A5035032193- Cancer Genomics and Diagnostics
- Carcinogens and Genotoxicity Assessment
- DNA Repair Mechanisms
- Genetic factors in colorectal cancer
- Molecular Biology Techniques and Applications
- Colorectal Cancer Treatments and Studies
- Lung Cancer Treatments and Mutations
- Meteorological Phenomena and Simulations
- Cancer-related Molecular Pathways
- Air Quality and Health Impacts
- CRISPR and Genetic Engineering
- Geophysics and Gravity Measurements
- Plant Genetic and Mutation Studies
- Gynecological conditions and treatments
- Endometriosis Research and Treatment
- DNA and Nucleic Acid Chemistry
- Estrogen and related hormone effects
- Uterine Myomas and Treatments
- Endometrial and Cervical Cancer Treatments
- Atmospheric and Environmental Gas Dynamics
- Cancer Cells and Metastasis
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- Pancreatic and Hepatic Oncology Research
- BRCA gene mutations in cancer
National Center for Toxicological Research
2014-2024
United States Food and Drug Administration
2014-2024
Barnet Hospital
2015
University of California, Berkeley
1982-2014
University of South Florida
1996-2014
Institute for Transfusion Medicine
2010
Johns Hopkins Hospital
2010
Center for Innovation
2010
University of Arkansas for Medical Sciences
2007
Central Arkansas Veterans Healthcare System
2007
From Cold Spring
BACKGROUND: Red blood cell (RBC) product wastage in hospitals is reported to range from 0.1% 6.7%. Wastage at our institution averaged 4.4% of 63,000 issued RBC products. Data indicated that approximately 87% wasted units were either individual out bank for more than 30 minutes (dispensed but not administered) or packed transport containers had temperature indicators affixed each unit. We hypothesized Lean Sigma methodology could be used reduce by 50%. STUDY DESIGN AND METHODS: An...
The hypothesis that most cancers are of monoclonal origin is often accepted as a fact in the scientific community. This dogma arose decades ago, primarily from study hematopoietic malignancies and sarcomas, which originate tumors. possible clonal malignant mesothelioma (MM) has not been investigated. Asbestos inhalation induces chronic inflammatory response at sites fiber deposition may lead to transformation after 30-50 years latency. As many mesothelial cells simultaneously exposed...
Abstract Background Oncopanel genomic testing, which identifies important somatic variants, is increasingly common in medical practice and especially clinical trials. Currently, there a paucity of reliable reference samples having suitably large number pre-identified variants for properly assessing oncopanel assay analytical quality performance. The FDA-led Sequencing Quality Control Phase 2 (SEQC2) consortium analyze ten diverse cancer cell lines individually their pool, termed Sample A, to...
Examination of the genomes 10 white-pock variants cowpox virus strain Brighton red (CPV-BR) revealed that 9 them had lost 32 to 38 kilobase pairs (kbp) from their right-hand ends and deleted sequences been replaced by inverted copies regions 21 50 kbp long left-hand end genome. These thus possess terminal repeats (ITRs) long; all are longer than ITRs CPV-BR (10 kbp). The 10th variant is a simple deletion mutant has between 12 limits inner observed deletions (between genome) appear be defined...
Chronic exposure to high concentrations of hexavalent chromium [Cr(VI)] as sodium dichromate dihydrate (SDD) in drinking water induces duodenal tumors mice, but the mode action (MOA) for these has been a subject scientific debate. To evaluate tumor-site-specific genotoxicity and cytotoxicity SDD mouse small intestine, tissue pathology cytogenetic damage were evaluated crypt villus enterocytes from B6C3F1 mice exposed 0.3–520 mg/L 7 90 days. Allele-competitive blocker PCR (ACB-PCR) was used...
Mutant cancer subpopulations have the potential to derail durable patient responses molecularly targeted therapeutics, yet prevalence and size of such are largely unexplored. We employed sensitive quantitative Allele-specific Competitive Blocker PCR approach characterize mutant in ductal carcinomas (DCs), examining five specific hotspot point mutations (PIK3CA H1047R, KRAS G12D, G12V, HRAS BRAF V600E). As an aid interpretation DC results, were also quantified normal breast tissue. Overall,...
Abstract Background Targeted sequencing using oncopanels requires comprehensive assessments of accuracy and detection sensitivity to ensure analytical validity. By employing reference materials characterized by the U.S. Food Drug Administration-led SEquence Quality Control project phase2 (SEQC2) effort, we perform a cross-platform multi-lab evaluation eight Pan-Cancer panels assess best practices for oncopanel sequencing. Results All demonstrate high across targeted high-confidence coding...
K-RAS mutation is being developed as a cancer biomarker and tumor used to predict therapeutic response. Yet, levels of in normal pathological tissue samples have not been determined rigorously, nor inter-individual variation these characterized. Therefore, codon 12 GAT GTT mutant fractions were measured colonic mucosa individuals without colon cancer, tumor-distal mucosa, tumor-proximal tumor-adjacent tissues, adenomas, carcinomas. The results indicate present at measurable appearing mucosa....
K-RAS mutation is being developed as a cancer biomarker and tumor used to predict therapeutic response. Yet, levels of in normal pathological tissue samples have not been determined rigorously, nor inter-individual variation these characterized. Therefore, codon 12 GAT GTT mutant fractions were measured colonic mucosa individuals without colon cancer, tumor-distal mucosa, tumor-proximal tumor-adjacent tissues, adenomas, carcinomas. The results indicate present at measurable appearing mucosa....
The potential use of K-RAS mutation as a cancer screening biomarker has been investigated for many years. Numerous associations between and various cancers have established, but these not translated into effective, cost-efficient strategies. This lack progress may be due to the existence in nontumor tissues and/or using detection, rather than quantitation, endpoint risk categorization. appears useful prognostic colon cancer. Recent toward sensitive quantitative characterization successful...
Aristolochic acid (AA) is a strong cytotoxic nephrotoxin and carcinogen associated with the development of urothelial cancer in humans. AA induces forestomach, kidney tract tumours rats mice. This study was conducted to characterise AA's carcinogenic mechanism action compare allele-specific competitive blocker-polymerase chain reaction (ACB-PCR)-based early detection effect using two different tumour-relevant endpoints. H-Ras codon 61 CAA→CTA mutation analysed because it found rodent...
Ethylene oxide (EO) is a genotoxicant and mouse lung carcinogen, but whether EO carcinogenic through mutagenic mode of action remains unclear. To investigate this question, 8-week-old male Big Blue B6C3F1 mice (10 mice/group) were exposed to by inhalation—6h/day, 5 days/week for 4 weeks (0, 10, 50, 100, or 200 ppm EO) 8 12 EO). Lung DNA samples analyzed levels 3 K-ras codon mutations (GGT→GAT, GGT→GTT, GGT→TGT) using ACB-PCR. No measureable level TGT mutation was detected (ie, all mutant...