A5059392496- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Herpesvirus Infections and Treatments
- Aquaculture disease management and microbiota
- Gut microbiota and health
- Parasitic Infections and Diagnostics
- Animal Nutrition and Physiology
- Parasite Biology and Host Interactions
- Probiotics and Fermented Foods
- Plant Virus Research Studies
- Geotechnical Engineering and Analysis
- Dam Engineering and Safety
- Grouting, Rheology, and Soil Mechanics
- Salmonella and Campylobacter epidemiology
- Helminth infection and control
- Extracellular vesicles in disease
- interferon and immune responses
- Biosensors and Analytical Detection
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Geotechnical Engineering and Underground Structures
- Heat shock proteins research
- Coccidia and coccidiosis research
Guangxi Veterinary Research Institute
2019-2024
Ministry of Agriculture and Rural Affairs
2023-2024
Guizhou Electric Power Design and Research Institute
2023-2024
PowerChina (China)
2014-2024
Guangxi Center for Disease Prevention and Control
2023
Xiamen University
2023
Tongji University
2023
Washington State University
2023
Guangzhou Medical University
2018-2020
Second Affiliated Hospital of Guangzhou Medical University
2019-2020
Poly (ADP-ribose) polymerase (PARP) plays a significant role in DNA repair responses; therefore, this enzyme is targeted by PARP inhibitors cancer therapy. Here we have developed number of fused tetra- or pentacyclic dihydrodiazepinoindolone derivatives with excellent enzymatic and cellular PARylation inhibition activities. These efforts led to the identification pamiparib (BGB-290, 139), which displays PARP-1 PARP-2 IC50 1.3 0.9 nM, respectively. In assay, compound inhibits activity = 0.2...
Lactobacillus plantarum has recently been found to be a natural source feed additive bacteria with great advantages in food safety and animal welfare. Discovering novel strains commercial application potentiation could benefit the local poultry industry, particular support Chinese farmers. In this study, we tested isolated strain of GX17 as on growth performance intestinal barrier functions 1-day-old yellow-feather chicks. As good other probiotics, feeding showed significant improvements...
Escherichia coli (E. coli) is a common pathogen that often causes diarrhea in piglets. Since bacteria are becoming more and resistant to antibiotics, phages have become promising alternative therapy. However, the therapy of oral phage fails achieve desired effect. A novel named A221 was isolated by using E. GXXW-1103 as host strain, characterized electron microscopy, genomic sequencing analyzed measuring lysis ability vitro.Phage identified member Ackermannviridae, Aglimvirinae, Agtrevirus...
Extracellular vesicles (EVs) released by helminths play an important role in parasite-host communication. However, little is known about the characteristics and contents of EVs Fasciola gigantica, a parasitic flatworm that causes tropical fascioliasis. A better understanding F. gigantica will help elucidate mechanism gigantica-host interaction facilitate search for new vaccine candidates control treatment fascioliasis.Two different populations (15k 100k EVs) were purified from adult culture...
Viperin is an interferon-inducible protein that responsible for a variety of antiviral responses to different viruses. Our previous study has shown the ribonuclease UL41 herpes simplex virus 1 (HSV-1) can degrade mRNA viperin promote HSV-1 replication. However, it not clear whether other encoded proteins regulate function viperin. Here, one novel associated protein, glycoprotein D (gD), was identified. To verify interaction between gD and viperin, expression plasmids were firstly...
To study the influence of shield excavation on surrounding soil, and to control subway tunnel surface settlement deformation buildings, it is necessary additional stress soil caused by law settlement. Combined with a construction project tunnel, this article uses Mindlin solution in elastic mechanics obtain three-dimensional calculation formulas for thrust cutter head displacement field friction force between shell contact interface soil. The Loganathan ground movement pattern used determine...
The safety and stability of tunnel construction are paramount concerns in engineering. Traditionally, the assessment involves evaluating whether displacement deformation velocities exceed acceptable thresholds, using specifications analogical reasoning. However, standardized cautionary indicators often lack adaptability can result unnecessary reinforcement increase economic costs. This study presents a method for determining limit based on states. Based finite difference method, which...
strains are potentially rich sources of probiotics that could help avoid infections. In order to evaluate their efficacy in bolstering resistance
The interferon (IFN) system is an extremely powerful antiviral response in animal cells. subsequent effects caused by porcine astrovirus type 1 (PAstV1) IFN activation are important for the host's to viral infections. Here, we show that this virus, which causes mild diarrhea, growth retardation, and damage of villi small intestinal mucosa piglets, induces upon infection PK-15 Although IFN-β mRNA was detected within infected cells, usually occurs during middle stages infection, after genome...
Typhimurium (
As an indispensable structure protein, the herpes simplex virus 1 (HSV-1) UL6 has been described to exert numerous roles in viral proliferation.However, its exact subcellular localization and transport mechanism is not well known.In present study, by utilizing confocal fluorescent microscopy, was shown mainly locate nucleus enhanced yellow protein or Flag tag fused expression plasmidtransfected cells HSV-1-infected cells, whereas predicted nuclear signal nonfunctional.In addition, exploiting...
Abstract Background Mycoplasma ovipneumoniae is a critical pathogen that causes respiratory diseases threaten Caprini health and cause economic damage. A genome-wide study of M. will help understand the pathogenic characteristics this microorganism. Results Toxicological pathology whole-genome sequencing nine strains isolated from goats were performed using an epidemiological survey. These exhibited anterior ventral lung consolidation, typical bronchopneumonia in goats. Average nucleotide...
Abstract Salmonellosis is a disease caused by non-typhoid Salmonella, and although some lactic acid bacteria strains have been shown previously to relieve symptoms, little has studied about the preventive mechanism of Lentilactobacillus buchneri ( L. ) against Salmonella infection in vivo. Therefore, was fed C57BL/6 mice for 10 days build protective system study its prevention possible mechanisms. The results showed that GX0328-6 alleviated symptoms typhimurium among mice, including low...
Bovine papillomaviruses (BPV) are small circular DNA viruses which can be widely spread in herd, inducing cattle tumours, therefore, leading economic losses dairy and beef production industries. BPV-leads symptoms include cutaneous papillomas, fibropapillomas, urinary bladder oesophageal carcinoma. As one of the most important producers world, China has not provided systematic research to prevent harm BPV, particularly papillomavirus molecular characterization presents among Chinese native...
In recent years, hunniviruses have been reported in a variety of animal species from many countries. Here, hunnivirus was detected fecal samples water buffaloes and named as BufHuV-GX-2106. The were inoculated into cultures MDBK cells supplemented with TPCK trypsin the BufHuV-GX-2106 strain stably passaged replicated. Electron microscopic analysis showed virus particles spherical 20~30 nm diameter. complete genome plaque purified sample determined analyzed. Genomic revealed that whole...
Epstein-Barr virus (EBV) is a universal herpes which can cause life-long and largely asymptomatic infection in the human population. However, exact pathogenesis of EBV not well known.
Escherichia coli (O78) is an avian pathogenic (APEC). It can cause perihepatitis, pericarditis, septicemia and even systemic infections in the poultry industry. With incidence of antibiotic resistance reaching a crisis point, it important to find alternative treatments for multidrug-resistant infections. The use phages control pathogens promising therapeutic option replacement. In this study, we isolated lytic phage called vB_EcoS_GN06 from sewage. lysed APEC GXEC-N22. Transmission electron...
Background: The UL31 protein of herpes simplex virus 1 (HSV-1) plays an important role in the HSV-1 replication, however, its pinpoint functions life cycle have yet to be adequately elucidated. Objectives: An antiserum specific for detecting was prepared as foundation future research on course infection. Materials and Methods: Recombinant expressed Escherichia coli, which then purified employed raise level mice. Subsequently, western blot immunofluorescence assay (IFA) were utilized detect...
Bovine coronavirus (BCoV) is a notable pathogen affecting newly born calves and adult cattle, increasing mortality rates among reducing productivity in meat dairy industries, thereby causing substantial economic losses. Current primary laboratory methods for detecting BCoV include RT-PCR assay, real-time ELISA. However, these are time-consuming, require specialized technicians, necessitate environment. Consequently, there an urgent need rapid, sensitive, easy to use diagnostic method detect...
Abstract Nanobodies, primarily derived from camelid animals, have gained prominence in antibody-drug development due to their small molecular weight and ease of modification. However, the high cost associated with animals for nanobody discovery led us develop a strain heavy-chain mice known as KY-HC-mouse. This genetically modified mouse expresses IgG without CH1 domain, resulting production nanobodies format. Utilizing hybridoma techniques, we developed variety against multiple targets,...