First results of endoscopic applications optical coherence tomography for in vivo studies human mucosa respiratory, gastrointestinal, urinary and genital tracts are presented. A novel OCT (EOCT) system has been created that is based on the integration a sampling arm an all-optical-fiber interferometer into standard devices using their biopsy channel to transmit low-coherence radiation investigated tissue. We have studied mucous membranes esophagus, larynx, stomach, bladder, uterine cervix...

We use optical coherence tomography (OCT) to perform a comprehensive program of in vivo and vitro structural imaging hard soft tissues within the oral cavity. have imaged different types healthy mucosa as well normal abnormal tooth structure. OCT is able differentiate between various keratinized non-keratinized with high resolution. also provide detailed information on clinical abnormalities (caries non-caries lesions) teeth guidance dental restorative procedures. Our investigations...

Background/aims: Since the majority of skin diseases are known to be accompanied by structural alterations, research efforts focused on development various novel diagnostic techniques capable providing in vivo information structure. An essential parameter here is spatial resolution. In this paper we demonstrate capabilities optical coherence tomography (OCT) detecting specific features thin and thick skin. A particular focus made identification OCT patterns typical certain pathological...

We report results of application our endoscopic optical coherence tomography (EOCT) system in clinical experiments to image human internal organs. Based on the experience studying more than 100 patients, we make first general conclusions place and capabilities this method diagnosing mucous membranes. It is demonstrated that EOCT can serve for several purposes such as performing directed biopsy, monitoring functional states body, guiding surgical other treatments post-operative recovery...

Purpose: Growing evidence suggests that dendrite retraction or degeneration in a subpopulation of the retinal ganglion cells (RGCs) may precede detectable soma abnormalities and RGC death glaucoma. Visualization lamellar structure inner plexiform layer (IPL) could advance clinical management fundamental understanding We investigated whether visible-light optical coherence tomography (vis-OCT) detect difference IPL sublayer thicknesses between small cohorts healthy glaucomatous subjects....

Non-invasive depth-resolved measurement of hemoglobin oxygen saturation (SaO(2)) levels in discrete blood vessels may have implications for diagnosis and treatment various pathologies. We introduce a novel Dual-Wavelength Photothermal (DWP) Optical Coherence Tomography (OCT) non-invasive SaO(2) vessel phantom. DWP OCT is linearly correlated with blood-gas measurements. demonstrate 6.3% precision measured phantom using DWP-OCT 800 765 nm excitation wavelengths. Sources uncertainty are...

We longitudinally imaged both the superficial and deep cortical microvascular networks in brains of healthy mice a mouse model stroke vivo using visible-light optical coherence tomography (vis-OCT). surgically implanted microprism sealed by chronic cranial window. The enabled vis-OCT to image entire depth cortex. Following implantation, we for 28 days found that it took around 15 microvessels recover from implantation surgery. After recovered, introduced ischemic strokes transient middle...

Abstract Imaging complex, non-planar anatomies with optical coherence tomography (OCT) is limited by the field of view (FOV) in a single volumetric acquisition. Combining linear mechanical translation OCT extends FOV but suffers from inflexibility imaging anatomies. We report freeform robotic to fill this gap. To address challenges reconstruction associated movement accuracy being two orders magnitudes worse than resolution, we developed registration algorithm based on simultaneous...

We demonstrate the feasibility of swept-source visible-light optical coherence tomography (SS-vis-OCT). used a fanout periodically poled lithium niobate (PPLN) crystal for second-harmonic generation (SHG) to convert commercial near-infrared (NIR-SS) laser into SS laser. To enhance SHG efficiency, we amplified NIR-SS output with booster amplifiers (BOAs) and generated up 580 µW power. The SS-vis-OCT achieved maximum axial resolution 7.3 µm an imaging depth 5 mm in air, corresponding 5.4 3.7...

An experimental standard optical coherence tomography (OCT) setup that can be easily modified for cross-polarization OCT (CP OCT) operation has been developed to perform differential diagnosis of pathological tissues. The complementary use CP OCT, a technique provides map backscattering properties an object being studied by means low-coherence interferometry, and imaging improves the specificity diagnostics changes occurring in It is shown healthy, neoplastic scar tissues esophagus have...

Abstract When paramagnetic nanoparticles are adsorbed at the oil-water interface or dispersed in one of fluid phases reservoir rock pores, then exposed to an external magnetic field, resultant particle movements displace interface. Interfacial tension acts as a restoring force, leading interfacial fluctuation and pressure (sound) wave. Here we focus on motion. We apply theory ferrofluids case cylindrical pore. The predictions consistent with experiments aqueous suspension iron oxide nanorods...

We present a technique to reduce speckle in visible-light optical coherence tomography (vis-OCT) that preserves fine structural details and is robust against sample motion. Specifically, we locally modulate B-scans orthogonally their axis of acquisition. Such modulation enables acquisition uncorrelated patterns from similar anatomical locations, which can be averaged speckle. To verify the effectiveness reduction, performed in-vivo retinal imaging using modulated raster circular scans both...

Increases in speed and sensitivity enabled rapid clinical adoption of optical coherence tomography (OCT) ophthalmology. Recently, visible-light OCT (vis-OCT) achieved ultrahigh axial resolution, improved tissue contrast, provided new functional imaging capabilities, demonstrating the potential to improve care further. However, limited caused by high relative intensity noise (RIN) supercontinuum lasers impeded vis-OCT. To overcome these limitations, we developed balanced-detection vis-OCT...

Application of photothermal Optical Coherence Tomography (OCT) to detect macrophages in ex vivo rabbit arteries which have engulfed nanoclusters gold coated iron oxide (nanorose) is reported.Nanorose by associated with atherosclerotic lesions absorb incident laser (800nm) energy and cause optical pathlength (OP) variation measured using OCT.OP polydimethyl siloxane tissue phantoms containing varying concentrations nanorose match values predicted from nanoparticle material...

Magnetic resonance imaging (MRI) of the human retina faces two major challenges: eye movement and hardware limitation that could preclude retinal MRI with adequate spatiotemporal resolution. This study investigated eye-fixation stability high-resolution anatomic on a 3-Tesla (T) scanner. Comparison was made optical coherence tomography (OCT) same subjects.Eye-fixation protocols used in evaluated four normal volunteers using an tracker. High-resolution (100 × 200 2000 μm) protocol developed...

Abstract Background Retinal oxygen saturation (sO 2 ) provides essential information about the eye’s response to pathological changes that can result in vision loss. Visible-light optical coherence tomography (vis-OCT) is a noninvasive tool has potential measure retinal sO clinical setting. However, its reliability currently limited by unwanted signals referred as spectral contaminants (SCs), and comprehensive strategy isolate true oxygen-dependent from SCs vis-OCT lacking. Methods We...

Recently, our in vivo studies demonstrated a strong correlation between blood glucose concentration and the slope of optical coherence tomography (OCT) signal when probing beam was scanned over straight line. To improve sensitivity OCT for monitoring, two-dimensional (2D) lateral scanning proposed. Depth-dependent changes pig skin properties with variation were revealed due to significant suppression speckle noise motion artefacts 2D mode. The coefficient varied periodically range from −0.9...

The capabilities of visible-light optical coherence tomography (vis-OCT) in noninvasive anatomical and functional retinal imaging have been demonstrated by multiple groups both rodents healthy human subjects. Translating laboratory prototypes to an integrated clinical-environment-friendly system is required explore the full potential vis-OCT disease management.

Purpose: To develop a practical technique for visualizing and quantifying retinal ganglion cell (RGC) axon bundles in vivo. Methods: We applied visible-light optical coherence tomography (vis-OCT) to image the RGC bundles, referred as vis-OCT fibergraphy, of healthy wild-type C57BL/6 mice. After imaging, retinas were flat-mounted, immunostained with anti-beta-III tubulin (Tuj1) antibody axons, imaged confocal microscopy. quantitatively compared bundle networks by vivo ex microscopy using...

Abstract Melanoma accounts for 75% of all skin cancer deaths. Pulsed photothermal radiometry (PPTR), optical coherence tomography (OCT) and ultrasound (US) are non‐invasive imaging techniques that may be used to measure melanoma thickness, thus, determining surgical margins. We constructed a series PDMS tissue phantoms simulating melanomas different thicknesses. PPTR, OCT US measurements were recorded from results compared in terms axial range, resolution time. A Monte Carlo simulation...

Microvasculature hemoglobin oxygen saturation (SaO 2 ) is important in the progression of various pathologies.Non-invasive depthresolved measurement SaO levels tissue microvasculature has potential to provide early biomarkers and a better understanding pathophysiological processes allowing improved diagnostics prediction disease progression.We report proof-of-concept vivo depth-resolved selected 30 µm diameter arterioles murine brain using Dual-Wavelength Photothermal (DWP) Optical Coherence...

A combined application of optical methods [optical coherent tomography (OCT), cross-polarisation tomography, and fluorescence spectroscopy] is proposed for obtaining information on morphological biochemical changes occurring in tissues norm pathology. It shown that neoplastic scar esophagus can be distinguished using a combination polarisation standard OCT due to the difference between depolarising properties caused by structural collagenic fibres stroma. with spectroscopy use...

We proposed to use optical coherence tomography (OCT) for continuous noninvasive blood glucose monitoring, and recently we significantly improved the sensitivity of this technique. The accuracy OCT monitoring is limited by several factors, including variation tissue pressure exerted probe. studied influence high (>10 kPa) low (<1 levels on monitoring. showed that controlling external <1 kPa substantially reproducibility OCT-based