A5037568691- Endoplasmic Reticulum Stress and Disease
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Bioinformatics and Genomic Networks
- Autophagy in Disease and Therapy
- Plant Virus Research Studies
- Transgenic Plants and Applications
- Genetic Mapping and Diversity in Plants and Animals
- Plant Pathogens and Fungal Diseases
- Plant Pathogens and Resistance
- Ubiquitin and proteasome pathways
- Spaceflight effects on biology
- Sugarcane Cultivation and Processing
- Genetics, Bioinformatics, and Biomedical Research
- Protein Degradation and Inhibitors
- Plant nutrient uptake and metabolism
- Plant Micronutrient Interactions and Effects
- Plant Pathogenic Bacteria Studies
- Crop Yield and Soil Fertility
- Cell Image Analysis Techniques
- Plant Gene Expression Analysis
- Radioactive contamination and transfer
- Heat shock proteins research
- Chromosomal and Genetic Variations
Michigan State University
2018-2025
Carnegie Institution for Science
2020-2025
Great Lakes Bioenergy Research Center
2020-2025
Carnegie Department of Plant Biology
2020
Michigan United
2018
The University of Texas at Austin
2015-2016
Seoul National University
2009
Heterosis has been widely used in agriculture, but the molecular mechanism for this remains largely elusive. In Arabidopsis hybrids and allopolyploids, increased photosynthetic metabolic activities are linked to altered expression of circadian clock regulators, including CIRCADIAN CLOCK ASSOCIATED1 (CCA1). It is unknown whether a similar mediates heterosis maize hybrids. Here we report that higher levels carbon fixation starch accumulation associated with temporal gene expression. Two CCA1...
Polyploidy provides evolutionary and morphological novelties in many plants some animals. However, the role of genome dosage composition gene expression changes remains poorly understood. Here, we generated a series resynthesized Arabidopsis tetraploids that contain 0–4 copies thaliana arenosa genomes investigated ploidy hybridity effects on expression. Allelic can be defined as dependent (expression levels correlate with dosages) or otherwise independent. show dosage-dependent genes...
Governed by the unfolded protein response (UPR), ability to counteract endoplasmic reticulum (ER) stress is critical for maintaining cellular homeostasis under adverse conditions. Unresolved ER leads cell death through mechanisms that are yet not completely known. To identify key UPR effectors involved in unresolved stress, we performed an ethyl methanesulfonate (EMS) suppressor screen on Arabidopsis bzip28/60 mutant, which impaired activating cytoprotective pathways. This identified MAP...
Abstract Adverse environmental and pathophysiological situations can overwhelm the biosynthetic capacity of endoplasmic reticulum (ER), igniting a potentially lethal condition known as ER stress. stress hampers growth triggers conserved cytoprotective signaling cascade, unfolded protein response (UPR) for homeostasis. As subsides, is resumed. Despite pivotal role UPR in restoration, underlying mechanisms resumption are yet unknown. To discover these, we undertook genomics approach model...
Abstract Bioenergy production often focuses on the aboveground feedstock for conversion to fuel and other materials. However, belowground component is crucial soil carbon sequestration, greenhouse gas fluxes, ecosystem function. Roots maximize marginal lands by acquiring resources mediating processes through interactions with microbial community. This world challenging observe quantify; however, there are unprecedented opportunities using current methodologies bring roots, microbes, into...
Genome-editing is being implemented in increasing number of plant species using engineered sequence specific nucleases (SSNs) such as Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated systems (CRISPR/Cas9), Transcription Activator Like Effector Nucleases (TALENs), and more recently CRISPR/Cas12a. As the tissue culture regeneration procedures to generate gene-edited events are time consuming, large-scale screening methodologies that rapidly facilitate validation...
Abstract Plant immunity depends on the secretion of antimicrobial proteins, which occurs through yet-largely unknown mechanisms. The trans -Golgi network (TGN), a hub for intracellular and extracellular trafficking pathways, cytoskeleton, is required protein secretion, are emerging as pathogen targets to dampen plant immunity. In this work, we demonstrate that tgnap1-2 , loss-of-function mutant Arabidopsis TGNap1, TGN-associated microtubule (MT)-binding protein, susceptible Pseudomonas...
Plants are primary producers of food and oxygen on Earth will likewise be indispensable to the establishment large-scale sustainable ecosystems human survival in space. To contribute understanding how plants respond spaceflight stress, we examined significance unfolded protein response (UPR), a conserved signaling cascade that responds number unfavorable environmental stresses, model plant Arabidopsis thaliana. do so, performed comparative transcriptome profiling wild type various...
Crop genetic engineering involves transformation in which transgenic plants are regenerated through tissue culture manipulations that can elicit somaclonal variation due to mutations, translocations, and/or epigenetic alterations. Here, we report on alterations the transcriptome a panel of potato engineered be herbicide resistant. Using an inbred diploid clone (DMRH S5 28–5), ten single-insert lines derived from independent Agrobacterium-mediated events were selected for resistance using...
Adverse environmental conditions reduce crop productivity and often increase the load of unfolded or misfolded proteins in endoplasmic reticulum (ER). This potentially lethal condition, known as ER stress, is buffered by protein response (UPR), a set signaling pathways designed to either recover functionality ignite programmed cell death. Despite biological significance UPR life organism, regulatory transcriptional landscape underpinning stress management largely unmapped, especially crops....
Abstract The societal challenges posed by a growing human population and climate change necessitate technical advances in plant science. Plant research makes vital contributions to society advancing technologies that improve agricultural food production, biological energy capture conversion, health. However, the biology community lacks comprehensive understanding of molecular machinery, including their locations within cells, distributions variations among different cell types, real‐time...