A5062215861- Antibiotic Resistance in Bacteria
- Evolution and Genetic Dynamics
- Bacterial Genetics and Biotechnology
- Antibiotics Pharmacokinetics and Efficacy
- CRISPR and Genetic Engineering
- Evolutionary Game Theory and Cooperation
- Biomedical and Engineering Education
- Analytical Chemistry and Chromatography
- Gut microbiota and health
- Antibiotic Use and Resistance
- Biotechnology and Related Fields
- Plant and animal studies
- DNA Repair Mechanisms
- Bacteriophages and microbial interactions
- Epigenetics and DNA Methylation
- Legume Nitrogen Fixing Symbiosis
- Vibrio bacteria research studies
- Microbial Natural Products and Biosynthesis
- Escherichia coli research studies
- Water Systems and Optimization
- Cancer therapeutics and mechanisms
- Aquaculture disease management and microbiota
- Mathematical and Theoretical Epidemiology and Ecology Models
- Advanced biosensing and bioanalysis techniques
- Pharmaceutical and Antibiotic Environmental Impacts
European Commission
2025
Centre National de la Recherche Scientifique
2006-2024
Université Paris Cité
2011-2024
Inserm
2013-2024
Institut Cochin
2022-2024
Broad Institute
2017-2022
Massachusetts Institute of Technology
2015-2022
Evolva (Switzerland)
2022
Sorbonne Paris Cité
2013-2021
Délégation Paris 5
2013-2018
Bacteriostatic and bactericidal antibiotic treatments result in two fundamentally different phenotypic outcomes--the inhibition of bacterial growth or, alternatively, cell death. Most antibiotics inhibit processes that are major consumers cellular energy output, suggesting treatment may have important downstream consequences on metabolism. We hypothesized the specific metabolic effects bacteriostatic contribute to their overall efficacy. leveraged opposing phenotypes drugs combination...
Regardless of their targets and modes action, subinhibitory concentrations antibiotics can have an impact on cell physiology trigger a large variety cellular responses in different bacterial species. Subinhibitory β-lactam cause reactive oxygen species production induce PolIV-dependent mutagenesis Escherichia coli. Here we show that the RpoS regulon. RpoS-regulon induction is required for because it diminishes control DNA-replication fidelity by depleting MutS E. coli, Vibrio cholerae...
Physiologic and environmental factors can modulate antibiotic activity thus pose a significant challenge to treatment. The quinolone class of antibiotics, which targets bacterial topoisomerases, fails kill bacteria that have grown high density; however, the mechanistic basis for this persistence is unclear. Here, we show exhaustion metabolic inputs couple carbon catabolism oxidative phosphorylation primary cause growth phase-dependent antibiotics. Supplementation stationary-phase cultures...
β-Lactam antibiotics disrupt the assembly of peptidoglycan (PG) within bacterial cell wall by inhibiting enzymatic activity penicillin-binding proteins (PBPs). It was recently shown that β-lactam treatment initializes a futile cycle PG synthesis and degradation, highlighting major gaps in our understanding lethal effects PBP inhibition antibiotics. Here, we assess downstream metabolic consequences Escherichia coli with mecillinam show lethality from PBP2 is specific consequence toxic shifts...
Considerable attention has focused on how selection dispersal and other core life-history strategies (reproductive effort, survival ability, colonization capacity) may lead to so-called syndromes. Studies genetic variation in these syndromes within species could importantly increase our understanding of their evolution, by revealing whether traits co-vary across lineages the manner predicted theoretical models, stimulating further hypotheses for experimental testing. Yet such studies remain...
Host-parasite coevolution is often described as a process of reciprocal adaptation and counter adaptation, driven by frequency-dependent selection. This requires that different parasite genotypes perform differently on host genotypes. Such genotype-by-genotype interactions arise if to one (or parasite) genotype reduces performance others. These direct costs can maintain genetic polymorphism generate geographic patterns local or adaptation. Fixation all-resistant all-infective) further...
Though predation, productivity (nutrient richness), spatial heterogeneity, and disturbance regimes are known to influence species diversity, interactions between these factors remain largely unknown. Predation has been shown interact with but few experimental studies have focused on how predation prey diversity. We used theory experiments investigate diversification of Pseudomonas fluorescens by manipulating both (presence or absence Bdellovibrio bacteriovorus) (frequency intensity...
Antibiotics of the β-lactam (penicillin) family inactivate target enzymes called D,D-transpeptidases or penicillin-binding proteins (PBPs) that catalyze last cross-linking step peptidoglycan synthesis. The resulting net-like macromolecule is essential component bacterial cell walls sustains osmotic pressure cytoplasm. In Escherichia coli , bypass PBPs by YcbB L,D-transpeptidase leads to resistance these drugs. We developed a new method based on heavy isotope labeling and mass spectrometry...
Summary Bacterial resistance to bacteriophages (phages) relies on two primary strategies: preventing phage attachment and blocking post-attachment steps. These latter mechanisms are mediated by defence systems, including DNA-degrading systems such as Restriction- Modification (RM) CRISPR-Cas, well abortive infection that induce cell death or dormancy. Computational studies suggest bacterial genomes encode several which may act synergistically enhance resistance. However, the regulation,...
The contribution of toxin-antitoxin systems to the persistence bacteria antibiotics has been intensively studied. This is also case with E. coli TisB/IstR system, but TisB turned out be not as straightforward anticipated.
Though predation, productivity (nutrient richness), spatial heterogeneity, and disturbance regimes are known to influence species diversity, interactions between these factors remain largely unknown. Predation has been shown interact with but few experimental studies have focused on how predation prey diversity. We used theory experiments investigate diversification of Pseudomonas fluorescens by manipulating both (presence or absence Bdellovibrio bacteriovorus) (frequency intensity...
Escherichia coli SOS functions constitute a multifaceted response to DNA damage. We undertook study the role of yafP, gene with unknown function. yafP is part an operon also containing dinB coding for Polymerase IV (PolIV). Our phylogenetic analysis showed that content this variable but and genes are conserved in majority E. natural isolates. Therefore, we studied if these proteins functionally linked. Using murine septicaemia model, YafP activity reduced bacterial fitness absence PolIV....
Evolution is often an obstacle to the engineering of stable biological systems due selection mutations inactivating costly gene circuits. Gene overlaps induce important constraints on sequences and their evolution. We show that these can be harnessed increase stability genes by purging loss-of-function mutations. combine computational synthetic biology approaches rationally design overlapping reading frame expressing essential within existing protect. Our algorithm succeeded in creating...
Host-parasite coevolution is often described as a process of reciprocal adaptation and counter adaptation, driven by frequency-dependent selection. This requires that different parasite genotypes perform differently on host genotypes. Such genotype-by-genotype interactions arise if to one (or parasite) genotype reduces performance others. These direct costs can maintain genetic polymorphism generate geographic patterns local or adaptation. Fixation all-resistant all-infective) further...
Abstract Evolution is often an obstacle to the engineering of stable biological systems due selection mutations inactivating costly gene circuits. Gene overlaps induce important constraints on sequences and their evolution. We show that these can be harnessed increase stability synthetic circuits by purging loss-of-function mutations. combine computational biology approaches rationally design overlapping reading frame expressing essential within existing protect. Our algorithm succeeded in...