A5087237101- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- SARS-CoV-2 and COVID-19 Research
- Dermatology and Skin Diseases
- Animal Virus Infections Studies
- Diabetes and associated disorders
- Cell Adhesion Molecules Research
- Antifungal resistance and susceptibility
- Extracellular vesicles in disease
- vaccines and immunoinformatics approaches
- Antimicrobial Peptides and Activities
- Immune Response and Inflammation
- Allergic Rhinitis and Sensitization
- Fungal Infections and Studies
- Immunodeficiency and Autoimmune Disorders
- Virology and Viral Diseases
- Escherichia coli research studies
- CAR-T cell therapy research
- Advancements in Transdermal Drug Delivery
- Caveolin-1 and cellular processes
- Ferroptosis and cancer prognosis
- Congenital gastrointestinal and neural anomalies
- Vaccine Coverage and Hesitancy
Thomas Jefferson University
2019-2025
Research Institute of Dallas
2022
Baylor Medical Center at Garland
2019-2022
Baylor University
2018-2021
Baylor Scott & White Health
2018
Translational Genomics Research Institute
2018
University of Minnesota
2007-2016
Biotechnology Institute
2013
University of Minnesota Medical Center
2011-2012
Semmelweis University
2005-2009
Memory CD8 T cells protect against intracellular pathogens by scanning host cell surfaces; thus, infection detection rates depend on memory number and distribution. Population analyses rely isolation from whole organs, interpretation is predicated presumptions of near complete recovery. Paradigmatically, parsed into central, effector, resident subsets, ostensibly defined immunosurveillance patterns but in practice identified phenotypic markers. Because methods ultimately inform models...
Langerhans cells (LCs) are antigen-presenting that reside in the epidermis of skin and traffic to lymph nodes (LNs). The general role these immune responses is not clear because distinct models LC depletion resulted opposite conclusions about their contact hypersensitivity (CHS) responses. While comparing models, we discovered a novel population LCs resides dermis does represent migrating epidermal LCs, as previously thought. Unlike dermal Langerin+ dendritic (DCs) were radiosensitive...
Vaccines based on mRNA-containing lipid nanoparticles (LNPs) are a promising new platform used by two leading vaccines against COVID-19. Clinical trials and ongoing vaccinations present with varying degrees of protection levels side effects. However, the drivers reported effects remain poorly defined. Here we evidence that Acuitas' LNPs in preclinical nucleoside-modified mRNA vaccine studies highly inflammatory mice. Intradermal intramuscular injection these led to rapid robust responses,...
The success of a non-live vaccine requires improved formulation and adjuvant selection to generate robust T cell immunity following immunization. Here, using protein linked TLR7/8 agonist (conjugate vaccine), we investigated the functional properties formulation, cytokines, DC subsets required induce protective multifunctional in vivo. conjugate aggregation elicit potent Th1 CD4+ CD8+ responses. Remarkably, vaccine, through activation TLR7 vivo, led an influx migratory DCs LN increased...
Dendritic cells (DCs) in the intestinal lamina propria (LP) are composed of two CD103(+) subsets that differ CD11b expression. We report here Langerin is expressed by human LP DCs and transgenic langerin drives expression CD103(+)CD11b(+) mice. This subset was ablated huLangerin-DTA mice, resulting reduced Th17 without affecting Th1 or T reg cells. Notably, cognate DC-T cell interactions were not required for development, as this response intact huLangerin-Cre I-Aβ(fl/fl) In contrast,...
Abstract Understanding the function of Langerhans cells (LCs) in vivo has been complicated by conflicting results from LC-deficient mice. Human Langerin-DTA mice constitutively lack LCs and develop exaggerated contact hypersensitivity (CHS) responses. Murine Langerin-diptheria toxin receptor (DTR) allow for inducible elimination Langerin+ dermal dendritic (dDCs) after administration diphtheria toxin, which reduced CHS. When dDCs have partially repopulated skin but are still absent, CHS...
Mice lacking epidermal Langerhans cells (LC) develop exaggerated contact-hypersensitivity (CHS) responses due to the absence of LC during sensitization/initiation. Examination T cell reveals that leads increased numbers hapten-specific CD4 and CD8 but does not alter cytokine expression or development regulatory cells. CHS Ag-specific are in mice which MHC class II is ablated specifically suggesting direct cognate interaction between required for suppression. LC-derived IL-10 also optimal...
The cytokine interleukin-23 (IL-23) is critical for development and maintenance of autoimmune inflammation in nonlymphoid tissues; however, the mechanism through which IL-23 supports tissue-specific immunity remains unclear. In mice, we found that circulating memory T cells were dispensable anamnestic protection from Candida albicans skin infection, tissue-resident (TRM) cell-mediated C. reinfection required IL-23. Administration anti-IL-23 receptor antibody to mice after resolution primary...
The mRNA-LNP vaccine has received much attention during the COVID-19 pandemic since it served as basis of most widely used SARS-CoV-2 vaccines in Western countries. Based on early clinical trial data, these were deemed safe and effective for all demographics. However, latest data raise serious concerns about safety effectiveness vaccines. Here, we review some efficacy identified to date. We also discuss potential mechanism observed adverse events related use whether they can be mitigated by...
Langerhans cells (LCs) are skin-resident dendritic (DC) located in the epidermis that migrate to skin-draining lymph nodes during steady state and response inflammatory stimuli. TGF-β1 is a critical immune regulator highly expressed by LCs. The ability test functional importance of LC-derived complicated requirement for LC development absence LCs mice with an LC-specific ablation or its receptor. To overcome these problems, we have engineered transgenic huLangerin-CreER T2 allow inducible...
After activation, Langerhans cells (LC), a distinct subpopulation of epidermis-resident dendritic cells, migrate from skin to lymph nodes where they regulate the magnitude and quality immune responses initiated by epicutaneously applied antigens. Modulation LC–keratinocyte adhesion is likely be central regulation LC migration. express high levels epithelial cell molecule (EpCAM; CD326), cell-surface protein that characteristic some epithelia many carcinomas has been implicated in...
Vaccines based on mRNA-containing lipid nanoparticles (LNPs) are a promising new platform used by two leading vaccines against coronavirus disease in 2019 (COVID-19). Clinical trials and ongoing vaccinations present with very high protection levels varying degrees of side effects. However, the nature reported effects remains poorly defined. Here we evidence that LNPs many preclinical studies highly inflammatory mice. Intradermal injection these led to rapid robust responses, characterized...
Abstract Langerhans cells (LC) are a subset of skin-resident dendritic (DC) that reside in the epidermis as immature DC, where they acquire Ag. A key step life cycle LC is their activation into mature DC response to various stimuli, including epicutaneous sensitization with hapten and skin infection Candida albicans. Mature migrate skin-draining LN, present Ag CD4 T modulate adaptive immune response. migration thought require direct action IL-1β IL-18 on LC. In addition, TLR ligands C....
To determine the contribution of skin DC subsets in regulation humoral immunity, we used a well-characterized antigen targeting system to limit availability and presentation certain skin-derived subsets. Here show that delivery foreign steady state Langerhans cells (LCs) cDC1s through same receptor (Langerin) led to, respectively, robust versus minimal-to-null immune response. LCs, unlike cDC1s, supported formation germinal center T follicular helper (GC-Tfh) dose-dependently then, likely...