One of the major social and public health problems in world is adolescent pregnancy. Adolescent pregnancy strongly associated to less favorable results for both mother newborn. We conducted this research ascertain impact teenage age on neonatal outcomes also observed lifestyles pregnant girls. a study 2434 mothers aged ≤19 years (n = 294) or 20-34 2140) who gave birth 2019-2020 at Department Gynaecology Obstetrics Louis Pasteur University Hospital Košice. The data newborn infants have been...

This study was designed to examine the in vitro antiproliferative effect of brassinin and its derivatives on human cancer cell lines. Among seven tested compounds, homobrassinin (K1; N-[2-(indol-3-yl)ethyl]-S-methyldithiocarbamate) exhibited most potent activity with IC50 = 8.0 μM colorectal Caco2 cells selected for further studies. The flow cytometric analysis revealed a K1-induced increase G2/M phase associated dysregulation α-tubulin, α1-tubulin β5-tubulin expression. These findings...

Objectives: Low birth weight (LBW) is one of the major factors affecting child morbidity and mortality worldwide. Every day, approximately 800 women die from causes related to pregnancy childbirth Maternal ill health increases risk LBW. This study seeks investigate determinants incidence Methods: was conducted based on medical records mothers their 1,946 infants born in 2016-2019 at Department Gynaecology Obstetrics Louis Pasteur University Hospital Košice. Data newborn were obtained Reports...

In the present study we evaluated anti-angiogenic activities of β-escin (the major active compound Aesculus hippocastanum L. seeds). Human umbilical-vein endothelial cells (HUVECs) were used as an in vitro model for studying molecular mechanism underlying effect β-escin. We investigated effects on proliferation, migration, and tube formation HUVECs vivo activity was a chick chorioallantoic membrane (CAM) angiogenesis assay. Moreover, gene expressions determined by RT2 ProfilerTM human PCR...

Abstract Hypericin is the most powerful naturally occurring photosensitizer and as such there renaissant interest in potentials of this compound for anticancer photodynamic therapy (PDT). The purpose study was to investigate hypericin‐mediated effects on normal human umbilical endothelial cells (HUVECs) comparison with cancer glioma cell lines U‐87 MG U‐373 MG, vitro conditions. data suggest that well are sensitive only photoactivated hypericin. inhibitory hypericin did not differ compared...

Objective: The aim of the cross-sectional study was to evaluate cardiovascular risk factors in group medical students with gender, overweight and obesity categorisation. Methods: Cross-sectional included 364 students, 207 females 157 males. We investigated anthropometric parameters, BMI, body fat percentage, WHR (waist-hip ratio), TCH (total cholesterol) LDL-CH (LDL-cholesterol), SBP DBP (systolic diastolic blood pressure). participants also completed questionnaires socio-demographic...

This study analyses the differences in birth outcomes between Roma and non-Roma mothers investigates potential causes of such differences.We conducted a retrospective cohort 1,989 799 who gave 2014 2015 at Gynaecology Obstetrics Clinic Louis Pasteur University Hospital Košice. Data on new-born infants have been obtained from book reports childbirth. For low weight we considered weighing less than 2,500 grams as for premature referred to childbirth before pregnancy week 37. The file was split...

Peripheral blood CD8+ T lymphocytes play a crucial role in cell-mediated immunity and tumor-related immune responses breast cancer. In this study, label-free quantification analysis gene set enrichment (GSEA) of the peripheral benign patients with different cancer (BC) subtypes, i.e., luminal A, B, triple-negative (TNBC), were performed using nano-UHPLC Orbitrap mass spectrometry. Differential protein expression revealed significant downregulation (log2 FC ≥ 0.38 or ≤-0.38, adj. p < 0.05),...

Despite advances in the genomic classification of breast cancer, current clinical tests and treatment decisions are commonly based on protein-level information. Nowadays cancer selection is immunohistochemical (IHC) determination four protein biomarkers: Estrogen Receptor 1 (ESR1), Progesterone (PGR), Human Epidermal Growth Factor 2 (HER2), proliferation marker Ki-67. The prognostic correlation tumor-infiltrating T cells has been widely studied but B have not received so much attention. We...

Endometrial cancer is becoming increasingly common, highlighting the need for improved diagnostic methods that are both effective and non-invasive. This study investigates use of urinary fluorescence spectroscopy as a potential tool endometrial cancer. Urine samples were collected from patients (n = 77), with benign uterine tumors 23), control gynecological attending regular checkups or follow-ups 96). These analyzed using synchronous to measure total fluorescent metabolome profile, specific...

Pregnancy at advanced maternal age has become more common over the last decades. Therefore, study aimed to describe characteristics and perinatal outcomes of women giving birth very age. We conducted a retrospective cohort 2,300 singleton births that occurred in 2020-2021 Department Gynaecology Obstetrics Louis Pasteur University Hospital Košice. The control (age 20-34 years), (35-39 (≥ 40 years) groups included 1,851, 382, 67 women, respectively. Exclusion criteria were multiple...

Abstract Background The aim of this study was to explore the personal views female gynecologists regarding management POP with a particular focus on issue uterine sparing surgery. Methods A questionnaire based survey practicing in Czech Republic, Slovenia and Slovakia. Results total 140 from 81 units responded our questionnaire. majority respondents stated they would rely urogynecologist aid them their choice options. most preferred options for were sacrocolpopexy physiotherapy. Almost 2/3...

The aim of our study was to establish the predictive value pelvic floor muscle morphometry using 3D/4D ultrasound in relation success training (PFMT) for 12 weeks women with stress urinary incontinence (SUI). A total 86 SUI from regional gynaecological and urological outpatient clinics were enrolled on this cross-sectional study. symptoms assessed by International Consultation Incontinence Questionnaire (ICIQ-UI SF). Pelvic function evaluated a perineometer. (PFMM) size urogenital hiatus (HA...

Flavin7 (F7) is a nutritional supplement often taken by cancer patients in Central Europe during chemo- and radiation therapy. In this study, investigation of the antiproliferative antiangiogenic activities were performed. showed activity Jurkat as well HeLa cells. It significantly reduced growth both cell lines at doses 200 μg/ml to 20 (p&lt;0.001 p&lt;0.01, respectively). F7-treated cells we found significant increase fraction with sub-G0/G1 DNA content, which considered be marker...

BACKGROUND: A hundred years ago, scientists believed that amniotic fl uid is a yellowish hypotonic mixture of foetal urine and maternal transudate with peculiar odour.Current knowledge shows it represents dynamic, complex inorganic organic compounds.OBJECTIVES: Despite modern technological procedures, information still lacking about the composition properties uid.We focused on dynamics selected physical chemical increasing gestational week.METHODS: The physicochemical characteristics 89...

Health characteristics associated with uric acid (UA) in the Roma minority remain less well known. The study sought to determine ethnicity- and sex-specific associations of serum UA health factors Eastern Slovakian non-Roma populations.

The aetiology, differential diagnosis and management strategies of the foetal spleen affected with a cystic lesion are discussed. In current literature, there very few reports that relate to antenatally diagnosed splenic cyst. Our study presents 3 case were first suspected due anisoechogenic structures detected during routine ultrasonographic examination at 27th, 31st 34th weeks gestation. All cases further characterized by lack pathological power Doppler findings inside around lesions,...

Abstract Endometriosis is a chronic inflammatory disease which increasingly affects young women under 35 years of age and leads to subfertility even infertility. Analysis the cytotoxic effect zinc(II) niflumato complex with neocuproine ([Zn( neo )( nif ) 2 ] or Zn-Nif) on immortalized human endometriotic cell line (12Z) control endometrial stromal (hTERT) was performed using xCELLigence technology for approximately 72 h following treatment Zn-Nif as well viability Trypan Blue Assay. 12Z...

The increased interest in assisted reproduction through vitro fertilization (IVF) leads to an urgent need identify biomarkers that reliably highly predict the success of pregnancy. Despite advances diagnostics, treatment, and IVF approaches, 30% rate seems insurmountable. Idiopathic infertility does not have any explanation for failure especially when a patient is treated with healthy competitive embryo capable implantation development. Since appropriate intercellular communication essential...