A5089483773- RNA Interference and Gene Delivery
- Advanced Drug Delivery Systems
- Advanced biosensing and bioanalysis techniques
- Silk-based biomaterials and applications
- Helminth infection and control
- Mycotoxins in Agriculture and Food
- Polysaccharides and Plant Cell Walls
- Bee Products Chemical Analysis
- Insect Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Transgenic Plants and Applications
- Proteins in Food Systems
- Immunotherapy and Immune Responses
- Analytical Chemistry and Sensors
- Parasite Biology and Host Interactions
- Graphene and Nanomaterials Applications
- Essential Oils and Antimicrobial Activity
- Parasites and Host Interactions
- Nematode management and characterization studies
- Studies on Chitinases and Chitosanases
- HIV-related health complications and treatments
- Viral Infections and Immunology Research
- Allelopathy and phytotoxic interactions
- Macrophage Migration Inhibitory Factor
- Polymer Surface Interaction Studies
University of Massachusetts Chan Medical School
2016-2025
Worcester Polytechnic Institute
2006-2008
Boston University
1979
Nonviral gene delivery technologies have been developed using layer-by-layer self-assembly of nanomaterials held together by electrostatic interactions in order to provide nanoparticulate materials that protect and deliver DNA cells. Here we report a new technology based on the situ synthesis nanoparticles caged within hollow yeast cell wall particles (YCWP). YCWP protection facilitate oral systemic receptor-targeted payloads phagocytic The inside consist core tRNA/polyethylenimine (PEI)...
Glucan particles (GPs) are hollow, porous 2-4 μm microspheres derived from the cell walls of Baker's yeast (Saccharomyces cerevisiae). The 1,3-β-glucan outer shell provides for receptor-mediated uptake by phagocytic cells expressing β-glucan receptors. GPs have been used macrophage-targeted delivery soluble payloads (DNA, siRNA, protein, and small molecules) encapsulated inside hollow via core polyplex layer-by-layer (LbL) synthetic strategies. In this communication, we report incorporation...
Phagocytic macrophages and dendritic cells are desirable targets for potential RNAi (RNA interference) therapeutics because they often mediate pathogenic inflammation autoimmune responses. We recently engineered a complex 5 component glucan-based encapsulation system siRNA (small interfering RNA) delivery to phagocytes. In experiments designed simplify this original formulation, we discovered that the amphipathic peptide Endo-Porter forms stable nanocomplexes with can potent gene silencing...
Staphylococcus epidermidis is among the most commonly isolated microbes from medical implant infections, particularly in colonization of blood-contacting devices. We explored relationships between surface wettability and root-mean-square roughness (Rq) on microbial adhesive strength to a substrate. Molecular-level interactions S. variety chemically texturally distinct model substrata were characterized using cellular probe atomic force microscopy (AFM). Substrata included gold, aliphatic...
Glucan particles (GPs) are 2–4 mm spherical, hollow, porous shells extracted from Baker’s yeast, Saccharomyces cerevisae. The surface of the GPs is composed primarily 1,3-b-glucan and efficiently phagocytosed via receptor-mediated cell uptake by macrophages, phagocytic cells expressing glucan receptors. hollow cavity allows for efficient absorption encapsulation payload molecules. Rifampicin (Rif), a drug used in tuberculosis treatment, was encapsulated precipitation trapped using calcium...
ABSTRACT Simpler, safer, and faster chemotherapeutic regimens for tuberculosis other respiratory mycobacterial infections are an urgent need. Many current therapies suffer from suboptimal drug exposure dose-limiting systemic adverse effects, challenges that could be addressed via controlled delivery of drugs to the primary site infection. We sought address this need by designing a flexible formulation platform targets lung macrophages concentrate at infectious foci. Our approach is based on...
Glucan particles (GPs) are hollow, porous 3–5 μ m microspheres derived from the cell walls of Baker’s yeast ( Saccharomyces cerevisiae). The 1,3- β -glucan outer shell provides for receptor-mediated uptake by phagocytic cells expressing receptors. GPs have been used macrophage-targeted delivery a wide range payloads (DNA, siRNA, protein, small molecules, and nanoparticles) encapsulated inside hollow or bound to surface chemically derivatized GPs. Gallium nanoparticles proposed as an...
Invasive carcinoma of the larynx was induced in 36.8% inbred Syrian golden hamsters from strain B10® 15.16, susceptible to this type cancer when exposed smoke reference filter cigarettes for 59–80 weeks. Nearly half animals (47.4%) showed laryngeal cancer, including noninvasive and situ, which occurred with daily exposures (twice a day 12 min each time, 27 sec out min) 7 days week at concentrations 22%. When concentration reduced 11%, number lesions proportionately. portion tobacco replaced...
Soil-transmitted nematodes (STN) infect 1–2 billion of the poorest people worldwide. Only benzimidazoles are currently used in mass drug administration, with many instances reduced activity. Terpenes a class compounds anthelmintic Thymol, natural monoterpene phenol, was to help eradicate hookworms U.S. South circa 1910. However, use terpenes as anthelmintics discontinued because adverse side effects associated high doses and premature stomach absorption. Furthermore, dose–response activity...
Addressing the growing problem of antifungal resistance in medicine and agriculture requires development new drugs strategies to preserve efficacy existing fungicides. One approach is utilize delivery technologies. Yeast particles (YPs) are 3–5 µm porous, hollow microspheres, a byproduct food-grade Saccharomyces cerevisiae yeast extract manufacturing processes an efficient flexible drug platform. Here, we report use YPs for encapsulation tetraconazole (TET) prothioconazole (PRO) with high...
Glucan particles (GPs) are hollow, porous 3-5 µm microspheres derived from the cell walls of Baker's yeast (Saccharomyces cerevisiae). Their 1,3-β-glucan outer shell allows for receptor-mediated uptake by macrophages and other phagocytic innate immune cells expressing β-glucan receptors. GPs have been used targeted delivery a wide range payloads, including vaccines nanoparticles, encapsulated inside hollow cavity GPs. In this paper, we describe methods to prepare GP-encapsulated nickel...
Self-assembled monolayers (SAMs) of 21-(16-mercaptohexadecan-1-oyl)-4,7,13,16-tetraoxa-1,10,21-triazabicyclo[8.8.5]tricosane-19,23-dione were prepared on gold. Characterization the SAMs was carried out by sessile drop contact angle, ellipsometry, grazing angle FT-IR spectroscopy, and electrochemical techniques. The cation recognition properties SAM studied cyclic voltammetry impedance spectroscopy. films show moderate selectivity for detection Li+ ions in solution over K+ Na+, with values...
Gastrointestinal nematodes (GINs) of humans, e.g., hookworms, negatively impact childhood growth, cognition, nutrition, educational attainment, income, productivity, and pregnancy. Hundreds millions people are targeted with mass drug administration (MDA) donated benzimidazole anthelmintics. However, efficacy against GINs is suboptimal, reduced/low has been seen. Developing an anthelmintic for human MDA daunting: it must be safe, effective, inexpensive, stable without a cold chain, massively...
Precisely engineering the surface chemistry of biomaterials to modulate adsorption and functionality biochemical signaling molecules that direct cellular functions is critical in development tissue engineered scaffolds. Specifically, this study describes use functionalized self-assembled monolayers (SAMs) as a model system assess effects biomaterial properties on controlling fibronectin (FN) conformation concentration well keratinocyte function. By systematically analyzing FN at low...
Glucan particles (GPs) are hollow, porous 3–4 μm microspheres derived from the cell walls of Baker’s yeast (Saccharomyces cerevisiae). The β-1,3-D glucan outer shell GPs provides for receptor-mediated uptake by phagocytic cells expressing β-glucan receptors. have been used efficient encapsulation different types payloads (DNA, siRNA, proteins, antigens, small molecules), and these delivered in vivo a variety routes including oral delivery. It is known that transported across intestinal...
Terpenes are naturally occurring compounds produced by plants that of great commercial interest in the food, agricultural, cosmetic, and pharmaceutical industries due to their broad spectra antibacterial, antifungal, anthelmintic, membrane permeation enhancement, antioxidant biological activities. Applications terpenes often limited volatility need for surfactants or alcohols produce stable, soluble (non-precipitated) products. Yeast particles (YPs) hollow, porous microspheres have been used...
This study aimed to assess the protective effect of encapsulating humic acid-iron complexed nanoparticles (HA-Fe NPs) inside glucanmannan lipid particles (GMLPs) extracted from yeast cell wall against aflatoxin B (AFB1 ) toxicity in vivo. Four groups male Sprague-Dawley rats were treated orally for 2 weeks included control group, AFB1 group (80 µg/kg b.w); GMLP/HA-Fe NPs (0.5 mg/kg b.w), and with plus NPs. GMLPs are empty 3-4 micron permeable microspheres that provide an efficient system...
Bacillus thuringiensis (Bt) is a Gram-positive soil bacterium that widely and safely applied in the environment as an insecticide for combatting insect pests damage crops or are disease vectors. Dominant active ingredients made by Bt insect-killing crystal (Cry) proteins released crystalline inclusions upon bacterial sporulation. Some Cry proteins, e.g., Cry5B (formally Cry5Ba1), target nematodes (roundworms) show exceptional promise anthelmintics (cures parasitic nematode diseases). We have...