A5101700493- Chromosomal and Genetic Variations
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Parasitic Infections and Diagnostics
- CRISPR and Genetic Engineering
- Antibiotic Resistance in Bacteria
- Crystallization and Solubility Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- X-ray Diffraction in Crystallography
- Natural Compound Pharmacology Studies
- Genomics and Phylogenetic Studies
- Plant chemical constituents analysis
- Healthcare and Environmental Waste Management
- Ubiquitin and proteasome pathways
- Health, Environment, Cognitive Aging
- Cell Adhesion Molecules Research
- Metal-Catalyzed Oxygenation Mechanisms
- Gastrointestinal disorders and treatments
- Water Treatment and Disinfection
- Fish biology, ecology, and behavior
- Milk Quality and Mastitis in Dairy Cows
- Identification and Quantification in Food
- Livestock Management and Performance Improvement
- Genetics, Aging, and Longevity in Model Organisms
Indian Institute of Technology Roorkee
2016-2022
Johns Hopkins University
2011-2012
Jawaharlal Nehru University
2004-2012
University of Pennsylvania
2007-2010
University of Allahabad
1982
Human retrotransposons generate structural variation and genomic diversity through ongoing retrotransposition non-allelic homologous recombination.Cell culture assays have provided great insight into the impact of retrotransposons, in particular, LINE-1(L1) Alu elements; however, no such assay exists for youngest active human retrotransposon, SINE-VNTR-Alu (SVA).Here we report development an SVA cell assay.We marked several SVAs with either neomycin or EGFP indicator cassettes.Engineered...
Despite the immense significance retrotransposons have had for genome evolution much about their biology is unknown, including processes of forming ribonucleoprotein (RNP) particles and transporting them cell. Suppression retrotransposon expression, together with presence sequence within numerous mRNAs, makes tracking endogenous L1 RNP in cells problematic. We overcame these difficulties by assaying living fixed tagged-RNPs generated from constructs expressing retrotransposition-competent...
Long INterspersed Elements (LINE-1s, L1s) are responsible for over one million retrotransposon insertions and 8000 processed pseudogenes (PPs) in the human genome. An active L1 encodes two proteins (ORF1p ORF2p) that bind with RNA form L1-ribonucleoprotein particles (RNPs). Although it is believed RNA-binding property of ORF1p critical to recruit other mobile RNAs RNP, identity recruited largely unknown. Here, we used crosslinking immunoprecipitation followed by deep sequencing identify...
Most new genes arise by duplication of existing gene structures, after which relaxed selection on the copy frequently leads to mutational inactivation duplicate; only rarely will a with modified function emerge. Here we describe unique mechanism creation, whereby combinations functional domains are assembled at RNA level from distinct genes, and resulting chimera is then reverse transcribed integrated into genome L1 retrotransposon. We characterized novel gene, termed PIP5K1A PSMD4-like (...
RNA-based duplication mediated by reverse transcriptase (RT), a process termed retrotransposition, is ongoing in humans and source of significant inter- perhaps intraindividual genomic variation. The long interspersed element 1 (LINE-1 or L1) ORF2 protein the for RT activity required mobilization its own RNA cis other RNAs, such as SINE/variable-number tandem-repeat (VNTR)/Alu (SVA) elements, trans. SVA elements are ∼2-kb hominid-specific noncoding RNAs that have resulted single-gene disease...
Recent reports indicate that retrotransposons – a type of mobile DNA can contribute to neuronal genetic diversity in mammals. Retrotransposons are elements mobilize via an RNA intermediate by "copy-and-paste" mechanism termed retrotransposition. Long Interspersed Element-1 (LINE-1 or L1) is the only active autonomous retrotransposon humans and its activity responsible for ~ 30% genomic mass. Historically, L1 retrotransposition was thought be restricted germline; however, new data s somatic...
ABSTRACT The non-long-terminal-repeat (non-LTR) retrotransposons (also called long interspersed repetitive elements [LINEs]) are among the oldest retroelements. Here we describe properties of such an element from a primitive protozoan parasite, Entamoeba histolytica , that infects human gut. This 4.8-kb element, EhLINE1, is present in about 140 copies dispersed throughout genome. belongs to R4 clade non-LTR elements. It has centrally located reverse transcriptase domain and restriction...
The genome of the human pathogen Entamoeba histolytica contains non-long terminal repeat (LTR) retrotransposons, EhLINEs and EhSINEs, which lack targeted insertion. We investigated importance local DNA structure, sequence preference element-encoded endonuclease (EN) in selecting target sites for retrotransposon Pre-insertion loci were tested computationally to detect unique features based on thermodynamic considerations protein interaction measures. Target could readily be distinguished from...
The azo dye methyl red, which has carboxylato and functional groups, was used as a ligand for the synthesis of cyclometalated Ru III complex [Ru(L 1 )(PPh 3 ) 2 Cl] ( , L H = 2‐{[4‐(dimethylamino)phenyl]diazenyl}benzoic acid) through C–H bond activation. Complex treated with nitric oxide to afford organometallic ruthenium nitrosyl H)(PPh (NO)Cl][ClO 4 ] 1a 2‐{[4‐(dimethylamino)‐3‐nitrophenyl]diazenyl}benzoic acid). molecular structures 1· CH OH 1a· were determined by X‐ray crystallography....
The genome of the human pathogen Entamoeba histolytica, a primitive protist, contains non-long terminal repeat retrotransposable elements called EhLINEs. These encode reverse transcriptase and endonuclease required for retrotransposition. shows sequence similarity with bacterial restriction endonucleases. Here we report salient enzymatic features one such endonuclease. kinetics an EhLINE1-encoded catalyzed reaction, determined under steady-state single-turnover conditions, revealed...
There are mixed opinions on whether municipal wastewater treatment plants (MWTP) act as a significant hub for the selective growth and proliferation of antibiotic-resistant bacteria. In this study, investigations were carried out occurrence fate fluoroquinolone (FQ) antibiotics bacteria at different units within an activated sludge-based MWTP in India. The results indicated that raw inlet had concentrations ciprofloxacin, norfloxacin, ofloxacin range 6–17 μg/L, which was significantly higher...
Abstract Background Oral Squamous Cell Carcinoma (OSCC) results from a series of genetic alteration in squamous cells. This particular type cancer considers one the most aggressive malignancies to control because its frequent local invasions regional lymph node. Although several biomarkers have been reported, key marker used predict behavior disease is largely unknown. Here we report Long INterpersed Element-1 (LINE1 or L1) retrotransposon activity post-operative oral samples. L1 only active...
Offals are entrails and internal organs of animals used as food. Its consumption has a tremendous regional impact that ranges from local to global where range factors like social, economic cultural determine the consumer behaviour towards offal meat consumption. The present study was purposively carried out in Srinagar city Kashmir region order understand behavior offals among consumers this district. major findings reveal majority households were Muslims, living nuclear...