A5101676494- Peptidase Inhibition and Analysis
- Protein Tyrosine Phosphatases
- Galectins and Cancer Biology
- Parasites and Host Interactions
- Bioactive Compounds and Antitumor Agents
- Cardiac electrophysiology and arrhythmias
- Ginseng Biological Effects and Applications
- Redox biology and oxidative stress
- Zoonotic diseases and public health
- Adenosine and Purinergic Signaling
- Pharmacological Receptor Mechanisms and Effects
- Natural product bioactivities and synthesis
- Histone Deacetylase Inhibitors Research
- HIV/AIDS drug development and treatment
- Protein Degradation and Inhibitors
- ATP Synthase and ATPases Research
- HIV Research and Treatment
- Cardiac Arrhythmias and Treatments
- Spine and Intervertebral Disc Pathology
- Biochemical and Molecular Research
- Amino Acid Enzymes and Metabolism
- Inflammasome and immune disorders
- Chinese history and philosophy
- Ideological and Political Education
- Ubiquitin and proteasome pathways
China Pharmaceutical University
2021-2025
Shandong University
2024-2025
Zhengzhou University
2016-2024
Chengdu Medical College
2024
First Affiliated Hospital of Fujian Medical University
2021-2023
Fujian Medical University
2021-2023
Ministry of Education of the People's Republic of China
2022
Shaanxi University of Chinese Medicine
2020
Henan Provincial Center for Disease Control and Prevention
2019
Nanjing Municipal Center for Disease Control And Prevention
2017
In this study, we used imaging and proteomics to identify the presence of virus-associated cellular proteins that may play a role in respiratory syncytial virus (RSV) maturation. Fluorescence microscopy virus-infected cells revealed virus-induced cytoplasmic inclusion bodies mature particles, latter appearing as filaments. situ electron tomography suggested filaments were complex structures able package multiple copies genome. The particles purified, protein content was analyzed by...
The cullin-RING ubiquitin ligases (CRLs) are responsible for about 20% of cellular protein degradation and regulate diverse processes, the dysfunction CRLs is implicated in human diseases. Targeting has become an emerging strategy treatment Herein, we describe discovery a hit compound from our in-house library further structure-based optimizations, which have enabled identification new triazolo[1,5-a]pyrimidine-based inhibitors targeting DCN1–UBC12 interaction. Compound WS-383 blocks...
FimX is a multidomain signaling protein required for type IV pilus biogenesis and twitching motility in the opportunistic pathogen Pseudomonas aeruginosa. localized to single pole of bacterial cell, unipolar localization crucial correct assembly pili. contains non-catalytic EAL domain that lacks cyclic diguanylate (c-di-GMP) phosphodiesterase activity. It was shown deletion or mutation signature EVL motif affects FimX. However, it not understood how C-terminal could influence considering...
For a significantly long time, the field of health science and public has relied considerably on quantitative experimental evidence to support knowledge. Researchers in social promotion advocate that qualitative research methodologies IPA could be useful tool understand inner world perception for patients with particular illness background. Unlike studies, studies do not seek statistical answers client healing operational activities but how why influences life patients. The purpose study...
Blood-retinal barrier (BRB) breakdown, a pivotal contributor to multiple retinal vascular diseases, manifests as progressive increase in permeability induced by various pathological stimuli. The functional plasticity of endothelial cells can be intricately shaped metabolic alteration. However, little is known about the mechanisms through which disorders trigger dissolution inter-vascular junctions and selective approaches targeting homeostasis. Herein, we identify AMPK-associated fatty acid...
An early repolarization pattern (ERP) has been hypothesized to be arrhythmogenic in experimental studies, but the prognostic significance of ERP general population is controversial. We performed a meta-analysis examine link between and risk sudden cardiac arrest (SCA), death, death from any cause.We literature search using MEDLINE (January 1, 1966 July 31, 2015) EMBASE 1980 with no restrictions. Studies that reported relative (RR) estimates 95% confidence intervals (CIs) for associations...
SHP2 is the first oncogenic tyrosine phosphatase encoded by PTPN11, which plays a significant regulatory role in cancer and inflammation-related diseases. Although allosteric inhibitors have been used phase I/II clinical trials for solid tumors, whether inhibition alleviates psoriasis remains unclear. Here we expressed purified related proteins, established an enzyme activity screening system different conformations of SHP2. We launched iterative medicinal chemistry program identified lead...
Src homology-2-containing protein tyrosine phosphatase 2 (SHP2) is a promising therapeutic target for cancer therapy. In this work, we presented the structure-guided design of 5,6-fused bicyclic allosteric SHP2 inhibitors, leading to identification pyrazolopyrazine-based TK-642 as highly potent, selective, orally bioavailable inhibitor (SHP2
Abstract Cancer cells always have increased ROS levels, thus making them more vulnerable to persistent endogenous oxidative stress. The biochemical difference between cancer and normal could be exploited achieve selective cell killing by exogenous ROS-producing agents. Herein we described a structurally novel steroidal spirooxindole by241 its anticancer efficacy. By241 exhibited potent inhibition against human less toxic cells. concentration-dependently induced apoptosis of MGC-803 EC9706...
We have characterized a linear carbonyl-conjugated polyene generated by the iterative polyketide synthase (CalE8) involved in biosynthesis of 10-membered enediyne core calicheamicin. The results provide insight into mysterious biosynthetic mechanism unique enediyne. differs from precursor for 9-membered enediyne, suggesting that divergence starts at PKS stage.
To determine the efficacy and underlying mechanism of Astragalus polysaccharides (APS) on plasma lipids in hypercholesterolemia hamsters. The effect APS (0.25g/kg/d) liver lipids, fecal bile acids neutral sterol, cholesterol absorption synthesis, HMG-CoA reductase activity, gene protein expressions small intestine was investigated twenty-four Treatment periods lasted for three months. significantly lowered total by 45.8%, triglycerides 30%, low-density lipoprotein-cholesterol 47.4%,...
Lysine-specific demethylase 5B (KDM5B) has been recognized as a potential drug target for cardiovascular diseases. In this work, we first found that the KDM5B level was increased in mouse hearts after transverse aortic constriction (TAC) and Ang II-induced activated cardiac fibroblasts. Structure-based design further optimizations led to discovery of highly potent pyrazole-based inhibitor TK-129 (IC50 = 0.044 μM). reduced activation fibroblasts vitro, exhibited good PK profile (F 42.37%),...
Type IV pili (T4P) are polar surface structures that play important roles in bacterial motility, biofilm formation, and pathogenicity. The protein FimX its orthologs known to mediate T4P formation the human pathogen Pseudomonas aeruginosa some other species. It was reported recently FimX(XAC2398) from Xanthomonas axonopodis pv. citri interacts with PilZ(XAC1133) directly through nonenzymatic EAL domain of FimX(XAC2398). Here we present experimental data reveal strong interaction between is...
Esophageal cancer (EC) is an aggressive malignancy and the most common solid tumor of gastrointestinal tract all over world, with high incidence in Asia. The current study was designed to investigate anticancer efficacy mechanism that involved action a natural ent-kaurene diterpenoid, JDA-202, targeting EC.We found antioxidant protein peroxiredoxin I (Prx I) upregulated human EC tissues as well cell lines. novel compound isolated from Isodon rubescens (Labiatae), proved possess strong...