A5013013739- CRISPR and Genetic Engineering
- CAR-T cell therapy research
- Neurogenetic and Muscular Disorders Research
- Prenatal Screening and Diagnostics
- Virus-based gene therapy research
- Pluripotent Stem Cells Research
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- 3D Printing in Biomedical Research
- Optical Network Technologies
- Reproductive Biology and Fertility
- Advanced Fiber Optic Sensors
- Biochemical and Molecular Research
- Stock Market Forecasting Methods
- Market Dynamics and Volatility
- Genetic Neurodegenerative Diseases
- Immunodeficiency and Autoimmune Disorders
- Congenital Anomalies and Fetal Surgery
- Ferroptosis and cancer prognosis
- Hemophilia Treatment and Research
- Renal and related cancers
- Photonic Crystal and Fiber Optics
- Cancer, Lipids, and Metabolism
- RNA Interference and Gene Delivery
- Forecasting Techniques and Applications
First Affiliated Hospital of Zhengzhou University
2022-2025
Affiliated Hospital of Nantong University
2023
Nantong University
2023
Yanshan University
2023
Central South University
2016-2018
University of Virginia
2015
Severe combined immunodeficiency (SCID) is a set of rare monogenic inherited diseases that together represent the most severe form primary disease phenotype. Preimplantation genetic testing for defects (PGT-M) an effective reproductive technology strategy to prevent disease-causing gene mutations from being transmitted offspring. The aim this study was report use PGT-M based on karyomapping in four families avoid birth SCID children. Four couples underwent due SCID. started with biopsy...
Induced pluripotent stem cells (iPSCs) are a promising source of mesenchymal (MSCs) for clinical applications. In this study, we transformed human iPSCs using non-viral vector carrying the IL24 transgene pHrn-IL24. PCR and southern blotting confirmed integration into rDNA loci in four 68 iPSC clones. We then differentiated high expressing IL24-iPSC clone MSCs (IL24-iMSCs) that showed higher expression culture supernatants cell lysates than control iMSCs. IL24-iMSCs efficiently osteoblasts,...
Hemophilia B (HB) is an X-linked recessive bleeding disorder, caused by F9 gene deficiency. Gene therapy combined with the CRISPR/Cas9 technology offers a potential cure for hemophilia B. Now Cas9 nickase (Cas9n) shows great advantage in reducing off-target effect compared wild-type Cas9. In this study, we found that multicopy ribosomal DNA (rDNA) locus, homology directed recombination (HDR) efficiency induced sgRNA-Cas9n was much higher than sgRNA-Cas9, meanwhile without six predicted...
Background: Oocyte maturation arrest is a disease that produces immature oocytes and cannot be mature after culturing in vitro, which leads to female primary infertility. We aimed summarize nine representative patients our center retrospectively analyze the genetic variants clinical characteristics of oocyte arrest. Methods: This study examined analyzed families with Whole-exome sequencing (WES) probands was performed detect pathogenic variants. Sanger verified WES findings available...
Disheveled, EGL-10, and pleckstrin (DEP) domain-containing protein 5 (DEPDC5) is a component of GTPase-activating (GAP) activity toward the RAG complex 1 (GATOR1) protein, which an inhibitor amino acid-sensing branch mammalian target rapamycin (mTORC1) pathway. GATOR1 variations were reported to correlate with familial focal epilepsy variable foci (FFEVF). With wide application whole exome sequencing (WES), more in
Reporter embryonic stem cell (ESC) lines with tissue-specific reporter genes may contribute to optimizing the differentiation conditions in vitro as well trafficking transplanted cells vivo. To optimize and monitor endothelial (EC) specifically, here we targeted enhanced green fluorescent protein (EGFP) gene at junction of 5'UTR exon2 specific marker CD144 using TALENs human ESCs (H9) generate a EGFP-CD144-reporter ESC line. The expressed EGFP increasingly specifically without unexpected...
Long-term investors are interested in identifying the characteristics of companies that likely to triple value over next five years, which equates return approximately 25 percent per year period. Such known as compounders due their high compounded rate return. This paper reports on an analysis corporate and market data undertaken with goal specific tend separate from other equities. We consider a wide ranging set designed bear financial performance. Characteristics constructed using...
Withdrawal Statement The authors have withdrawn their manuscript owing to adjustments been made the content. Therefore, do not wish this work be cited as reference for project. If you any questions, please contact corresponding author.