A5072678185- Gut microbiota and health
- Immunotherapy and Immune Responses
- Pediatric Pain Management Techniques
- Fish biology, ecology, and behavior
- Machine Learning in Bioinformatics
- Fish Biology and Ecology Studies
- interferon and immune responses
- Clostridium difficile and Clostridium perfringens research
- MicroRNA in disease regulation
- RNA Research and Splicing
- Burn Injury Management and Outcomes
- Gastrointestinal motility and disorders
- Wound Healing and Treatments
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Tryptophan and brain disorders
- Genetic and phenotypic traits in livestock
- Food Allergy and Anaphylaxis Research
- Research Data Management Practices
- Insect-Plant Interactions and Control
- Autophagy in Disease and Therapy
- Plant and animal studies
- Vibrio bacteria research studies
- Genetic diversity and population structure
- Genomics and Phylogenetic Studies
Earlham Institute
2018-2024
Norwich Research Park
2004-2024
Bridgeport Hospital
2022
University of California Davis Medical Center
2022
Massachusetts General Hospital
2022
TD Bank Group
2022
University of California, Davis
2022
Shriners Hospitals for Children - Northern California
2022
Johns Hopkins University
2022
Quadram Institute
2004-2018
The gut-brain axis and the intestinal microbiota are emerging as key players in health disease. Shifts composition affect a variety of systems; however, evidence their direct impact on cognitive functions is still lacking. We tested whether faecal transplant (FMT) from aged donor mice into young adult recipients altered hippocampus, an area central nervous system (CNS) known to be affected by ageing process related functions.Young were transplanted with either or age-matched mice. Following...
The physical and immunological properties of the human intestinal epithelial barrier in aging are largely unknown. Ileal biopsies from young (7-12 years), adult (20-40 years) (67-77 individuals not showing symptoms gastrointestinal (GI) pathologies were used to assess levels inflammatory cytokines, integrity cytokine production response microbial challenges. Increased expression interleukin (IL)-6, but interferon (IFN)γ, tumour necrosis factor (TNF)-α IL-1β was observed during aging; further...
Influenza A virus (IAV) and SARS-CoV-2 (COVID-19) cause pandemic infections where cytokine storm syndrome lung inflammation lead to high mortality. Given the social economic cost of respiratory viruses, there is an urgent need understand how airways defend against infection. Here we use mice lacking WD linker domains ATG16L1 demonstrate that ATG16L1-dependent targeting LC3 single-membrane, non-autophagosome compartments - referred as non-canonical autophagy protects from lethal IAV Mice with...
Abstract Cichlid fishes of the genus Oreochromis (tilapia) are among most important fish for inland capture fisheries and global aquaculture. Deliberate introductions non-native species improvement accidental escapees from farms have resulted in admixture with indigenous species. Such hybridization may be detrimental to native biodiversity, potentially leading genomic homogenization populations loss genetic material associated local adaptation. By contrast, introgression fuel diversification...
Alternative splicing is a key mechanism underlying cellular differentiation and driver of complexity in mammalian neuronal tissues. However, understanding which isoforms are differentially used or expressed how this affects remains unclear. Long read sequencing allows full-length transcript recovery quantification, enabling transcript-level analysis alternative processes these change with cell state. Here, we utilise Oxford Nanopore Technologies to produce custom annotation well-studied...
Abstract During Salmonella Typhimurium infection, intestinal CX3CR1+ cells can either extend transepithelial cellular processes to sample luminal bacteria or, very early after migrate into the lumen capture bacteria. However, until now, biological relevance of intraluminal migration remained be determined. We addressed this by using a combination mouse strains differing in their ability carry out CX3CR1-mediated sampling and migration. observed that number S. traversing epithelium did not...
Abstract It has been shown previously that certain bacteria rapidly (3 h) up-regulated in vivo microfold cell (M cell)-mediated transport of Ag across the follicle-associated epithelium intestinal Peyer’s patch. Our aim was to determine whether soluble mediators secreted following host-bacteria interaction were involved this event. A combination proteomics and immunohistochemical analyses used identify molecules produced gut response bacterial challenge vivo; their effects then tested on...
Specific molecular-receptor interactions with gut epithelium cells are important in understanding bioactivity of food components and drugs, binding commensal microflora, attachment initiation defense mechanisms against pathogenic bacteria for development targeted delivery systems to the gut. However, methods probing such lacking. Methodology has been developed validated measure specific on living human colorectal cancer as vitro models epithelium. Atomic force microscopy (AFM) was used...
Summary Dendritic cells (DCs) are important in the regulation of immune responses and it has been proposed that these play an role asthma; however, their food allergy is still largely unknown. Our aim was to study specific immunoglobulin E (IgE) G (IgG) naïve recipients following adoptive transfer myeloid DCs from allergic control mice. The phenotypic features lymphokine production were also investigated. CD11c + /hi B220 − isolated spleen Peyer's patches (PP) cow's milk (CM) mice...
Abstract Background The gut-brain axis and the intestinal microbiota are emerging as key players in health disease. Shifts composition affect a variety of systems, however, evidence their direct impact on cognitive functions is still lacking. We tested whether faecal transplant (FMT) from aged donor mice into young adult recipients affected hippocampus, an area central nervous system (CNS) known to be by ageing process, related functions. Methods Findings Young were transplanted with either...
East African lake cichlids are one of the most impressive examples an adaptive radiation. Independently in Lake Victoria, Tanganyika, and Malawi, several hundreds species arose within last 10 million to 100,000 years. Whereas analyses focused on nucleotide substitutions across investigate genetic bases this explosive radiation, date, no study has investigated contribution structural variants (SVs) evolution traits three Great Lakes Africa.
Interaction between intestinal epithelial cells (IECs) and the underlying immune systems is critical for maintaining homeostasis mounting appropriate responses. We have previously showed that T helper type 1 (TH1) cytokine IL-12 plays a key role in delicate immunological balance gut lack of levels had important consequences health disease, particularly with regard to food allergy. Here, we sought understand regulation lymphoepithelial cross talk how this interaction affects responses locally...
Summary Respiratory viruses such as influenza A virus (IAV) and SARS-CoV-2 (Covid-19) cause pandemic infections where cytokine storm syndrome, lung inflammation pneumonia lead to high mortality. Given the social economic cost of these viruses, there is an urgent need for a comprehensive understanding how airways defend against infection. Viruses entering cells by endocytosis are killed when delivered lysosomes degradation. Lysosome delivery facilitated non-canonical autophagy pathways that...
We present a reference genome assembly from an individual male Violet Carpenter Bee (Xylocopa violacea, Linnaeus, 1758). The is 1.02 gigabases in span. 48% of the scaffolded into 17 pseudo-chromosomal units. mitochondrial has also been assembled and 21.8 kilobases length. highly repetitive, likely representing heterochromatic architecture expected bees genus Xylocopa. use evidence-based methodology to annotate 10,152 high confidence coding genes. This was sequenced as part pilot project...
We present a reference genome assembly from an individual male Violet Carpenter Bee (Xylocopa violacea, Linnaeus 1758). The is 1.02 gigabases in span. 48% of the scaffolded into 17 pseudo-chromosomal units. mitochondrial has also been assembled and 21.8 kilobases length. highly repetitive, likely representing heterochromatic architecture expected bees genus Xylocopa. use evidence-based methodology to annotate 10,152 high confidence coding genes. This was sequenced as part pilot project...
ABSTRACT African Lakes Cichlids are one of the most impressive example adaptive radiation. Independently in Lake Victoria, Tanganyika, and Malawi, several hundreds species arose within last 10 million to 100,000 years. Whereas analyses focused on nucleotide substitutions across investigate genetic bases this explosive radiation, date, no study has investigated contribution structural variants (SVs) speciation events (through a reduction gene flow) adaptation different ecological niches....
The Nile tilapia (Oreochromis niloticus) accounts for ∼9% of global freshwater finfish production however, extreme cold weather and decreasing resources has created the need to develop resilient strains. By determining genetic bases aquaculture relevant traits, we can genotype breed desirable traits into farmed We generated ATAC-seq gene expression data from O. niloticus gill tissues, through integration SNPs 27 species, identified 1168 highly expressed genes (4% all genes) with accessible...