A5028971629- Reproductive tract infections research
- Bacterial Infections and Vaccines
- Aquaculture disease management and microbiota
- Antibiotic Resistance in Bacteria
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Psoriasis: Treatment and Pathogenesis
- Reproductive System and Pregnancy
- Galectins and Cancer Biology
- Dermatology and Skin Diseases
- Autoimmune Bullous Skin Diseases
- Antibiotics Pharmacokinetics and Efficacy
- Fungal Infections and Studies
- Vibrio bacteria research studies
- Urticaria and Related Conditions
- Gut microbiota and health
- Immune Cell Function and Interaction
- Lipid Membrane Structure and Behavior
- Dermatological and Skeletal Disorders
- Cellular Mechanics and Interactions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bacteriophages and microbial interactions
- Syphilis Diagnosis and Treatment
- Nerve injury and regeneration
- NF-κB Signaling Pathways
National Sun Yat-sen University
2019-2025
Sun Yat-sen Memorial Hospital
2016-2024
Sun Yat-sen University
2010-2024
I-Shou University
2024
National Defense Medical Center
2024
Tri-Service General Hospital
2024
Zuoying Armed Forces General Hospital
2024
Min-Hwei College of Health Care Management
2024
Tzu Chi University
2024
Kaohsiung Medical University
2022-2023
Langerhans cells (LCs) are antigen-presenting that reside in the epidermis of skin and traffic to lymph nodes (LNs). The general role these immune responses is not clear because distinct models LC depletion resulted opposite conclusions about their contact hypersensitivity (CHS) responses. While comparing models, we discovered a novel population LCs resides dermis does represent migrating epidermal LCs, as previously thought. Unlike dermal Langerin+ dendritic (DCs) were radiosensitive...
Abstract There are conflicting data in the literature regarding role of epidermal Langerhans cells (LC) promoting skin immune responses. On one hand, LC can be extremely potent APCs vitro, and thought to involved contact hypersensitivity (CHS). other it seems counterintuitive that a cell type continually exposed pathogens at organism’s barrier surfaces should readily trigger T Indeed, depletion model led enhanced hypersensitivity, suggesting they play negative regulatory role. However,...
Psoriasis, an immune-mediated inflammatory skin disorder characterized by a chronically relapsing-remitting course, continues to be primarily managed through topical therapy. While oral administration of tyrosine kinase 2 inhibitors (TYK2i) stands as effective approach for psoriasis treatment, the potential efficacy application TYK2i remains unexplored. Herein, carbomer/alginic acid hydrogel is embedded with borneol (BO) new carrier achieving enhanced transdermal permeation and...
Azole antifungals are the first-line choice for treating candidiasis within a limited antifungal option. However, azole-resistant Candida species have increased rapidly, causing severe clinical threats, especially multidrug-resistant (MDR) isolates. The emergence of auris has also caused global concerns recently. Herein, we evaluated activity taurine-induced silver ions (Tau-Ag), prepared by induction from silver-incorporated mesoporous bioactive glass to address this issue. Our data...
Imipenem-relebactam is a new β-lactam and β-lactamase inhibitor combination to treat carbapenem-resistant gram-negative bacteria infections. However, difference in carbapenem resistant mechanisms existed with geographic variations.To evaluate the susceptibility of imipenem-relebactam 660 carbapenem-nonsusceptible Enterobacteriaceae isolates Taiwan identify vivo efficacy Caenorhabditis elegans model.188 Escherichia coli 472 Klebsiella pneumoniae were collected from national surveillance study...
UV light can be highly beneficial in the treatment of skin disorders such as psoriasis. It is thought to cause immunosuppression by depleting or altering function epidermal Langerhans cells (LC). Our previous studies identified a novel langerin(+) dendritic cell dermis, distinct from LC phenotype, circulation, and function. In this study, we determined role dermal suppression. suppressed CD8 T response both contact hypersensitivity epicutaneous protein immunization, resulted dramatically...
Neisseria gonorrhoeae initiates infection at the apical surface of columnar endocervical epithelial cells in female reproductive tract. These provide a physical barrier against pathogens by forming continuous junctional complexes between neighbouring cells. This study examines interaction gonococci (GC) with polarized We show that viable GC preferentially localize side cell-cell junction endometrial and colonic cells, HEC-1-B T84. In GC-infected are disrupted, junction-associated protein...
The ability to induce humoral and cellular immunity via antigen delivery through the unbroken skin (epicutaneous immunization, EPI) has immediate relevance for vaccine development. However, it is unclear which adjuvants protective memory CD8 T-cell responses by this route, molecular requirements priming intact are not defined. We report that cholera toxin (CT) superior other in its prime T cells control bacterial viral challenges. Epicutaneous immunization with CT does require engagement of...
Microabscess of neutrophils in epidermis is one the histological hallmarks psoriasis. The axis neutrophil-keratinocyte has been thought to play a critical role pathogenesis However, features and mechanism interaction between two cell types remain largely unknown. Herein, we found that blood were increased psoriasis patients, positively correlated with disease severity highly expressed CD66b, but not CD11b CD62L compared healthy controls. Keratinocytes high levels psoriasis-related...
Colonization and disruption of the epithelium is a major infection mechanism mucosal pathogens. The counteracts by exfoliating damaged cells while maintaining barrier function. sexually transmitted bacterium Neisseria gonorrhoeae (GC) infects female reproductive tract primarily from endocervix, causing gonorrhea. However, which GC overcome remains elusive. Using new human tissue model, we demonstrate that can penetrate into endocervix inducing exfoliation columnar epithelial cells. We found...
Psoriasis is a common inflammatory skin disease resulting from an interplay of keratinocytes and immune cells. Previous studies have identified essential role autophagy in the maintenance epidermal homeostasis including proliferation differentiation. However, much less known about autophagy-related proteins cutaneous response. Herein, we showed that ULK1, key autophagic initiator, its phosphorylation at Ser556 were distinctively decreased epidermis lesional psoriasis patients. Topical...
The cervicovaginal microbiota is essential for maintaining the health of female reproductive tract. However, whether status prior to frozen embryo transfer (FET) associates with pregnancy outcomes largely unexplored.Cervical mucus from 29 women who had undergone FET was collected. Microbial composition analyzed using 16 S rRNA gene sequence assess correlation outcomes.CST-categorized Lactobacillus most dominant (41.71%) in pregnant group, while CST-IV-based and BV-related Gardnerella...
Neisseria gonorrhoeae (GC) establishes infection at the mucosal surface of human genital tract, most which is lined with polarized epithelial cells. GC can cause localized as well disseminated infections, leading to various complications. constantly change their structures via phase and antigenic variation, has been implicated a means for establish anatomic locations male female tracks. However, exact contribution each molecule bacterial infectivity remains elusive due variation. Using...
Sexually transmitted infections are a critical public health issue. However, the mechanisms underlying sexually in women and link between infection mechanism wide range of clinical outcomes remain elusive due to lack research models mimicking human vivo. We established cervical tissue explant model mimic local Neisseria gonorrhoeae (GC) infections. found that GC preferentially colonize ectocervix by activating integrin-β1, which inhibits epithelial shedding. selectively penetrate into...
Antibiotic resistance in Neisseria gonorrhoeae (GC) has become an emerging threat worldwide and heightens the need for monitoring treatment failures. N. gonorrhoeae, a gram-negative bacterium responsible gonorrhea, infects humans exclusively can form aggregates during infection. While minimal inhibitory concentration (MIC) tests are often used determining antibiotic development treatment, knowledge of true MIC individual patients how it relates to this laboratory measure is not known. We...
Colistin- and carbapenem-resistant Enterobacteriaceae cases are increasing at alarming rates worldwide. Drug repurposing is receiving greater attention as an alternative approach in light of economic technical barriers antibiotics research. The immunomodulation agent ammonium trichloro(dioxoethylene-O,O’-)tellurate (AS101) was repurposed antimicrobial against colistin- Klebsiella pneumoniae (CRKP). 134 CRKP isolates were collected between 2012 2015 Taiwan. vitro antibacterial activities...
Bacterial pathogens are well equipped to adhere and initiate infection in teleost fish. Fish skin mucus serves as the first barrier against environmental pathogens. The harbors commensal microbes that impact host physiological immunological responses. However, how mucosal microbiota responds presence of remains largely unexplored. Thus, little is known about status prior with noticeable symptoms. In this study, we investigated interactions between fish immune responses Striped catfish...
Neisseria gonorrhoeae (GC) is a obligate human pathogen responsible for gonorrhea, one of the most common sexually transmitted infections. The yearly increased multidrug resistance in GC has led to treatment failure clinically, suggesting an urgent need novel therapy combat this global health issue. AS101 [ammonium trichloro(dioxoethylene-O,O'-)tellurate], tellurium-based compound previously used as immunomodulatory agent, was found have antimicrobial effects against Klebsiella pneumoniae...
The increasing trend of carbapenem-resistant Acinetobacter baumannii (CRAB) worldwide has become a concern, limiting therapeutic alternatives and morbidity mortality rates. immunomodulation agent ammonium trichloro (dioxoethylene-O,O′-) tellurate (AS101) was repurposed as an antimicrobial against CRAB. Between 2016 2018, 27 CRAB clinical isolates were collected in Taiwan. vitro antibacterial activities AS101 evaluated using broth microdilution, time-kill assay, reactive oxygen species (ROS)...