To investigate the mechanism underlying regulatory effect of Met on broiler growth, growth performance, organ development, serum profile, myogenic gene expression, and methylation myostatin exon 1 region in response to dietary status were evaluated. A total 192 one-day-old Arbor Acres chicks housed 3-layer cages a temperature-controlled room with continuous lighting. The temperature was maintained at 32 34°C for first 3 d then reduced by 2 3°C per week final 20°C. Cages randomly allocated...

ABSTRACT Soybean meal (SBM) contains several anti-nutritional factors, which limit the use of soy protein. Exogenous enzyme supplementation and microbial fermentation can reduce factors increase bioavailability nutrients in SBM. In this study, we investigated optimal parameters for solid-state (SSF) SBM by protease combination with probiotics evaluated its effect on growth performance immune response broilers. Protease Bacillus subtilis significantly increased degradation soybean protein...

Immunomodulatory feed additives might offer alternatives to antimicrobial growth promoters in poultry production.This experiment was carried out test the effect of β-glucan supplementation on performance and immune response broilers.Total 160 day-old broilers were randomly assigned 4 treatment groups fed corn-soybean diets containing 0, 0.012, 0.025 or 0.05% supplement a 6 week feeding experiment.Growth performance, antibody titer against New Castle vaccine, lymphocyte blastogensis,...

Viruses belonging to the genus Megalocytivirus in family Iridoviridae are one of major agents causing mass mortalities marine and freshwater fish Asian countries. Outbreaks iridovirus disease have been reported among various species Taiwan. However, genotypes these iridoviruses not yet determined. In this study, seven megalocytivirus isolates from four species: king grouper, Epinephelus lanceolatus (Bloch), barramundi perch, Lates calcarifer silver sea bream, Rhabdosargus sarba (Forsskal),...

White tail disease (WTD) is a serious problem in Macrobrachium rosenbergii hatcheries and nursery ponds Asia. The causative agents have been identified as M. nodavirus (MrNV) its associated extra small virus. This the first report demonstrating MrNV virus displaying WTD signs Taiwan by reverse transcriptase-polymerase chain reaction (RT-PCR). Amplified fragments of 850 425 bp for RNA-1 RNA-2 MrNV, respectively, were obtained RT-PCR. RT-PCR products about 1121 also using different primer...

Marbled eels, Anguilla marmorata (Quoy & Gaimard), cultured in Taiwan exhibited haemorrhage and mortality January 2012. The severely diseased eels bled from the gills showed congestion of central venous sinus gill filaments throughout body similar to viral endothelial cell necrosis eel. In this study, a novel polyomavirus (AmPyV) was isolated using AMPF line established pectoral fin healthy marbled eels. AmPyV found encode long T-antigen orthologous gene. Phylogenetic analysis that closely...

Abstract This study investigates the susceptibilities of SPB cell line to fish viruses including giant seaperch iridovirus ( GSIV ‐ K 1), red sea bream RSIV u), grouper nervous necrosis virus GNNV chum salmon reovirus CSV ) and eel herpesvirus HVA ). 1, u replicated well in cells, with a significant cytopathic effect production. However, cells were refractory. To examine ability stably express foreign protein, expression vectors encoding B 1 2 fused enhanced green fluorescent protein EGFP...

High inclusion of dietary fermented soybean meal (FSBM), as reported in previous studies on poultry production, is less applicable because the increased cost. Therefore, we sought out a new cost-effective application strategy which FSBM was incorporated into broiler diets at low rate (1 to 3%) replace portion other high-protein feedstuff sources, including fish meal, corn gluten and meal. The ADG not adversely affected by partial replacement FSBM. However, birds fed containing 3% had an...

Abstract In Taiwan, a petechial haemorrhage disease associated with mortality has affected marbled eels ( Anguilla marmorata ). The were revealed to be infected adomavirus (MEAdoV, previously recognized as polyoma‐like virus). this study, cell line DMEPF‐5 was established from the pectoral fin of diseased eel. passaged >70 times and thoroughly proliferated in L‐15 medium containing 2%–15% foetal bovine serum at 20–30°C. Transcripts neural adhesion molecule 1 nestin genes, nucleic acids...

A continuous cell line (KF-101) derived from the caudal fin of koi carp Cyprinus carpio was established and characterized. The KF-101 multiplied abundantly in Leibovitz's L-15 medium containing 10% foetal bovine serum at 25° C, subcultured for >90 passages over a period 3 years. Immunocytochemistry revealed that cells contain keratin, junction proteins connexin-43 occludin, ectodermal stem-cell marker Pax-6, but not vimentin. Furthermore, reacted with anti-human DARPP-32 GATA-4 antibodies,...

Abstract A continuous cell line designated BMGB (brown‐marbled grouper brain) was established from the brain tissues of brown‐marbled Epinephelus fuscoguttatus and characterized. cells were identified as astroglial progenitor because they expressed glial fibrillary acidic protein keratin persistently infected by betanodavirus, confirmed through immunocytochemistry, polymerase chain reaction immunoblot analyses. Because few intact virions present in culture fluid, cytopathic effect ( CPE )...

1. Species identification in food products using the bioMerieux FoodExpert-ID® system James Chisholm, Christine M. Conyers, Heather Hird European Food Research and Technology CrossRef

We report here the complete genome sequence of a megalocytivirus strain, GSIV-K1, isolated from farmed giant sea perch (Lates calcarifer) in Kaohsiung, Taiwan. GSIV-K1 causes mortality marine fish, including and groupers. The is nearly identical to orange-spotted grouper iridovirus.

Cell cultures derived from the brain tissues of Aequidens rivulatus (Günther) have been characterized previously. In this study, a continuous cell line ARB8 was further established, and its growth characteristics, transcription susceptibility to fish viruses-including chum salmon reovirus (CSV), marbled eel infectious pancreative necrosis virus (MEIPNV), grouper nervous (GNNV), giant seaperch iridovirus (GSIV), red seabream (RSIV), koi herpesvirus (KHV), anguilla (HVA) polyoma-like...

The aim of this study was to investigate the effect supplementation Cordyceps militaris fermentation products (CMF) on growth performance and immunocompetence piglets. involved three groups animals, which were supplemented with CMF (500, 1000 1500 μg/kg feed), a control group. significantly increased in weaned Bodyweight gain, average daily gain feed intake animals μg CMF/kg higher comparison In addition, only synthesis Th1 cytokines, as indicated by levels IL-2 IFN-γ. piglets fed supplement...