A5042598252- Biochemical Acid Research Studies
- Biochemical and Molecular Research
- SARS-CoV-2 detection and testing
- Enzyme Structure and Function
- Genomics and Phylogenetic Studies
- Metabolism and Genetic Disorders
- SARS-CoV-2 and COVID-19 Research
- Biosensors and Analytical Detection
- Neonatal and Maternal Infections
- Carbohydrate Chemistry and Synthesis
- Streptococcal Infections and Treatments
- Pleural and Pulmonary Diseases
- Food Quality and Safety Studies
- COVID-19 Clinical Research Studies
- Glycosylation and Glycoproteins Research
- Diet, Metabolism, and Disease
- Pneumothorax, Barotrauma, Emphysema
- Molecular Biology Techniques and Applications
- Nosocomial Infections in ICU
- Amino Acid Enzymes and Metabolism
- Inflammasome and immune disorders
- Proteoglycans and glycosaminoglycans research
- Bipolar Disorder and Treatment
- Biliary and Gastrointestinal Fistulas
- Plant Pathogenic Bacteria Studies
University of Dundee
2018-2024
NHS Lothian
2021-2024
Edinburgh Royal Infirmary
2021-2023
Loyola University Chicago
2016-2018
Summary Biosynthesis of the nucleotide sugar precursor dTDP‐L‐rhamnose is critical for viability and virulence many human pathogenic bacteria, including Streptococcus pyogenes (Group A ; GAS), mutans Mycobacterium tuberculosis . Streptococcal pathogens require production structurally similar rhamnose polysaccharides in their cell wall. Via heterologous expression S. , we confirmed that GAS RmlB RmlC are biosynthesis through action as dTDP‐glucose‐4,6‐dehydratase...
To thrive in diverse environments, bacteria must shift their metabolic output response to nutrient bioavailability. In many bacterial species, such changes flux depend upon lipoic acid, a cofactor required for the activity of enzyme complexes involved glycolysis, citric acid cycle, glycine catabolism, and branched chain fatty biosynthesis. The requirement necessitates that synthesize and/or scavenge it from environmental sources. Although use is conserved phenomenon, mechanisms behind its...
Group A carbohydrate (GAC) is a bacterial peptidoglycan-anchored surface rhamnose polysaccharide (RhaPS) that essential for growth of Streptococcus pyogenes and contributes to its ability infect the human host. In this study, using molecular synthetic biology approaches, biochemistry, radiolabeling techniques, NMR MS analyses, we examined role GacB, encoded in S. GAC gene cluster, biosynthesis pathway. We demonstrate GacB first characterized α-d-GlcNAc-β-1,4-l-rhamnosyltransferase...
The Fructobacillus genus is a group of obligately fructophilic lactic acid bacteria (FLAB) that requires the use fructose or another electron acceptor for their growth. In this work, we performed comparative genomic analysis within by using 24 available genomes to evaluate and metabolic differences among these organisms. genome strains, which varies between 1.15- 1.75-Mbp, nineteen intact prophage regions, seven complete CRISPR-Cas type II systems were found. Phylogenetic analyses located...
Lipoic acid is a cofactor required for intermediary metabolism that either synthesized de novo or acquired from environmental sources. The bacterial pathogen Staphylococcus aureus encodes enzymes biosynthesis, but also two ligases, LplA1 and LplA2, are sufficient lipoic salvage during infection. S. H proteins, GcvH of the glycine cleavage system homologous GcvH-L encoded in an operon with LplA2. recognized conduit lipoyl transfer to α-ketoacid dehydrogenase E2 subunits, while function...
Abstract Group A Streptococcus (Strep A) cause a wide range of human-exclusive infections, annually killing more than 500,000 people. Antibiotic resistance incidence invasive Strep tripled in the past decade and emphasises need to develop universal vaccine. In this study, we developed recombinant rhamnose polysaccharides (RhaPS), validated vaccine candidate, presented on E. coli outer membrane vesicles (OMVs). We investigated OMV-RhaPS for their immunogenicity mouse rabbit models. Through...
In 2019, the European Union banned Triton X-100, a detergent widely used in laboratory diagnostics, including Viral PCR Sample Solution (VPSS), and urged manufacturers to find environmentally sustainable alternatives. Tergitol 15-S-9 (VPSS2) has been proposed as an alternative surfactant. This multicenter study evaluated effectiveness of VPSS2, Tergitol-based viral solution, replacement for VPSS. Our results show equivalent performance VPSS2 VPSS nucleic acid extraction stability over time...
SARS-CoV-2 antibody tests have been marketed to diagnose previous infection and as a test of immune status. There is lack evidence on the performance clinical utility these tests. We aimed carry out an evaluation 14 point care (POC) Serum from participants with RT-PCR (real-time polymerase chain reaction) confirmed pre-pandemic serum controls were used determine specificity sensitivity each POC device. Changes in increasing time determined cohort study participants. Corresponding...
Background Group A Streptococcus (GAS) are responsible for a wide range of human-exclusive infections, annually killing more than 500,000 people. Antibiotic resistance incidence invasive GAS tripled in the past decade and emphasises need to develop universal vaccine. We have produced, first time, recombinant polyrhamnose backbone (pRha), validated vaccine candidate. E. coli outer membrane vesicles (OMVs) carrying pRha were investigated their immunogenicity efficacy an animal model. Methods...
Summary Biosynthesis of the nucleotide sugar precursor dTDP-L-rhamnose is critical for viability and virulence many human pathogenic bacteria, including Streptococcus pyogenes (Group A ; GAS) mutans . Those pathogens require production structurally similar rhamnose polysaccharides in their cell wall. Via heterologous expression S. , we confirm that GAS RmlB RmlC are biosynthesis through action as dTDP-glucose-4,6-dehydratase dTDP-4-keto-6-deoxyglucose-3,5-epimerase enzymes, respectively....
Removing the swab after collection can speed up diagnosis and improve quality of laboratory procedures. This study investigates impact removal on SARS-CoV-2 detection in clinical specimens with a focus high Cycle Threshold (Ct) samples (Cts ≥32). The method assessed pairs mimicking nasopharyngeal tested them two real-time-PCR platforms; Applied Biosystems 7500 Abbott Alinity. Swab did not significantly affect rates Ct values <32, regardless PCR platform. However, reduced reproducibility...
Removing the swab after collection can speed up diagnosis and improve quality of laboratory procedures. This study investigates impact removal on SARS-CoV-2 detection in clinical specimens with a focus high Cycle Threshold (Ct) samples (Cts ≥32). The method assessed pairs mimicking combined throat nose swabs tested them two real-time-PCR platforms; Applied Biosystems 7500 Abbott Alinity. Swab did not significantly affect rates Ct values <32, regardless PCR platform. However, reduced...
Graphical Abstract Early removal of swabs can accelerate laboratory work and enhance quality by decreasing contamination errors in downstream procedures. This article aims to compare the traditional testing workflow a diagnostic (red), with manual swab aliquoting vs swab-out (blue).