- Coffee research and impacts
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Yeasts and Rust Fungi Studies
- Plant tissue culture and regeneration
- Plant biochemistry and biosynthesis
- Forest Insect Ecology and Management
- Nematode management and characterization studies
- Phytoplasmas and Hemiptera pathogens
- Polyamine Metabolism and Applications
- Studies on Chitinases and Chitosanases
- Insect-Plant Interactions and Control
- Polysaccharides and Plant Cell Walls
- Photosynthetic Processes and Mechanisms
- Fermentation and Sensory Analysis
- Plant Pathogenic Bacteria Studies
- Nutrition, Genetics, and Disease
- Plant Pathogens and Resistance
- Transgenic Plants and Applications
- Plant Parasitism and Resistance
- Biochemical and biochemical processes
- Tree-ring climate responses
- Plant and Fungal Interactions Research
- Plant pathogens and resistance mechanisms
University of Lisbon
2016-2025
Universidade Nova de Lisboa
2023
University of Bristol
2023
Bridge University
2023
National Research Council
2023
Universidade do Porto
2023
Universidad de Granada
2023
Agriculture and Food
2022
Instituto de Investigação Científica Tropical
2002-2015
Indian Institute of Chemical Technology
2006
Considerable success has been obtained in the use of classical breeding to control economically important plant diseases, such as coffee leaf rust and berry disease (CBD). There is a strong consensus that growing genetically resistant varieties most appropriate cost effective means managing diseases one key components crop improvement. It also recognized better knowledge both, pathogens defence mechanisms will allow development novel approaches enhance durability resistance. After brief...
Coffee is one of the most consumed beverages in world. It also globally traded commodities. leaf rust (CLR), caused by biotrophic fungus Hemileia vastatrix, important disease affecting Arabica coffee growing worldwide, leading to significant yield losses if no control measures are applied. A deep understanding complex mechanisms involved coffee-H. vastatrix interactions, such as pathogen variability and governing plant resistance susceptibility, required breed efficiently for durable design...
Anthracnose is an important disease affecting mature olive fruits, causing significant yield losses, and poor fruit oil quality. In Portugal, high anthracnose incidence was recorded during 2003–2007 with 41% of 908 orchards surveyed displaying symptoms. another 14% the orchards, pathogen in symptomless plants. Disease severity on average 36%, frequently reaching 100%. endemic to neglected susceptible cultivars, but under favourable conditions it can also severely affect less cultivars....
Natural polyphenols are important dietary antioxidants that significantly benefit human health. Coffee and tea have been shown to largely contribute the intake of these in several populations. More recently, use coffee leaves produce has become a potential commercial target, therefore prompting studies on quantification leaves. In this study variety leaf species, at different development stages, were analyzed using ultra-high pressure liquid chromatography. The results demonstrate both...
SUMMARY The beverage cash crop coffee (Coffea arabica L.) is subject to severe losses caused by the rust fungus Hemileia vastatrix. In naturally resistant plants, a specific hypersensitive reaction (HR) may be elicited early stop fungal infection. To isolate host genes involved in HR, we undertook an expressed sequence tags (ESTs) analysis. Two cDNA libraries were constructed using suppression subtractive hybridization (SSH) and 527 non-redundant ESTs generated from 784 randomly picked...
A proteomic analysis of the apoplastic fluid (APF) coffee leaves was conducted to investigate cellular processes associated with incompatible (resistant) and compatible (susceptible) Coffea arabica-Hemileia vastatrix interactions, during 24-96 hai period. The APF proteins were extracted by leaf vacuum infiltration protein profiles obtained 2-DE. comparative gels revealed 210 polypeptide spots whose volume changed in abundance between samples (control, resistant susceptible) identified...
OPINION article Front. Plant Sci., 09 January 2017Sec. Pathogen Interactions Volume 7 - 2016 | https://doi.org/10.3389/fpls.2016.02051
BACKGROUND Plants have developed an efficient system of recognition that induces a complex network signalling molecules such as salicylic acid (SA), jasmonic (JA) and abscisic (ABA) in case pathogenic infection. The use specific sensitive methods is mandatory for the analysis compounds these samples. RESULTS In this study liquid chromatography/electrospray ionisation tandem mass spectrometry method was validated simultaneous quantification SA, JA ABA Coffea arabica (L.) leaves order to...
The analysis of complex biological systems keeps challenging researchers. main goal biology is to decipher interactions within cells, by integrating datasets from large scale analytical approaches including transcriptomics, proteomics and metabolomics more specialized ‘OMICS’ such as epigenomics lipidomics. Studying different cellular compartments allows a broader understanding cell dynamics. Plant apoplast, the compartment external plasma membrane wall, particularly demanding analyze....
Understanding the molecular mechanisms underlying coffee-pathogen interactions are of key importance to aid disease resistance breeding efforts. In this work expression genes involved in salicylic acid (SA), jasmonic (JA) and ethylene (ET) pathways were studied hypocotyls two coffee varieties challenged with hemibiotrophic fungus Colletotrichum kahawae, causal agent Coffee Berry Disease. Based on a cytological analysis, time-points infection process selected qPCR was used evaluate...
Two Coffea arabica - Hemileia vastatrix incompatible interactions (I1: coffee cv. Caturra rust race VI and I2: cv S4 Agaro II) a compatible interaction (coffee were compared in relation to the infection process chitinase activity. In two fungus ceased growth early stages, while no inhibition was observed. A high constitutive level of activity detected intercellular fluid healthy leaves. Upon infection, isoforms more abundant than interaction. Immunodetection showed that class I chitinases...
Epidemics of coffee leaf rust (CLR) leads to great yield losses and huge depreciation marketing values, if no control measures are applied. Societal expectations a more sustainable production increasingly imposing the replacement fungicide treatments by alternative solutions. A protection strategy is take advantage plant immune system eliciting constitutive defenses. Based on such concept, resistance inducers (PRIs) have been developed. The Greenforce CuCa formulation, similarly...
The hemibiotrophic fungus Colletotrichum kahawae is the causal agent of Coffee Berry Disease - CBD, an economically devastating disease restricted to Arabica coffee production in Africa. Understanding pathogen variability crucial for implementation control measures. In this study, six isoenzymatic systems (esterase, acid and alkaline phosphatase, malate dehydrogenase, peroxidase superoxide dismutase) were used assess genetic variation among 12 C. isolates (from different geographic origins...
Abiotic stresses significantly disrupt plant physiology at the molecular, biochemical, and morphological levels, often causing irreversible damage. To ensure sustainable coffee production, it is essential to understand how environmental stresses—such as drought, heat, excess light, salinity—affect growth, develop strategies mitigate their impact. Despite limited number of studies on this topic, compiling existing knowledge can provide valuable insights into plants respond such stresses....