A5045346465- Chemokine receptors and signaling
- Psoriasis: Treatment and Pathogenesis
- Dermatology and Skin Diseases
- Immunotherapy and Immune Responses
- Autoimmune Bullous Skin Diseases
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Pancreatic and Hepatic Oncology Research
- Cutaneous lymphoproliferative disorders research
- Cell Adhesion Molecules Research
- Synthesis and biological activity
- Asthma and respiratory diseases
- Fungal Plant Pathogen Control
- Lymphoma Diagnosis and Treatment
- Cytokine Signaling Pathways and Interactions
- Cancer Genomics and Diagnostics
- Urticaria and Related Conditions
- Cancer Mechanisms and Therapy
- Ion Channels and Receptors
- Cancer Cells and Metastasis
- Immune Cell Function and Interaction
- Radiomics and Machine Learning in Medical Imaging
- T-cell and Retrovirus Studies
- CAR-T cell therapy research
- Caveolin-1 and cellular processes
University of California, Davis
2016-2024
Orlando Regional Medical Center
2024
Medical College of Wisconsin
2010-2023
Seoul National University
2020-2023
National Institutes of Health
2002-2023
Seattle Children's Hospital
2020-2023
Orlando Health
2023
University of California, San Diego
2021
KU Leuven
2021
Sun Yat-sen Memorial Hospital
2021
Dendritic cells (DCs) emigrate to regional lymph nodes (LNs) during immune responses via afferent lymphatic channels. Secondary lymphoid-tissue chemokine (SLC), a CC chemokine, is expressed in secondary lymphoid organs and mediates the chemotaxis of lymphocytes DCs its receptor, receptor 7 (CCR7). By dual-label fluorescence confocal microscopy, we showed MHC class II-positive within SLC-staining channels mouse dermis. SLC was potent vitro chemoattractant for cultured, migratory skin DCs, it...
Immunotherapies targeting aspects of T cell functionality are efficacious in many solid tumors, but pancreatic ductal adenocarcinoma (PDAC) remains refractory to these treatments. Deeper understanding the PDAC immune ecosystem is needed identify additional therapeutic targets and predictive biomarkers for response resistance monitoring. To address needs, we quantitatively evaluated leukocyte contexture 135 human PDACs at single-cell resolution by profiling density spatial distribution...
Background— Little is known about the relationship between intrinsic cardiac nerve activity (ICNA) and spontaneous arrhythmias in ambulatory animals. Methods Results— We implanted radiotransmitters to record extrinsic (ECNA; including stellate ganglion vagal activity) ICNA (including superior left ganglionated plexi ligament of Marshall 6 dogs. Intermittent rapid atrial pacing was performed induce paroxysmal fibrillation or tachycardia. The vast majority (94%) were preceded by coactivated...
Chemokines and chemokine receptors are extensively broadly involved in cancer metastasis. Previously, we demonstrated that epigenetic silencing of the CXCL12 sensitizes breast colon cells to endocrine signaling metastasis distant tissues. Yet, precise mechanism whereby production by tumor regulates dissemination remains unclear. Here, show administration extended survival tumor-bearing mice potently limiting colorectal carcinoma or murine melanoma. Because secreted is a mixture monomeric...
A subset of CC chemokine receptor-6(+) (CCR6(+)), γδ-low (GDL) T cells that express Th17 cytokines in mouse skin participates IL-23-induced psoriasiform dermatitis. We use CCR6-deficient (knockout, KO) and wild-type (WT) mice to analyze trafficking patterns GDL function-blocking mAbs determine the role CCR6 IL-23-mediated Herein, CCL20 was highly upregulated IL-23-injected WT ear as early 24 hours after initial treatment, large numbers CCR6(+) were observed epidermis mice. Anti-CCL20 reduced...
MicroRNA-22 (miR-22) can be induced by beneficial metabolites that have metabolic and immune effects, including retinoic acids, bile vitamin D3, short-chain fatty acids. The tumor suppressor effects of miR-22 been suggested, but whether treats orthotopic hepatocellular carcinoma (HCC) is not established. role in regulating immunity also poorly understood. Our data showed delivered adeno-associated virus serotype 8 effectively treated HCC. Compared with FDA-approved lenvatinib, produced...
The lone CX3C chemokine, fractalkine (FK), is expressed in a membrane-bound form on activated endothelial cells and mediates attachment firm adhesion of T cells, monocytes NK cells. We now show that FK associated with dendritic (DC) epidermis lymphoid organs. In normal human skin, dual-color fluorescence microscopy co-localized expression Langerhans expressing CD1a. tonsil, FK-positive DC CD83, marker for mature DC. Human murine cultured up-regulated mRNA maturation. Furthermore, CD40...
Human melanoma cells frequently express CC chemokine receptor (CCR)10, a whose ligand (CCL27) is constitutively produced by keratinocytes. Compared with B16 murine melanoma, rendered more immunogenic via overexpression of luciferase, that overexpressed both luciferase and CCR10 resisted host immune responses readily formed tumors. In vitro, exposure tumor to CCL27 led rapid activation Akt, resistance cell death induced antigen-specific cytotoxic T cells, phosphatidylinositol-3-kinase...
<h3>Objective</h3> To describe the clinical and histologic manifestations of skin reactions incidentally noted in patients with stage IV melanoma who were treated up to 9 mg/kg a humanized monoclonal antibody reactive against human cytotoxic T-lymphocyte antigen 4 (anti–CTLA-4) as single agent every 3 weeks. <h3>Setting</h3> Single-institution prospective study. <h3>Design</h3> Patients anti–CTLA-4 sole prospectively referred for clinicopathologic characterization occurring during treatment....
V-domain Immunoglobulin Suppressor of T cell Activation (VISTA) is an inhibitory immune-checkpoint molecule that suppresses CD4+ and CD8+ activation when expressed on antigen-presenting cells. Vsir -/- mice developed loss peripheral tolerance multi-organ chronic inflammatory phenotypes. cells were hyper-responsive towards self- foreign antigens. Whether or not VISTA regulates innate immunity unknown. Using a murine model psoriasis induced by TLR7 agonist imiquimod (IMQ), we show deficiency...
Hematogenous dissemination of melanoma is a life-threatening complication this malignant tumor. Here, we identified junctional adhesion molecule-C (JAM-C) as novel player in metastasis to the lung. JAM-C expression was human and murine cell lines, melanoma, well metastatic including lung metastasis. expressed on both B16 cells endothelial promoted transendothelial migration cells. We generated mice with inactivation JAM-C. JAM-C(-/-) endothelial-specific JAM-C-deficient displayed...
Memory T cells (mTC) express multiple chemokine receptors (including CCR4 and CCR6) that may potentially be involved in their arrest on inflamed endothelia. Herein, we specifically addressed whether CCR6 is required for mTC to TNF-alpha-activated human dermal microvascular endothelial (HDMEC) vitro under shear stress conditions. Recombinant liver activation-regulated (LARC)/CCL20 (a ligand) induced firm of cutaneous lymphocyte Ag(+) a flow chamber system using purified substrates....