A5028724322- Cancer Genomics and Diagnostics
- Blood transfusion and management
- Advanced Proteomics Techniques and Applications
- Blood groups and transfusion
- Trypanosoma species research and implications
- Blood donation and transfusion practices
- Molecular Biology Techniques and Applications
- Health, Environment, Cognitive Aging
- RNA Research and Splicing
- Inflammatory Myopathies and Dermatomyositis
- Immune Cell Function and Interaction
- Celiac Disease Research and Management
- Epigenetics and DNA Methylation
- Research on Leishmaniasis Studies
- RNA and protein synthesis mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiovascular Disease and Adiposity
- Cardiovascular Issues in Pregnancy
- Bioinformatics and Genomic Networks
- Genetic factors in colorectal cancer
- Hepatitis C virus research
- Cancer-related molecular mechanisms research
- Pancreatic and Hepatic Oncology Research
- RNA modifications and cancer
- Immunodeficiency and Autoimmune Disorders
National Institutes of Health
2005-2024
National Cancer Institute
2014-2024
Westat (United States)
2009-2015
Lambda Instruments (United States)
2009-2013
Blood Systems Research Institute
2009-2013
Universidade Federal de Minas Gerais
2013
Universidade de São Paulo
2013
Mayo Clinic
2013
Syracuse University
2011
University of California, San Francisco
2009-2011
The benefits of endoscopic testing for colorectal-cancer screening are uncertain. We evaluated the effect with flexible sigmoidoscopy on incidence and mortality.
BACKGROUND: Antibodies to human leukocyte antigens (HLA) in donated blood have been implicated as a cause of transfusion-related acute lung injury (TRALI). A potential measure reduce the risk TRALI includes screening plateletpheresis donors for HLA antibodies. The prevalence antibodies and their relationship previous transfusion or pregnancy was determined. STUDY DESIGN AND METHODS: total 8171 volunteer were prospectively recruited by six US centers from December 2006 May 2007. Donors...
We describe the clinical features of 28 patients with juvenile dermatomyositis (JDM) and 1 patient adult-onset (DM), all whom developed lipodystrophy (LD) that could be categorized into 3 phenotypes, generalized, partial, or focal, based on pattern fat loss distribution. LD onset was often delayed, beginning a median 4.6 years after diagnosis DM. Calcinosis, muscle atrophy, joint contractures, facial rash were DM disease found to associated LD. Panniculitis focal lipoatrophy while anti-p155...
Very few studies have measured disease penetrance and prognostic factors of Chagas cardiomyopathy among asymptomatic Trypanosoma cruzi-infected persons.We performed a retrospective cohort study initially healthy blood donors with an index T cruzi-seropositive donation age-, sex-, period-matched seronegatives in 1996 to 2002 the Brazilian cities São Paulo Montes Claros. In 2008 2010, all subjects underwent medical history, physical examination, ECGs, echocardiograms. ECG echocardiogram...
Next Generation Sequencing (NGS) technologies are used to detect somatic mutations in tumors and study germ line variation. Most NGS studies use DNA isolated from whole blood or fresh frozen tissue. However, formalin-fixed paraffin-embedded (FFPE) tissues one of the most widely available clinical specimens. Their potential utility as a source for would greatly enhance population-based cancer studies. While preliminary suggest FFPE tissue may be NGS, feasibility using archived specimens...
As COVID-19 continues, an increasing number of patients develop long COVID symptoms varying in severity that last for weeks, months, or longer. Symptoms commonly include lingering loss smell and taste, hearing loss, extreme fatigue, "brain fog." Still, persistent cardiovascular respiratory problems, muscle weakness, neurologic issues have also been documented. A major problem is the lack clear guidelines diagnosing COVID. Although some studies suggest due to prolonged inflammation after...
The idiopathic inflammatory myopathies (IIM) are systemic connective tissue diseases defined by chronic muscle inflammation and weakness associated with autoimmunity.We have performed low to high resolution molecular typing assess the genetic variability of major histocompatibility complex loci (HLA-A, -B, -Cw, -DRB1, -DQA1) in a large population European American patients IIM (n = 571) representing myositis autoantibody groups.We established that alleles 8.1 ancestral haplotype (8.1 AH)...
The idiopathic inflammatory myopathies (IIM) are systemic connective tissue diseases in which autoimmune pathology is suspected to promote chronic muscle inflammation and weakness. We have performed low high resolution genotyping characterize the allelic profiles of HLA-A, -B, -Cw, -DRB1, -DQA1 loci a large population North American Caucasian patients with IIM representing major clinicopathologic groups (n = 571). confirmed that alleles 8.1 ancestral haplotype were important risk markers for...
Background Blood donor screening leads to large numbers of new diagnoses Trypanosoma cruzi infection, with most donors in the asymptomatic chronic indeterminate form. Information on electrocardiogram (ECG) findings infected blood is lacking and may help counseling recognizing those more severe disease. Objectives To assess frequency ECG abnormalities T.cruzi seropositive relative seronegative donors, recognize associated left ventricular dysfunction. Methods The study retrospectively...
(See the editorial commentary by Katz, on pages 867–9 and see article Stramer et al, 886–94.) Background. Genetic variations of human immunodeficiency virus (HIV), hepatitis C (HCV), B (HBV) can affect diagnostic assays therapeutic interventions. Recent changes in prevalence subtypes/genotypes drug/immune-escape variants were characterized comparing recently infected vs more remotely blood donors. Methods. Infected donors identified among approximately 34 million US donations, 2006–2009;...
Abstract Objective To define the relative importance (RI) of class II major histocompatibility complex (MHC) alleles and peptide binding motifs as risk or protective factors for juvenile dermatomyositis (DM), to compare these with HLA associations in adult DM. Methods DRB1 DQA1 typing was performed 142 Caucasian patients DM, results were compared data from 193 DM 797 race‐matched controls. Random Forests classification multiple logistic regression used assess RI associations. Results The...
Mass spectrometry-based proteomics has become a powerful tool for the identification and quantification of proteins from wide variety biological specimens. To date, majority studies utilizing tissue samples have been carried out on prospectively collected fresh frozen or optimal cutting temperature (OCT) embedded However, such specimens are often difficult to obtain, in limited supply, clinical information outcomes patients inherently delayed as compared banked samples. Annotated formalin...
Formalin-fixed paraffin-embedded (FFPE) tissues are among the most widely available clinical specimens. Their potential utility as a source of RNA for transcriptome studies would greatly enhance population-based cancer studies. Although preliminary suggest FFPE tissue may be used sequencing, effect storage time on these specimens needs to determined. We conducted this study determine whether in archived high-grade ovarian serous adenocarcinomas from Surveillance, Epidemiology and End Results...
BACKGROUND: Transfusion‐related acute lung injury (TRALI) is the leading cause of transfusion‐related mortality. Blood centers are implementing TRALI risk reduction strategies based on screening apheresis donors for antibodies to human leukocyte antigens (HLA). STUDY DESIGN AND METHODS: HLA antibody was performed 7920 blood from Leukocyte Antibody Prevalence Study (LAPS) using Luminex‐based normalized background (NBG) cutoff ratios 10.8 (Class I) and 6.9 II). Single antigen bead (SAB) assay...
There are few surveillance studies analyzing genotypes or primary (transmitted) drug resistance in HIV-infected blood donors Brazil. The aim of this study was to characterize patterns HIV and among HIV-seropositive identified at 4 geographically dispersed centers Brazil.All who returned for counseling the REDS-II Hemocenters Brazil from January 2007 March 2011 were invited participate a case-control involving questionnaire on risk factors. Viral sequencing also offered positive cases assign...
BACKGROUND: We used a multicenter retrospective cohort study design to evaluate whether human leukocyte antigen (HLA) antibody donor screening would reduce the risk of transfusion‐related acute lung injury (TRALI) or possible TRALI. STUDY DESIGN AND METHODS: In Leukocyte Antibody Prevalence Study‐II (LAPS‐II), we evaluated pulmonary outcomes in recipients 2596 plasma‐rich blood components (transfusable plasma and plateletpheresis) sent participating hospitals; half were collected from...
BACKGROUND: The clinical significance of anti‐ Trypanosoma cruzi low‐level reactive samples is incompletely understood. Polymerase chain reaction (PCR)‐positive rates and antibody levels among seropositive blood donors in three countries are described. STUDY DESIGN AND METHODS: Follow‐up were collected from T. –seropositive 2008 through 2010 the United States (n = 195) Honduras 58). Also 143 Brazil 1996 to 2002, originally positive by serologic assays, available paired with contemporary...
Tristetraprolin (TTP), a member of the tandem CCCH zinc finger protein family, promotes deadenylation tumor necrosis factor-alpha and granulocyte-macrophage colony-stimulating factor mRNAs after binding to AU-rich elements (ARE) in their 3'-untranslated regions. The high affinity TTP-ARE occurs between domain preferred nonamer UUAUUUAUU. By mutating well defined core sequence 24 bases from ARE, we compared influence four possible nonameric TTP-binding sites wild-type ARE with that single...
BACKGROUND: Transfusion‐related acute lung injury (TRALI) has been associated with both human leukocyte antigen (HLA) and neutrophil (HNA) antibodies. HNA antibody frequency, specificity, demographic associations have not well defined in the blood donor population. STUDY DESIGN AND METHODS: A subset of 1171 donors (388 nontransfused males, 390 HLA antibody–negative females three or more pregnancies, 393 antibody–positive pregnancies) from a larger Leukocyte Antibody Prevalence Study was...