DNA origami involves the folding of long single-stranded into designed structures with aid short staple strands; such may enable development useful nanomechanical devices. Here we develop versatile sensing systems for a variety chemical and biological targets at molecular resolution. We have functional devices that can be used as 'single-molecule beacons', function pinching Using 'DNA pliers' forceps', which consist two levers ~170 nm connected fulcrum, various single-molecule inorganic...

A box-shaped 3D-DNA origami has been successfully constructed by selective closing of a preformed open motif, and identified atomic force microscopy dynamic light scattering analysis.

A new punched DNA origami assembly with periodic nanometer-scale wells has been successfully designed and constructed. Through the attachment of two biotins at edges each well, just one streptavidin (SA) tetramer (d = 5 nm) was size-selectively captured in 6.8 x 12 2.0 nm well; this allowed formation a 28 nm-period SA nanoarray individual molecules. The position capture can be fully controlled by placement well. Moreover, construction 2D tetramers through selective positioning any desired...

In nature, swarming behavior has evolved repeatedly among motile organisms because it confers a variety of beneficial emergent properties. These include improved information gathering, protection from predators, and resource utilization. Some organisms, e.g., locusts, switch between solitary swarm in response to external stimuli. Aspects have been demonstrated for supramolecular systems composed biomolecular motors cytoskeletal filaments, where cross-linkers induce large scale organization....

Abstract DNA aptamers have potential for disease diagnosis and as therapeutics, particularly when interfaced with programmable molecular technology. Here we combined specific the malaria biomarker Plasmodium falciparum lactate dehydrogenase ( Pf LDH) a origami scaffold. Twelve that recognise LDH were integrated into rectangular atomic force microscopy demonstrated incorporated preserve their ability to specifically bind target protein. Captured retained enzymatic activity protein-aptamer...

A split aptamer for adenosine triphosphate (ATP) was embedded as a recognition unit into two levers of nanomechanical DNA origami construct by extension and modification selected staple strands. An additional optical module in the stem comprised different cyanine–styryl dyes that underwent an energy transfer from green (donor) to red (acceptor) emission if ATP molecules were bound target molecule thereby brought close proximity. As result, triggered shape transition yielded fluorescence...

Cooperation is a strategy that has been adopted by groups of organisms to execute complex tasks more efficiently than single entities. increases the robustness and flexibility working permits sharing workload among individuals. However, utilization this in artificial systems at molecular level, which could enable substantial advances microrobotics nanotechnology, remains highly challenging. Here, we demonstrate transportation through cooperative action large number machines, photoresponsive...

Abstract Research on so‐called “chemical artificial intelligence” (CAI) is an emerging field with the aim of constructing information‐processing systems learning capabilities based chemical methodologies. This can be regarded as attempt to reconstruct Cybernetics using molecular systems. Many reaction computational abilities are proposed, but most fixed functions that deliver output for a given input. On other hand, AI system capability; namely, should variable and gradually change upon...

DNA nanotubes are cylinder-like structures formed from double-helical molecules whose helix axes fused at least twice by crossovers. It is potentially useful to use such tubes as sheaths around rodlike species that arise in biological systems and nanotechnology. seems easiest obtain sheathing joining two or more components an object rather than attempting thread the through a cavity tube. We report examples of containing specific number helices assembled half-tube components. These six-helix...

CONSPECTUS: Eight years have passed since the striking debut of DNA origami technique ( Rothemund, P. W. K. Nature 2006 , 440 297 - 302 ), in which long single-stranded is folded into a designed nanostructure, either 2D or 3D, with aid many short staple strands. The number proposals for new design principles structures seems to already reached peak. It apparent that study now entering second phase creating practical applications. development functional nanomechanical molecular devices using...

Desired enzyme nanoarrays patterned on a DNA origami scaffold were selectively isolated by affinity tag purification from pool of differently nanoarrays, and their enzymatic activity was successfully confirmed. As few as 12 histidine residues enough to hold huge complex with multiple proteins, 260 nm in length 5.2 MDa molecular weight, an immobilized metal resin.

Single-molecule pH sensors have been developed by utilizing molecular imaging of pH-responsive shape transition nanomechanical DNA origami devices with atomic force microscopy (AFM). Short fragments that can form i-motifs were introduced to pliers-like (DNA Origami Pliers), which consist two levers 170-nm long and 20-nm wide connected at a Holliday-junction fulcrum. Pliers be observed as in three distinct forms; cross, antiparallel parallel forms, cross is the dominant species when no...

DNA has been well-known for its applications in programmable self-assembly of materials. Nonetheless, utility origami, which offers more opportunity to realize complicated operations, very limited. Here we report a biomolecular motor system, microtubule-kinesin mediated by origami nanostructures. We demonstrate that rodlike motif facilitates microtubules into asters. A smooth-muscle like molecular contraction system also realized using the self-assembled exhibited fast and dynamic presence...

Hyaluronic acid (HA)-coated biodegradable polymeric micelles were developed as nanoparticulate vaccine delivery systems to establish an effective nasal vaccine. We previously reported HA-coated prepared by forming a polyion complex (PIC) of poly(L-lysine)-b-polylactide (PLys+-b-PLA) and HA. The exhibited specific accumulation in HA receptor-expressing cells extremely high colloidal stability under diluted blood conditions. In this study, model antigen, ovalbumin (OVA), adjuvant...

Stepwise introduction of multiple streptavidin tetramers into a nanoarray formed in stick-like punched DNA origami as well selective removal predetermined tetramer from the array were successfully achieved by applying programmed strand displacement technique to scaffold.

Biodegradable injectable polymer (IP) systems exhibiting temperature-responsive sol-to-gel transitions between room temperature and body have the potential for use in biomedical applications. However, gelation of such IP is a reversible process through physical cross-linking, hydrogels thus formed are likely to revert sol state under highly wet conditions after injection. In this study, biodegradable system irreversible transition by covalent bond formation was developed simple mixing...

Developing delivery vehicles that achieve drug accumulation in the liver and transferability into hepatic stellate cells (HSCs) across sinusoidal endothelium is essential to establish a treatment for fibrosis. We previously developed hyaluronic acid (HA)-coated polymeric micelles exhibited affinity endothelial cells. HA-coated possess core–shell structure of self-assembled biodegradable poly(l-lysine)-b-poly(lactic acid) AB-diblock copolymer (PLys+-b-PLLA), its exterior coated with HA...

"Molecular Cybernetics" is an emerging research field aiming the development of "Chemical AI", artificial intelligence with memory and learning capabilities based on molecular communication. It originated from Robotics", which studies systems that comprise three basic elements robots; Sensing, Planning, Acting. Development Amoeba-type robot (unicellular cell,) motivated construction multicellular cell mimicking nerve systems. The major challenges in cybernetics are communication over two...

Even in the presence of a large excess ZnII ions, sequence-selective RNA hydrolysis is achieved by DNA conjugates involving dinuclear complex (shown schematically). This because cooperation two ions essential for scission.

We describe the synthesis of a hybrid DNA/organic macrocycle that is prepared by formation an amide linkage across one full turn DNA. Formation catenane proved crossed rather than running along phosphodiester backbone contour. The product, doubly tailed catenane, contains 5'- and 3'-termini can be functionalized further or used to incorporate structure into other DNA assemblies.

Multiple enzymes were orthogonally arrayed in a single-molecule manner on waffle-like DNA origami scaffolds with size-tunable wells.

Abstract A new class of hydrogels utilizing DNA (DNA quadruplex gel) has been constructed by directly and symmetrically coupling deoxynucleotide phosphoramidite monomers to the ends polyethylene glycols (PEGs) in liquid phase, using resulting DNA‐PEG‐DNA triblock copolymers as macromonomers. Elongation merely four deoxyguanosine residues on PEG, which produces typically ≈10 grams desired DNA‐PEG conjugates one synthesis, resulted intelligent biodegradable formation, are responsive various...