A5038390829- Amoebic Infections and Treatments
- Parasitic Infections and Diagnostics
- Marine and Offshore Engineering Studies
- RNA modifications and cancer
- Adipose Tissue and Metabolism
- RNA and protein synthesis mechanisms
- Global Energy and Sustainability Research
- Enzyme-mediated dye degradation
- Muscle metabolism and nutrition
- Diagnosis and treatment of tuberculosis
- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Outsourcing and Supply Chain Management
- Offshore Engineering and Technologies
- Structural Health Monitoring Techniques
- Mosquito-borne diseases and control
- Biochemical and biochemical processes
- Viral Infections and Vectors
- Sirtuins and Resveratrol in Medicine
- Metabolism and Genetic Disorders
- Lignin and Wood Chemistry
- Biliary and Gastrointestinal Fistulas
- Innovative concrete reinforcement materials
- RNA Research and Splicing
- Digital Imaging for Blood Diseases
Case Western Reserve University
2022-2023
Nepal Medical College Teaching Hospital
2023
Cleveland Clinic Lerner College of Medicine
2020-2023
Jawaharlal Nehru University
2017-2022
Cleveland Clinic
2020-2022
University Teaching Hospital
2022
Madda Walabu University
2021
International Centre for Genetic Engineering and Biotechnology
2012-2016
Oil and Natural Gas Corporation (India)
2006
Abstract Background Chikungunya (CHIK) is currently endemic in South and Central India exist as co-infections with dengue Northern India. In 2010, New Delhi witnessed an outbreak of CHIK the months October-December. This was first incidence a dominant prompted us to characterize virus strains. We have also investigated evolution spread Findings Clinical samples were subjected RT-PCR detect viral RNA. The PCR amplified products sequenced resulting sequences genetically analyzed. Phylogenetic...
Abstract Background Lignin is the second most abundant natural polymer on earth. Industries using lignocellulosic biomass as feedstock generate a considerable amount of lignin byproduct with minimal usage. For sustainable biorefinery, must be utilized in improved ways. recalcitrant to degradation due complex and heterogeneous structure. The depolymerization its conversion into specific product stream are major challenges associated valorization. blend oligomeric, dimeric monomeric...
Abstract Perturbed metabolism of ammonia, an endogenous cytotoxin, causes mitochondrial dysfunction, reduced NAD + /NADH (redox) ratio, and postmitotic senescence. Sirtuins are ‐dependent deacetylases that delay In multiomics analyses, sirtuin pathways enriched during hyperammonemia. Consistently, Sirtuin3 (Sirt3) expression deacetylase activity were decreased, protein acetylation was increased in human murine skeletal muscle/myotubes. Global acetylomics subcellular fractions from myotubes...
Interactions between RNAs and RNA binding proteins (RBPs) regulate gene expression in eukaryotic cells. RNA-RBP affinities measured vitro reveal diverse specificities, yet approaches to directly compare specificities across RBPs are lacking. Here, we introduce two quantitative metrics: inherent specificity, which measures how selectively an RBP distinguishes its strongest motif from all possible motifs, mutational sensitivity, assesses tolerance single nucleotide variations within preferred...
Signaling and metabolic perturbations contribute to dysregulated skeletal muscle protein homeostasis secondary sarcopenia in response a number of cellular stressors including ethanol exposure. Using an innovative multiomics-based curating unbiased data, we identified molecular therapeutic targets experimentally validated restoration ethanol-fed mouse model liver disease.Studies were performed ethanol-treated differentiated C2C12 myotubes physiological relevance established alcohol-related...
Culture depicts the identity and creativity representing a specific society or region. It is essential for growth of humans has significant impact on how individuals interact with one another in daily life. When culture powerful, it can significantly improve community’s health general well-being. This article’s goals are to illustrate value creating sense community examine Various cultural aspects Bihar region which play important role bring people together happy events. research paper...
Nocturnal hypoxaemia, which is common in chronic obstructive pulmonary disease (COPD) patients, associated with skeletal muscle loss or sarcopenia, contributes to adverse clinical outcomes. In COPD, we have defined this as prolonged intermittent hypoxia (PIH) because the duration of occurs through sleep followed by normoxia during day, contrast recurrent brief hypoxic episodes apnoea (OSA). Adaptive cellular responses PIH are not known. Responses induced three cycles 8 h 16 were compared...
Ammonia is a cytotoxic metabolite with pleiotropic molecular and metabolic effects, including senescence induction. During dysregulated ammonia metabolism, which occurs in chronic diseases, skeletal muscle becomes major organ for nonhepatocyte uptake. Muscle disposal mitochondria via cataplerosis of critical intermediary α-ketoglutarate, senescence-ameliorating molecule. Untargeted mitochondrially targeted data were analyzed by multiomics approaches. These analyses validated experimentally...
Promoter motifs in Entamoeba histolytica were earlier analysed using microarray data with lower dynamic range of gene expression. Additionally, previous transcriptomic studies did not provide information on the nature highly transcribed genes, and downstream promoter important for To address these issues we generated RNA-Seq identified high low expressing especially respect to virulence potential. We sequences both upstream start site motifs. used classify genes according expression levels,...
Ammonia is a cytotoxic molecule generated during normal cellular functions. Dysregulated ammonia metabolism, which evident in many chronic diseases such as liver cirrhosis, heart failure, and obstructive pulmonary disease, initiates hyperammonemic stress response tissues including skeletal muscle myotubes. Perturbations levels of specific regulatory molecules have been reported, but the global responses to hyperammonemia are unclear. In this study, we used multiomics approach vertically...
Summary RNA binding proteins (RBPs) are essential for metabolism and profoundly impact health disease. The subcellular organization of RBP interaction networks with target RNAs remains largely unexplored. Here, we develop colocalization CLIP, a method that combines CrossLinking ImmunoPrecipitation (CLIP) proximity labeling, to explore in-depth the interactions well-studied RNA-binding protein HuR. Using this method, uncover HuR’s dynamic location-specific RNA, revealing alterations in...
Abstract Entamoeba histolytica is responsible for dysentery and extraintestinal disease in humans. To establish successful infection, it must generate adaptive response against stress due to host defense mechanisms. We have developed a robust proteomics workflow by combining miniaturized sample preparation, low flow‐rate chromatography, ultra‐high sensitivity mass spectrometry, achieving increased proteome coverage, further integrated RNA‐seq data decipher regulation at translational...
Transceptors, solute transporters that facilitate intracellular entry of molecules and also initiate signaling events, have been primarily studied in lower-order species. Ammonia, a cytotoxic endogenous metabolite, is converted to urea hepatocytes for urinary excretion mammals. During hyperammonemia, when hepatic metabolism impaired, nonureagenic ammonia disposal occurs skeletal muscle. Increased uptake muscle mediated by membrane-bound, 12 transmembrane domain transporter, Rhesus blood...