A5071229336- Cancer Genomics and Diagnostics
- Lung Cancer Treatments and Mutations
- Lung Cancer Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Genetic factors in colorectal cancer
- Cancer Immunotherapy and Biomarkers
- RNA modifications and cancer
- Head and Neck Cancer Studies
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Electron and X-Ray Spectroscopy Techniques
- CRISPR and Genetic Engineering
- Advancements in Photolithography Techniques
- Lung Cancer Research Studies
- BRCA gene mutations in cancer
- Integrated Circuits and Semiconductor Failure Analysis
- Pancreatic and Hepatic Oncology Research
- Boron Compounds in Chemistry
- Inertial Sensor and Navigation
- Neuroendocrine Tumor Research Advances
- Iron Metabolism and Disorders
- Hemoglobinopathies and Related Disorders
- Effects of Radiation Exposure
- Nonmelanoma Skin Cancer Studies
- Ultrasound and Hyperthermia Applications
University of Toledo
2016-2024
University of Toledo Medical Center
2020-2023
Abstract Background Oncopanel genomic testing, which identifies important somatic variants, is increasingly common in medical practice and especially clinical trials. Currently, there a paucity of reliable reference samples having suitably large number pre-identified variants for properly assessing oncopanel assay analytical quality performance. The FDA-led Sequencing Quality Control Phase 2 (SEQC2) consortium analyze ten diverse cancer cell lines individually their pool, termed Sample A, to...
Abstract Introduction Melanoma, a deadly form of skin cancer, has witnessed notable increase in incidence over the past decades. Despite advancements treatment, it remains significant cause cancer mortality. Understanding demographic trends and variations melanoma mortality is crucial for addressing disparities implementing effective interventions. Methods Using Centers Disease Control Wide Ranging Online Data Epidemiologic Research (CDC WONDER) database, we analyzed data United States from...
Abstract Background Targeted sequencing using oncopanels requires comprehensive assessments of accuracy and detection sensitivity to ensure analytical validity. By employing reference materials characterized by the U.S. Food Drug Administration-led SEquence Quality Control project phase2 (SEQC2) effort, we perform a cross-platform multi-lab evaluation eight Pan-Cancer panels assess best practices for oncopanel sequencing. Results All demonstrate high across targeted high-confidence coding...
The primary objective of the FDA-led Sequencing and Quality Control Phase 2 (SEQC2) project is to develop standard analysis protocols quality control metrics for use in DNA testing enhance scientific research precision medicine. This study reports a targeted next-generation sequencing (NGS) method that will enable more accurate detection actionable mutations circulating tumor (ctDNA) clinical specimens. To accomplish this, synthetic internal spike-in was designed each mutation target,...
Abstract Background Clinical laboratories routinely use formalin-fixed paraffin-embedded (FFPE) tissue or cell block cytology samples in oncology panel sequencing to identify mutations that can predict patient response targeted therapy. To understand the technical error due FFPE processing, a robustly characterized diploid line was used create with four different pre-tissue processing formalin fixation times. A total of 96 sections were then distributed for analysis by oncopanels, and...
Standardized Nucleic Acid Quantification for SEQuencing (SNAQ-SEQ) is a novel method that utilizes synthetic DNA internal standards spiked into each sample prior to next generation sequencing (NGS) library preparation. This was applied analysis of normal appearing airway epithelial cells (AEC) obtained by bronchoscopy in an effort define somatic mutation field effect associated with lung cancer risk. There need biomarkers reliably detect those at highest risk, thereby enabling more effective...
Evidence of a systemic response related to localized radiation therapy (RT) in cancer management is rare. However, enhancing the immune via immunotherapy followed by RT has shown evidence tumor shrinkage non-irradiated metastatic disease thereby inducing an "abscopal effect." Combined induction cGAS-STING pathway and activation IFN-gamma signaling cascade within activated environment promotes neoantigen presentation expansion cytotoxic effector cells enabling enhancement response. A proposed...
There is a need for biomarkers that improve accuracy compared with current demographic risk indices to detect individuals at the highest lung cancer risk. Improved determination will enable more effective screening and better stratification of nodules into high or low-risk category. We previously reported discovery biomarker characterized by increased prevalence TP53 somatic mutations in airway epithelial cells (AEC). Here we present results from validation study an independent retrospective...
Acknowledging the correlation of response to therapy based on “targeting target” concept, FDA demonstrated confidence in precision approaches by approving Foundation One® CDx test late 2017 as an indicated diagnostic for cancer patients. More than 100 therapies involving both solid and liquid malignancy have since been approved a variety types related correlated molecular target. We provide clinical justification consideration guided matched target, specifically focusing PI3K/mTOR/AKT, BRCA,...
Abstract Clinical laboratories routinely use formalin-fixed paraffin-embedded (FFPE) tissue or cell block cytology samples in oncology panel sequencing to identify mutations that can predict patient response targeted therapy. To understand the technical error due FFPE processing, a robustly characterized normal line was used create with four different pre-tissue processing formalin fixation times. A total of 96 sections were then distributed for analysis by oncopanels, and variants resulting...
SUMMARY The primary objective of the FDA-led Sequencing and Quality Control Phase 2 (SEQC2) project is to develop standard analysis protocols quality control metrics for use in DNA testing enhance scientific research precision medicine. This study reports a targeted next generation sequencing (NGS) method that enables more accurate detection actionable mutations circulating tumor (ctDNA) clinical specimens. advancement was enabled by designing synthetic internal spike-in each mutation...
Abstract Background and Purpose: Reliable measurement of circulating free genomic DNA (cfDNA) methylation patterns promises a means for cancer early detection as well treatment response. Use Next Generation Sequencing (NGS) methods to measure the cfDNA methylome across large number potentially methylated CpG sites may enable earlier by overcoming stochastic sampling associated with small specimens obtainable blood draw. Wide-spread adoption these clinical diagnostic applications will be...
Abstract Background and Purpose: Early detection of lung cancer through annual screening with low-dose computed tomography (LDCT) scans enables potentially curative surgical or stereotactic body radiation therapy (SBRT) treatment thereby significantly reduces mortality. However, LDCT among those currently eligible based primarily on age smoking criteria yields a large number false-positive findings many cancers occur individuals who are ineligible. A biomarker that identifies risk...
Abstract Introduction Pancreatic cancer is a significant public health concern and leading cause of cancer-related deaths worldwide. This study aimed to investigate pancreatic mortality trends disparities in the United States (US) from 1999 2020. Methods Data were obtained Centers for Disease Control (CDC) Wide-Ranging Online Epidemiologic Research database. Mortality rates age-adjusted standardized year 2000 US population. Joinpoint regression was used analyze temporal (AAMRs) by...
Abstract Background: Genome-wide association studies (GWAS) and candidate gene suggest that telomere maintenance genes participate in etiology of lung cancer, idiopathic pulmonary fibrosis, COPD. Telomeres are nucleoprotein structures located at the end chromosomes maintained by telomerase encoded hTERT. Telomerase is active cancers, has ability to aid rapid cell division, dysfunctional chronic conditions induced inflammation as such diabetes, renal failure, hTERT a known downstream target...
Abstract Background. Based on increasing evidence from this laboratory and genome wide association studies (GWAS), single nucleotide polymorphisms (SNPs) responsible for inter-individual variation in normal bronchial epithelial cell (NBEC) cis-regulation of antioxidant, DNA repair, cycle control genes are key determinants lung cancer risk. Thus, there is a need NBEC culture methods that enable extended population doublings without genetic alteration to experimental investigation putative...
Abstract Background There is a need for biomarkers that improve accuracy compared with current demographic risk indices to detect individuals at the highest lung cancer risk. Improved determination will enable more effective screening and better stratification of nodules into high or low-risk category. We previously reported discovery biomarker characterized by increased prevalence TP53 somatic mutations in airway epithelial cells (AEC). Here we present results from validation study an...