in the liver inhibit metastases lung as well of i-v. injected tumors. IL-12 administration enhanced NK1 expression NK1+ (NK1high) and increased CD4 weakly positive (CD4low) cells, while both CD4+ double-negative were proportionally diminished. Accordingly, major parts NK1high are CD4low most these V 8+ cells. The cytotoxic assays IL-12-stimulated hepatic mononuclear after...

We examined the effects of transparent fibroin film (silk film) on full-thickness skin wounds. Full-thickness dermatotomies (15 mm x 9 mm) were prepared dorsal wall CRJ:CD-1 nu/nu (ICR nu/nu) mice. The area wounds dressed with silk was reduced to 10% that made by dermatotomy 14 days after and covered regenerated epidermis 21 dermatotomy. In contrast, less recovery epidermal regeneration found in a conventional hydrocolloid dressing (Duro Active). Furthermore, only partial incomplete epidemal...

Long-term effects of allogeneic bone marrow transplantation (ABMT) across major histocompatibility complex barriers were studied in (NZB x NZW)F1 (B/W), BXSB, and MRL/Mr-lpr-lpr (MRL/lpr) mice with established autoimmune disease at the time ABMT. In BXSB or B/W mice, ABMT cured all aspects disease. Glomerular damage, revealed by histological study was dramatically improved. Serological abnormalities immunologic functions also normalized. Correction advanced renal regularly lasted greater...

In addition to T cell differentiation in the thymus, we have recently reported that extrathymic occurs preferentially sinusoids of liver. Although this pathway is relatively minor normal mice, it becomes predominant mice with autoimmune diseases, athymic and aged mice. present study, injection male C3H/He 6-8 wk age, 1 mg estrogen resulted an increase mononuclear cells (MNC) yielded from liver a drastic decrease thymocytes approximately 10 d after such injection. This unique modulation was...

The liver is a major site of generation extrathymic T cells with unique properties (e.g., expressing intermediate TCR and containing self-reactive clones). We investigated herein whether the levels alpha beta varied in various organs as function age. A systematic examination number mononuclear BALB/c mice revealed that hepatic MNC increased age whereas thymocytes decreased. These changes were more striking fed under conventional conditions than specific pathogen-free condition. age-dependent...

Autoimmune MRL-lpr/lpr (lpr) mice develop severe lymphadenopathy, characterized by the accumulation of alpha beta-T cells with CD4-8- double negative (DN) phenotype, at onset disease. We previously demonstrated that liver is a major site for proliferation such DN cells. Herein, we further demonstrate large proportion in and other organs, except thymus, lpr have unique properties, as relatively dull TCR intensity, preponderance V beta 8+ cells, Pgp-1 expression. Interestingly, normal were...

Embryonic stem (ES) cells are pluripotent with the capacity to generate any type of cell. Here we describe isolation ES-like from canine blastocysts. Canine embryos were collected beagle bitches at day 11-16 first estrus. A total 80 normal obtained 15 dogs. Of embryos, 13 morulae stage, 39 blastocyst and 28 hatched stage. The inside or inner cell masses (ICMs) blastocysts isolated mechanically, cultured onto mouse embryonic fibroblasts (MEF) as feeder layers. Primary colonies formed in 0%...

In the present study we have characterized natural suppressor (NS) cells, which nonspecifically suppress mitogen responses and mixed-lymphocyte reaction. The strongest NS activity was found in a fraction of relatively low-density cells (1.063 less than p 1.075) obtained by equilibrium density centrifugation. Further purification characterization these using fluorescence-activated cell sorter indicated that wheat germ agglutinin (WGA)-positive potent activity, whereas WGA-negative no...

The etiopathogenesis of focal and segmental glomerular sclerosis (FGS) remains unknown. Using a new animal model for FGS (FGS mouse), we demonstrate here that bone marrow transplantation from normal mice to with high grade proteinuria (+ + +) ameliorates FGS, the cells or purified hemopoietic stem (HSCs) induces in mice. These findings strongly suggest is cell disorder; abnormalities may be genetically programmed at level HSCs.

In this report we examine the fate of donor cells injected via different routes. When PKH-26-labeled C57BL/6 (B6) spleen were intravenously (i.v.) into BALB/c mice, rejected within 3 days. contrast, when same B6 portal venously (p.v.) injected, they trapped in recipient liver. allogeneic or syngeneic whole bone marrow (BMC) a hemopoietic stem cell (HSC)-enriched fraction either i.v. p.v. accumulated The liver found to be wheat germ agglutinin (WGA)-positive HSC. thymocytes rapidly rejected....

To evaluate effects of transplantation bone marrow stromal cells (BMSCs) into the CSF for treatment chronic spinal cord injury in dogs that had not responded by 1 month after decompressive surgery.23 dogs.Dogs with paraplegia and loss nociception pelvic limbs at least surgery were assigned to or control groups. Dogs group received BMSCs injected 3 months surgery. did receive additional treatments. Improvements gait, proprioceptive positioning, evaluated use Texas Spinal Cord Injury Scale ≥ 6...

Thrombocytopenia (TTP) is a blood disease common to canines and human beings. Currently, there no valid therapy for this except transfusion. In study, we report the generation of canine induced pluripotent stem cells (ciPSCs) from embryonic fibroblasts, novel protocol creating mature megakaryocytes (MKs) functional platelets ciPSCs. The ciPSCs were generated using lentiviral vectors, differentiated into MKs on OP9 stromal supplemented with growth factors. Our presented in tightly domed shape...

A strategy to achieve regular and long lasting organ tissue allografts without using immunosuppressants and/or irradiation has been established for mice. One hundred percent of skin can be induced survive >350 days after transplantation if spleen cells from the same donors are first injected into portal vein recipients. The mechanisms underlying this long-term tolerance induction described as follows: (i) donor T facilitate acceptance allogeneic engraftment, (ii) donor-specific anergy is in...

In the kidney, prostaglandin (PG) E2 is main PG, playing important roles in maintaining homeostasis or development of pathological settings. Roles PGE2 renal lesions remain to be clarified. The expression patterns synthesis enzymes such as cyclooxygenase (COX)-1, COX-2 and microsomal PGE synthase (mPGES)-1, receptors (EP2 EP4) were examined cisplatin-induced rat failure. immunoexpressions for COX-1, mPGES-1 EP4 receptor increased exclusively affected tubules, but those EP2 not detected;...

Objective To assess the feasibility and safety of transplantation autologous bone marrow stromal cell ( BMSC ) in dogs with acute spinal cord injury SCI ). Study Design An open‐label single‐arm trial. Animals Dogs (n = 7) severe from T 6 to L 5, caused by vertebral fracture luxation. Methods Decompressive stabilization surgery was performed on Autologous s were obtained each dog's femur, cultured, then injected into lesion stage. Adverse events motor sensory function observed for >1 year...

Abstract. We examined the effects of transparent fibroin film (silk film) on full‐thickness skin wounds. Full‐thickness dermatotomies (15 mm × 9 mm) were prepared dorsal wall CRJ:CD‐1 nu/nu (ICR ) mice. The area wounds dressed with silk was reduced to 10% that made by dermatotomy 14 days after and covered regenerated epidermis 21 dermatotomy. In contrast, less recovery epidermal regeneration found in a conventional hydrocolloid dressing (Duro Active). Furthermore, only partial incomplete...