A5002917546- Carbohydrate Chemistry and Synthesis
- Genomics, phytochemicals, and oxidative stress
- Sphingolipid Metabolism and Signaling
- Natural product bioactivities and synthesis
- Synthetic Organic Chemistry Methods
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Synthesis and biological activity
- Bioactive Compounds and Antitumor Agents
- Cancer therapeutics and mechanisms
- Lysosomal Storage Disorders Research
- Berberine and alkaloids research
- Autophagy in Disease and Therapy
- Phytochemical compounds biological activities
- Synthesis of Organic Compounds
- Plant-derived Lignans Synthesis and Bioactivity
- Crystallography and molecular interactions
- Retinoids in leukemia and cellular processes
- Cell death mechanisms and regulation
- Garlic and Onion Studies
- Free Radicals and Antioxidants
- Invertebrate Immune Response Mechanisms
- Synthesis and Biological Evaluation
- Nitrogen and Sulfur Effects on Brassica
- Click Chemistry and Applications
University of Pavol Jozef Šafárik
2015-2024
Institute of Pharmacology
2008-2024
Faculty (United Kingdom)
2019
Technical University of Košice
2019
King's College London
2012-2017
University of Veterinary Medicine Vienna
2015
Infections disturb metabolic homeostasis in many contexts, but the underlying connections are not completely understood. To address this, we use paired genetic and computational screens Drosophila to identify transcriptional regulators of immunity pathology their associated target genes physiologies. We show that Mef2 is required fat body for anabolic function immune response. Using biochemical approaches, find MEF2 phosphorylated at a conserved site healthy flies promotes expression...
Abstract Mycobacterium tuberculosis remains a global threat to human health, yet the molecular mechanisms regulating immunity remain poorly understood. Cytokines can promote or inhibit mycobacterial survival inside macrophages and underlying represent potential targets for host-directed therapies. Here we show that cytokine-STAT signalling promotes within by deregulating lipid droplets via ATG2 repression. In Drosophila infected with marinum , mycobacterium-induced STAT activity triggered...
This study was designed to examine the in vitro antiproliferative effect of brassinin and its derivatives on human cancer cell lines. Among seven tested compounds, homobrassinin (K1; N-[2-(indol-3-yl)ethyl]-S-methyldithiocarbamate) exhibited most potent activity with IC50 = 8.0 μM colorectal Caco2 cells selected for further studies. The flow cytometric analysis revealed a K1-induced increase G2/M phase associated dysregulation α-tubulin, α1-tubulin β5-tubulin expression. These findings...
Despite the decreasing trend in mortality from colorectal cancer, this disease still remains third most common cause of death cancer. In present study, we investigated antiproliferative and pro-apoptotic effects (2S,3S,4R)-2-tridecylpyrrolidine-3,4-diol hydrochloride on colon cancer cells (Caco-2 HCT116). The effect IC50 values were determined by MTT BrdU assays. Flow cytometry, qRT-PCR Western blot used to study cellular molecular mechanisms involved induction apoptotic pathways. Colon cell...
Non-small cell lung cancer has one of the highest mortality rates among cancer-suffering patients. It is well known that unwanted psychotropic effects cannabinoids (CBs) are mediated via CB 1 receptor (R), and selective targeting 2 R would thus avoid side in treatment. Therefore, aim our study was to evaluate effect agonist, JWH-133, on A549 cells (non-small cancer) human umbilical vein endothelial (HUVECs). Cytotoxicity assay DNA fragmentation were employed influence JWH-133...
Colorectal cancer is the third most common in world, with 1.2 million new cases annually. Chalcones are secondary metabolite precursors of flavonoids that exhibit diverse biological activities, including antioxidant and antitumor activities. The aim this study was to investigate antiproliferative effect synthetic chalcone derivatives on HCT116 cells. (E)-2-(2',4'-dimethoxybenzylidene)-1-tetralone (Q705) found be active (IC50 = 3.44 ± 0.25 μM). Based these results, compound chosen for further...
In our study we used quercetin (3,3´,4´,5,7-pentahydroxyflavone) as the reference standard to compare antiproliferative and antiangiogenic effects of chrysin (5,7-dihydroxyflavone) 3-hydroxyflavone.Our data indicates that 3-hydroxyflavone showed significantly higher cytotoxic effect than quercetin.These tested agents decreased cell survival with efficacy 65-85% at concentration 100 µmol/l for HUVEC, lung carcinoma leukemic cells being most sensitive.Cell cycle analysis might affect Jurkat by...
<i>Background:</i> Several indole phytoalexins exhibit antiproliferative and/or cancer chemopreventive properties in vitro. However, the potency and selectivity of their anticancer effects were reported to be relatively weak. In order improve activity natural phytoalexin 1-methoxyspirobrassinol, its new 2-amino analogues synthesized evaluated. <i>Methods:</i><i>Cis</i>-1-Boc-, <i>trans</i>-1-Boc-, <i>cis</i>-1-methoxy-...
Estrogen deprivation is considered responsible for many age-related processes, including poor wound healing. Guided by previous observations that estradiol accelerates re‑epithelialization through estrogen receptor (ER)‑β, in the present study, we examined whether selective ER agonists [4,4',4''-(4-propyl [1H] pyrazole-1,3,5-triyl)‑trisphenol (PPT), ER‑α agonist; 2,3-bis(4-hydroxyphenyl)-propionitrile (DPN), ER‑β agonist] affect expression of basic proliferation and differentiation markers...
This study examined the antiproliferative effects of β-escin (E) in cancer cells. The showed that E inhibited cells growth a dose-dependent manner. flow cytometric analysis revealed an escin-induced increase sub-G1 DNA content, which is considered to be marker apoptosis. Apoptosis was also confirmed by annexin V staining and fragmentation assay. These were associated with increased generation reactive oxygen species (ROS), caspase-3 activation decreased mitochondrial membrane potential...
Abstract Mistletoe preparations are frequently used by cancer patients because of their ability to stimulate the immunity and improve quality life. Moreover mistletoe its active substances (especially lectins) possess cytotoxic effect on various cell lines. However, only little is known about interaction with anticancer drugs. Therefore apoptosis‐inducing effects aqueous extract (VA) doxorubicin (DOXO) were investigated in Jurkat cells. The results show that VA as well DOXO exert cells a...